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Wastewater-based epidemiology WBE has been widely used as a complementary method for estimating consumption of illicit drugs in the population. Temporal drug consumption estimates derived from WBE can provide important information for law enforcement and public health authorities in understanding changes in supply and demand of illicit drugs, but currently lacking in China. In this study, influent wastewater samples from a municipal sewage treatment plant in Guangzhou, China were collected for 8 weeks to investigate the temporal change in consumption of six illicit drugs in the catchment. The results indicated that methamphetamine and ketamine were the dominant illicit drugs in Guangzhou with the per capita use of No distinct weekly patterns were observed for illicit drug consumption in Guangzhou, indicating that drug users are likely to be regular ones. The considerable decrease in drug consumption observed in October compared with January and May was possibly the consequence of law enforcement action. Keywords: Intervention; Ketamine; Methamphetamine; Population normalized consumption; Temporal variation; Wastewater-based epidemiology. Abstract Wastewater-based epidemiology WBE has been widely used as a complementary method for estimating consumption of illicit drugs in the population.

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