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As common psychotropic drugs, ketamine KET and amphetamine AMP are often consumed by drug users at the same time, which seriously threatens people's health. The antibodies were attached on Au Ag core—shell nanoparticles embedded with different Raman reporters as the detection substrates. The labelled antigens KET—BSA and AMP—BSA were linked to carboxyl magnetic beads, and by adopting the principle of competitive immunoassay, the quantitative detections of ketamine and amphetamine were realized at the same time by detecting the Raman signals at different characteristic peaks on the magnetic beads. This method had the advantages of simple, rapid operation, and high sensitivity, and can realise double indicator simultaneous detection, which provided a more favorable scientific basis for preventing or reducing drug abuse, and identifying and monitoring drug users. At present, the commonly used detection methods of ketamine and amphetamine mainly include high-performance liquid chromatography, gas chromatography, mass spectrometry, 4—7 near-infrared spectroscopy, 8 electrochemical method, 9—11 fluorescence analysis method, 12,13 the colorimetric method 14 and chemiluminescence method. In fact, most drug users are not limited to taking only one drug, and they are frequently taking two or more drugs at the same time to get more mental stimulation. The reported methods listed above can only perform quantitative detection of one drug in one injection, and it is difficult to achieve simultaneous detection of two or more drugs. Therefore, it is one of the current research hotspots to establish a method of high sensitivity, simple and fast operation, which is capable of quantitatively detecting multiple drugs at one time. Raman spectroscopy is a kind of fingerprint spectrum which can characterize the vibration of molecular structure. The huge Raman scattering effect on rough surfaces of precious metal can enhance Raman signals by several orders of magnitude. It provides the possibility to achieve the trace detection of substance molecules. In fact, the detection performance of SERS mainly depends on the enhancement ability of the substrate. Because gold and silver nanostructures can exist very stably in the air and have a surface plasmon resonance effect superior to other precious metals, they are widely used as SERS substrates for clinical test and analysis of psychotropic drugs. In this study, a highly sensitive simultaneous detection of double indicators including ketamine and amphetamine was achieved by SERS competitive immunoassay. The higher the concentration of the sample, the less the labelled antigen was captured by the antibody. The magnetic beads are gathered under the action of an external magnetic field, by detecting the Raman signals at the characteristic peaks of 4MBA and XP on the magnetic beads, the rapid and highly sensitive simultaneous detection of ketamine and amphetamine can be achieved Fig. After 20 times of repeated determination of the blank sample, the average M and the standard deviation SD were obtained. The results show that when the sample concentration was 0. The limit of detection in this study was far lower than the cut-off value, which proved that this study was competitive. By comparing the detection performances of KET and AMP in this study with other reported methods Table 4 , it is proved that this study has a good or at least comparable limit of detection and that the detection sensitivity of this study is significantly higher than other SERS methods. It is worth noting that the final goal of this study was the simultaneous detection of KET and AMP, while the reported methods were single-index detection. DOI: Received 8th August , Accepted 19th September Abstract As common psychotropic drugs, ketamine KET and amphetamine AMP are often consumed by drug users at the same time, which seriously threatens people's health. Preparation of Au-XP Ag. Table 4 Comparison of ketamine and Amphetamine detection performances in different methods.

No Exit: China’s State Surveillance over People Who Use Drugs

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