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Identification of plasmalogens in Bifidobacterium longum . . .
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Changes in Phospholipid Composition of Erythrocyte . . .
Enzymatic measurement of ether phospholipids in human . . .
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Improvement of Blood Plasmalogens and Clinical Symptoms in . . .
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Tomomi Morisaki 1, Mika Okubo 1, Takako Emura 2 & Takehiko Fujino 1 . . . After being emulsified by an ultrasonic bath, the suspension was incubated at 45 °C for 60 min .
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Tomomi Morisaki Takehiko Fujino Plasmalogens are glycerophospholipids that contain a vinyl ether bond at the sn-1 position of glycerol backbone instead of an ester bond .
Strategy for enzymatic measurements of ether phospholipids in serum or plasma is showed in Fig . 1 .Phospholipase A 1 (PLA1) hydrolyzes diacyl phospholipids in plasma (serum) and leaves ePE and ePC intact . In the second step after lipid extraction, ePE is hydrolyzed by glycerophospholipid specific phospholipase D (GPL-PLD) to ethanolamine, and ePC is hydrolyzed by GPL-PLD to choline .
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The blood was cooled in an ice bath and kept in the refrigerator for 48 h at maximum . After centrifugation at 1000 ×g for 5 min at 4°C, plasma was kept at −30°C until measurement . . . . Ms . Seira Hazeyama, and Ms . Tomomi Morisaki, Institute of Rheological Functions of Food, for technical assistance with lipidomics . B&S Corporation Co . Ltd . . .
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