User`s Manual PDF e3434203
RapidSeqSmallRNASamplePrepKit1011V2UsersManualandInstructionsProductRapidSeqSmallRNASamplePrepKitCatalogNumberKS071012KS071012IKSO71012IIKSO71012IIIandKS071012IVIntroductionSmallRNAincludesmicroRNAmiRNAncRNAsiRNAsnoRNApiRNArasiRNAandmanymoreItisalargefamilyofregulatorymoleculesinorganismsandplaysanimportantroleindevelopmentanddiseaseNextGenerationSequencingNGSisapowerfultooltoidentifyandquantitativelyanalyzetheentirepopulationofsmallRNAsmiRNAsareendogenousregulatorsofgeneexpressionthatareencodedinthegenomesofanimalsplantsandvirusesMaturemiRNAsare1824ntsinglestrandedmoleculesthatbecomeincorporatedintotheRNAinducedsilencingcomplexRISCRISCmediatesdownregulationofgeneexpressionthroughtranslationalinhibitiontranscriptcleavageorbothThismanualaimstoprepareNGSlibrariesforsubsequentclustergenerationusingpurifiedsmallRNAortotalRNAwhichcontainssmallRNAfragmentsasinputTheprotocolincludesstepsforadaptersligationreversetranscriptionPCRamplificationandsizeselectionbygelpurificationtogeneratealibraryproductcompatiblewithilluminaNGSplatformFigure1ThemethodinthiskitligatesadaptersdirectionallytomiRNAsbasedontheirnaturestructurewitha5phosphateanda3hydroxylgroupFigure1WorkflowChartofSmallRNANGSLibraryConstructionPurifiedSmallRNAorTotalRNAContainsSmallRNAFragments3AdapterLigationVAARNAA5AdapteEiigationMAAARAL8979FirstStrandSynthesisMananpooocscocacacacaogoReverseTranscriptionAmplificationwithIndexPCROGelPurificationBioChainalsoprovidesothertoolsandservicestoresearchersinterestedinstudyingsmallRNAPleasecontactBioChainTechnicalSupportforfurtherdetailsFeatureseSimpleworkflowmostcomponentsaresuppliedasreadytousesupermixtureswhichreducessetuptimeandliquidhandlingstepsTable1eGreatperformancecomparableyieldwithbenchmarksfreshmademixtureseWidedynamicrangetotalRNAinputcouldbedownto100ngBioChainInstituteIncWebsitewwwbiochaincomt8887622568f5107835386emailinfobiochaincomRapidSeqSmallRNASamplePrepKitTable1SavingsinmanualtimeandeffortwithBioChainmethodTotalprocesstime1hr15minProtocolHandsontime3AdapterLigation5AdapterLigationFirstStrandSynthesisCSizeselectionPurificationTotalworkflowtimeApplicationseSmallRNAdetectionandquantificationeSmallRNAdiscoveryeMIRNAexpressionprofilingeMiRNArelatedfunctionalassessmentandvalidationDescription1011V2ComponentsinthiskitarepreparedwithpurechemicalstoconstructNGSlibrariescompatiblewith
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