"Is COVID-19 a biological weapon?" We publish the next part of the book by the father of modern nuclear cardiology and nuclear medicine in the United States, Dr. Richard M. Fleming.
"In May 2007, a patent was issued for the isolation of the SARS-CoV-1 virus infecting humans. The patent owner— the party that would make financial gains from the patent — was the U.S. Department of Health and Human Services.
This isolation not only genetically identified the virus, but also adapted a polymerase chain reaction (PCR) test to detect SARSCoV-1. In April 2020, the FDA issued a comprehensive emergency approval for PCR testing to detect SARS-CoV-2.
2007: Studies show that inserts placed in spike proteins allow SARS-CoV to infect human cells
The study, published by Dr Zhengley and researchers from the Australian Animal Health Laboratory, examined SARS-like coronaviruses (SL-CoV) found in horseshoe bats and SARS-related coronavirus (SARS-CoV).
Although they found significant amounts of similar genetic material, they also found that the spike protein SL-CoV cannot bind to human ACE2 receptors. Zhengleee and other scientists have discovered that the spike protein in SL-CoV cannot use ACE2 receptors to infect human cells.
However, when they inserted amino acids not found in natural viruses into the N-terminal domain through manipulation of enhanced functionality (chimeric), they found that they could produce viruses capable of infecting human cells.
Is COVID-19 a biological weapon? Chapter 2, Part 2
2001: Barik and his colleagues apply for a patent for gene manipulation
By May 2001, Barik and Yount had applied for a patent allowing them to control and profit from genetic manipulation of plants, animals, bacteria and viruses, including coronaviruses. The patent was granted on July 15, 2003, and this patent included research conducted with the financial support of U.S. taxpayers [17].
- 2003: The Creation of the Coronavirus
In 2003, Barik and others published a study [18] funded by NIH grants AI23946, GM63228 and AI26603, showing that they can "save" SARSCoV Urbani viruses using reverse genetics [19]. By taking the cDNA segments and shutting them down, they were able to clone the SARS viruses entirely. It was then shown that these clones are capable of infecting VeroE6 cells [20].
When N transcripts (nucleocapsid) were turned on, the infection was more severe. When the cells were not infected with this coronavirus (control cells), staining with antibodies to the virus was not observed.
The same study showed that cysteine proteinase inhibitors can prevent cell infection. It also pointed out that researchers can manipulate the genes of the virus, according to the researchers:
Current data show that the cysteine proteinase inhibitor E64-d can suppress SARS virus replication at any time during infection. ... The availability of a full-size cDNA of the SARS genome should allow genetic manipulation of the replicase genome, providing a new understanding of the role of specific proteolytic cleavages and replicase proteins during viral replication [21].
The first part of this finding in their study clearly demonstrated interest in further genetic manipulation of the virus. The last part is crucial to understanding the treatment options for SARS-CoV-2. Exposure to clindamycin on this transmembrane protease serine 2 is one of the reasons I decided to include it in the treatment of patients infected with SARSCoV-2 and those experiencing an inflammatory thrombosis response (ITR) known as COVID-19 [22].
The structural nucleocapside (N) protein of SARS viruses appears to not only play a significant role in increasing infectivity, but it has also been shown to insert (reverse transcribe) its genetic sequence into human DNA for SARSCoV-2 – this has also been funded by the NIH, grants 1U19AI131135-01, 5R01MH104610-21. Since then, this reverse transcription (OT) has been shown to occur in all but three of the twenty-three pairs of human chromosomes [23].
2006: Chimeric cDNA (enhanced functionality) derived from hepatitis C, HIV-1, SARS-CoV-1 and SARS-CoV-2 viruses
In 2006, Chinese researchers joined four target segments of cDNA together to form a single RNA sequence 1200 nucleotides long [24].
This chimeric (enhanced functionality) sequence included a combination of hepatitis C virus (HCV), human immunodeficiency virus 1 (HIV-1), SARS-CoV-1, and SARSCoV-2. This genetic sequence is shown in the appendix. This study was funded by the Fujian government [25] (grant number 2003Y004).
2007: Patent for the SARS-CoV (SARS-CoV-1) genome [26] granted to the U.S. Department of Health and Human Services
In May 2007, a patent was issued for the isolation of the SARS-CoV-1 virus infecting humans [27]. The patent owner— the party that would make financial gains from the patent — was the U.S. Department of Health and Human Services (HHS). This isolation not only genetically identified the virus, but also adapted a polymerase chain reaction (PCR) test to detect SARSCoV-1. In April 2020, the FDA issued a comprehensive emergency use authorization (EUA) for PCR testing to detect SARS-CoV-2 [28].
2007: Studies show that inserts placed in spike proteins allow SARS-CoV to infect human cells
The study, published by Dr. Zhengley and researchers at the Australian Animal Health Laboratory, examined SARS-like coronaviruses (SL-CoV) found in horseshoe bats and SARS-related coronavirus (SARS-CoV) [29]. Although they found significant amounts of similar genetic material, they also found that the spike protein SL-CoV cannot bind to human ACE2 receptors. Zhengleee and other scientists have discovered that the spike protein in SL-CoV cannot use ACE2 receptors to infect human cells. However, when they inserted amino acids not found in natural viruses into the N-terminal domain through manipulation of enhanced functionality (chimeric), they found that they could produce viruses capable of infecting human cells.
2010: Shi Zhengli-Li conducts chimeric experiments showing that the SARS-CoV spike protein cannot bind to human cell receptors ACE2
In 2010, Shi Zhengleee conducted a chimeric study on SARS-CoV-1 (then called SARS-CoV), including combining it with the HIV pseudovirus to study the virus's binding ability to human ACE2 receptors [30]. Their work, in particular, involved modifying (through mutagenesis) spike proteins to determine how to increase the binding of spike proteins to the ACE2 receptor.
This study showed that insertion of proline-arginine-arginine-alanine (PRRA) [31] is not critical for binding SARS-CoV-2 to ACE2 receptors in human cells. As noted in this published study (co-authored by Dr. Zhengley of the Wuhan Institute of Virology, researchers from Australia, and the University of Minnesota School of Medicine), not only can the spike protein [found in coronaviruses in] horseshoe bats not bind to ACE2 receptors, but also these differences, along with differences in civets [32], highlight a critical missing link in the zoonotic theory of the origin of SARS-CoV-2:
However, although the genetically related SARS-like coronavirus (SL-CoV) has been identified in horseshoe bats of the genus Rhinolophus [5, 8, 12, 18], its spike protein could not use the human protein ACE2 (hACE2) as a receptor. A careful study of the crystal structure of human RBD SARS-CoV in combination with hACE2 suggests that shortening the receptor-binding motif (RBM) region of the SL-CoV spike protein cancels its hACE2-binding ability [7, 10], and therefore SL-CoV, recently discovered in horseshoe bats, is unlikely to be a direct ancestor of SARS-CoV in humans.
In addition, it has been shown that the human SARS-CoV spike protein and the SARS-CoV spike protein closest to it in civets could not use the ACE2 horseshoe bat (R. pearsoni) as a receptor [13], highlighting a critical missing link in the SARSCoV transmission chain from bat to civet/human. [Emphasis added.]
2013: SARS-CoV was linked to fatal blood clotting in the lungs
Barik and his collaborators discovered in 2013 that SARSCoV — in a study funded by NIAID, NIH, the National Center for the Advancement of Interdisciplinary Research (NIH/NCATS) and HHS (grants HHSN272200800060C; 5UL1RR024140) — had four critical genes that were expressed after SARS-CoV infection:
The results of these studies demonstrate that there is a delicate balance between host coagulation and fibrinolysin pathways regulating pathological disease outcomes, including diffuse alveolar damage and acute lung damage, after infection with highly pathogenic respiratory viruses such as SARS-CoV [33].
They went on to show that it is crucial for infection and lung damage:
The cascade of urogonase reactions had a significant impact on both lung pathology and the overall pathogenesis of SARS-CoV.
As shown by studies of lung tissue, the greater the viral load (more pronounced infection), the greater the damage to the lungs by fibrin (blood clotting) [34].
2014: Barik eschews for international patent to change coronavirus spike protein
In March 2014, Professor Barik applied for an international patent for methods for producing and composition of chimeric (enhancing functionality) proteins-spikes of the coronavirus. As shown in the following figure, this invention (patent) was created with the support of the NIH grant U54AI057157, which further demonstrates that the US federal government is funding research to enhance the functionality of the coronavirus spike protein.
References to Chapter 2
1. R. A. Flavell et al., “Site-Directed Mutagenesis: Generation of an Extracistronic Mutation in Bacteriophage QßRNA,” Journal of Molecular Biology 89, no. 2 (October 25, 1974): 255–72.
2. Charles Weissmann, “The End of the Road,” Landes Bioscience 6, no. 2 (2012): 970194, y.
3. M. M. Lai et al., “Recombination between Nonsegmented RNA Genomes of Murine Coronaviruses,” Journal of Virology 56 (1985): 449–56.
4. Kary B. Mullis et al., Process for Amplifying, Detecting, and/or Cloning Nucleic Acid Sequences, US Patent 4,683,195, 21; Tyler Durden, “Caught Red-Handed: CDC Changes Test Thresholds to Virtually Eliminate New COVID Cases among Vaxx’d,” ZeroHedge.com, May 23, 2021, https://www.zerohedge.com/covid-19/caught-red-handed-cdc-changes-test-th....
5. R. M. Fleming, “The Pathogenesis of Vascular Disease,” in Textbook of Angiology, ed. John C. Chang (New York: Springer Verlag, 1999), 787–98. doi:10.1007/978-1-4612-1190-7_64.
6. Chronic inflammatory diseases include coronary artery disease (heart disease), vascular disease of the brain (e.g., stroke), hypertension (high blood pressure), diabetes, obesity, and cancer.
7. 20/20, April 16, 2004, “Hidden Heart Disease: Could a Simple, Inexpensive Test Save Your Life?,” aired April 16, 2004, on ABC; https://rumble.com/vkb7n1-inflammation-and-heart-disease-2020.html.
8. Richard M. Fleming and Matthew R. Fleming, “FMTVDM Quantitative Nuclear Imaging Finds Three Treatments for SARSCoV-2,” Biomedical Journal of Scientific and Medical Research 33, no. 4 (February 8, 2021): 26041– 83, doi:10.26717/BJSTR.2021.33.005443, https://biomedres.us/fulltexts/BJSTR.MS.ID.005443.php.
9. F. Almazán, “Engineering the Largest RNA Virus Genome as an Infectious Bacterial Artificial Chromosome,” Proceedings of the National Academy of Sciences 97, no. 10 (2000): 5516–21.
10. Ibid.
11. Boyd Yount, Kristopher M. Curtis, and Ralph S. Baric, “Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model,” Journal of Virology 74, no. 22 (December 22, 2000): 10600–11.
12. Ibid.
13. Volker Thiel et al., “Infectious RNA Transcribed In Vitro from a cDNA Copy of the Human Coronavirus Genome Cloned in Vaccinia Virus,” Journal of General Virology 82 (June 2001): 1273–81.
14. MRC-5 cells (also known as the No. 5 Cell Line of the Medical Research Council) were grown from the lung tissue of a 14-week-old abotened Male Caucasoid embryo.
15. One way to do this is through clusters of short palindromic repeats at regular intervals. One method for doing this is called Clusters of Regularly Interspaced Short Palindromic Repeats (CRISPR).
16. Bredenbeek and Rice, 1992; Mandl et al., 1998.
17. Boyd Yount, Kristopher M. Curtis, and Ralph S. Baric, “Strategy for Systematic Assembly of Large RNA and DNA Genomes: Transmissible Gastroenteritis Virus Model,” Journal of Virology 74, no. 22 (December 22, 2000): 10600–11.
18. Boyd Yount et al., “Reverse Genetics with a Full-Length Infectious cDNA of Severe Acute Respiratory Syndrome Coronavirus,” Proceedings of the National Academy of Sciences 100, no. 22 (October 28, 2003): 12995–13000.
19. Ability to use reverse transcription to obtain cDNA from mRNA. The ability to use reverse transcription to make cDNA from mRNA.
20. Vero E6 cells are a line of kidney epithelial cells taken from the African green monkey on March 27, 1962.
21. Boyd Yount et al., “Reverse Genetics with a Full-Length Infectious cDNA of Severe Acute Respiratory Syndrome Coronavirus,” Proceedings of the National Academy of Sciences 100, no. 22 (October 28, 2003): 12995–13000.
22. NCT04349410. Richard M. Fleming and Matthew R. Fleming, “FMTVDM Quantitative Nuclear Imaging Finds Three Treatments for SARS-Cov-2,” Biomedical Journal of Scientific and Medical Research 33, no. 4 (February 8, 2021): 26041–83, doi: 10.26717/BJSTR.2021.33.005443, https://biomedres.us/fulltexts/BJSTR.MS
.ID.005443.php;Canrong Wu et al., “Analysis of Therapeutic Targets for SARS-CoV-2 and Discovery of Potential Drugs by Computational Methods,” Acta Pharmaceutica Sinica B 10, no. 5 (May 2020): 766–88; Satish Sagar et al., “Bromelain Inhibits SARS-CoV-2 Infection via Targeting ACE-2, TMPRSS2, and Spike Protein,” Clinical and Translational Medicine 11, no. 2 (February 2021): e281.
23. Liguo Zhang et al., “Reverse Transcribed SARS-CoV-2 RNA Can Integrate into the Genome of Cultured Human Cells and Can Be Expressed in Patient-Derived Tissues,” Proceedings of the National Academy of Sciences 118, no. 21 (May 25, 2021): e2105968118.
24. Qiuying Huang et al., “Preparation of Chimeric Armored RNA as a Versatile Calibrator for Multiple Virus Assays,” Clinical Chemistry 52, no. 7 (July 1, 2006): 1446–48 and Supplement A.
25. Commonly known as the People’s Revolutionary Government of the Republic of China. Since 2018, it has been superseded and duties transferred to the Kinmen-Matsu Joint Services, the National Development Council, and other ministries for the Executive Yuan (executive branch) of Taiwan.
26. Genome is the entire genetic code of an organism—for example, the SARSCoV-1 virus.
27. US Department of Health and Human Services, Coronavirus Isolated from Humans, Patent 7,220,852 B1, issued May 22, 2007.
28. US Food and Drug Administration, “In Vitro Diagnostics EUAs — Molecular Diagnostic Tests for SARS-CoV-2,” last updated July 19, 2021, https://www.fda.gov/medical-devices/coronavirus-disease-2019-covid-19-em....
29. Wuze Ren et al., “Difference in Receptor Usage between Severe Acute Respiratory Syndrome (SARS) Coronavirus and SARS Like Coronavirus of Bat Origin,” Journal of Virology 82, no. 4 (February 2008): 1899–1907.
30. Yuxuan Hou et al., “Angiotensin-Converting Enzyme 2 (ACE2) Proteins of Different Bat Species Confer Variable Susceptibility to SARS-CoV Entry,” Archives of Virology 155, no. 10 (October 2010): 1563–69, doi: 10.1007/ s00705-010-0729-6.
31. PRRA ― буквенное обозначение четырех аминокислот, в частности пролина, аргинина, аргинина и аланина.
32. Asian-African animal species frequently used by those like Daszak who attempt to explain SARS-CoV-2 as a naturally occurring (zoonotic) virus.
33. Lisa E. Gralinski et al., “Mechanisms of Severe Acute Respiratory Syndrome Coronavirus-Induced Acute Lung Injury,” mBio 4, no. 4 (August 6, 2013): e00271-13, doi:10.1128/mBio.00271-13.
34. Ibid.
35. TMPRSS2 is transmembrane protease serine 2. This enzyme, which breaks apart proteins, is found in humans. The gene for TMPRSS2 is found on human chromosome 21. See https://doi.org/10.1006/geno.1997.4845.
36. Xue Han et al., “Structure of the S1 Subunit C-Terminal Domain from Bat-Derived Coronavirus HKU5 Spike Protein,” Virology 507 (July 2017): 101–9.
37. Y. Yang et al., “Receptor Usage and Cell Entry of Bat Coronavirus HKU4 Provide Insight into Bat-to-Human Transmission of MERS Coronavirus,” Proceedings of the National Academy of Sciences 111, no. 34 (August 26, 2014): 12516–21.
38. R. M. Fleming, “The Pathogenesis of Vascular Disease,” in Textbook of Angiology, ed. John C. Chang (New York: Springer Verlag, 1999), 787–98. https://doi.org/10.1007/978-1-4612-1190-7_64.
39. William T. Gibson et al., “ACE 2 Coding Variants: A Potential XLinked Risk Factor for COVID-19 Disease,” BioRXiv (April 14, 2020), https://doi.org/10.1101/2020.04.05.026633.
40. Wanbo Tai et al., “Characterization of the Receptor-Binding Domain (RBD) of 2019 Novel Coronavirus: Implications for Development of RBD Protein as a Viral Attachment Inhibitor and Vaccine,” Cellular & Molecular Immunology 17, no. 6 (June 2020): 613–20.
41. SARS-CoV, now referred to as SARS-CoV-1, was first reported in China in February of 2003. See https://www.cdc.gov/sars/index.html.
42. “Lethal Deception,” Rumble.com, April 22, 2021. Video, 1:31:19, https://rumble.com/vfy3xf-lethal-deception.html.
43. Boyd Yount et al., “Reverse Genetics with a Full-Length Infectious cDNA of Severe Acute Respiratory Syndrome Coronavirus,” Proceedings of the National Academy of Sciences 100, no. 22 (October 28, 2003): 12995–13000; M. M. Becker, “Synthetic Recombinant Bat SARS-Like Coronavirus Is Infectious in Cultured Cells and in Mice,” Proceedings of the National Academy of Sciences 105, no. 50 (December 2008): 19944–49.
44. V. D. Menachery et al., “A SARS-Like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence,” Nature Medicine 21, no. 12 (2015): 1508–13.
45. “New SARS-Like Virus Can Jump Directly from Bats to Humans, No Treatment Available,” Gillings School of Global Public Health, November 9, 2015, https://sph.unc.edu/sph-news/new-sars-like-virus-can-jump-directly-from-....
46. That’s something Dr. Fauci now denies
despite the publication acknowledging NIAID funding.
47. V. D. Menachery et al., “Corrigendum: A SARS-Like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence,” Nature Medicine 22, no. 4 (2016): 446.
48. Gordon Duff, “Documentary Proof: University of North Carolina Generated COVID-19,” State of the Nation (website), April 28, 2020, https://stateofthenation.co/?p=12668.
49. V. D. Menachery et al., “A SARS-Like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence,” Nature Medicine 21, no. 12 (2015): 1508–13, doi:10.1038/nm.3985.
50. Dr. Carlo Urbani was an Italian microbiologist and physician who first identified Severe Acute Respiratory (SARS) viruses and warned the WHO.
51. A = adenine, C = cytosine, G = guanine, T = thymine, and U (not shown here) = uracil.
52. Richard M. Fleming, Unmasking COVID — Part 1 (self-pub., 2020), Kindle, https://www.amazon.com/Unmasking-CoViD-Dr-Richard-Fleming-ebook/dp/B08N5....
53. V. D. Menachery et al., “A SARS-Like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence,” Nature Medicine 21, no. 12 (2015): 1508–13.
54. V. D. Menachery et al., “Corrigendum: A SARS-Like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence,” Nature Medicine 22, no. 4 (2016): 446.
55. Bill Gates said it would be a “‘tragedy’ to pass up a controversial, revolutionary gene-editing technology.” Article by Kevin Loria April 12, 2018 Insider.
56. Janet Levy, “Mengele’s Unethical Research Was Part of Existing Beliefs, Practice,” Jerusalem Post, August 6, 2020, https://www.jpost.com/israel-news/culture/mengeles-unethical-research-wa....
57. Y. Yang et al., “Two Mutations Were Critical for Bat-to-Human Transmission of Middle East Respiratory Syndrome Coronavirus,” Journal of Virology 89, no. 17 (2015): 9119–23.
58. Ibid.
59. C. Calisher et al,, “Statement in Support of the Scientists, Public Health Professionals, and Medical Professionals of China Combating Covid-19,” Lancet 395 (2020): e42-43.
60. Zoonotic viruses are viruses spread from animals to people. See https://www.cdc.gov/onehealth/basics/zoonotic-diseases.html.
61. Andrew Kerr, “US Researcher with Chinese Ties Admits He Convinced WHO Team That Missing Wuhan Lab Data Was Irrelevant,” Daily Caller, March 10, 2021, https://dailycaller.com/2021/03/10/who-investigators-wuhan-lab-database-....
62. Sam Husseini, “EcoHealth Alliance Has Hidden Almost $40 Million in Pentagon Funding and Militarized Pandemic Science,” Independent Science News (website), December 16, 2020, https://www.independentsciencenews.org/news/peter-daszaks-ecohealth-alli....
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John F. Kennedy Last Breakfast
1963.