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Affiliation

1 ART Fertility Program of Alabama, Birmingham, AL 35209, USA. dbrownscience@ymail.com

David B Brown et al.

J Assist Reprod Genet .

2013 Apr .

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1 ART Fertility Program of Alabama, Birmingham, AL 35209, USA. dbrownscience@ymail.com

Phase contrast microscopic images of permeabilized fertile male sperm (negative control) at Time Zero, and sperm nuclei after a 5 min incubation in egg extract. All images are at the same magnification: Bar = 15 microns. Panel a Permeabilized negative control sperm from a fertile male at Time Zero, before mixing with egg extract. Panels b and c Typical examples of partially and fully decondensed negative control sperm nuclei after a 5 min incubation in egg extract
(Panels a-d): Microscopic images of a patient’s sperm with a positive (abnormal) SDAD Test score = 124.7 % of the negative control with fully decondensed sperm nuclei after a 5 min incubation in egg extract. All images are at the same magnification: Bar = 15 μm. Panel a : Microscopic images of 2 fully decondensed sperm nuclei by a phase dark sperm with no decondensation, and the sperm’s tail wrapped around the sperm head. Panels b and c : Typical images of hyper-decondensed sperm nuclei. Panel d : Clumped decondensed sperm nuclei, and variable hyper-decondensed sperm nuclei, all in the same field of view
Microscopic images of typical sperm nuclear decondensation observed for patients with normal, or abnormal SDD Test scores, after 15 min incubations in egg extract. All images are at the same magnification: Bar = 15 microns. Panel a : Permeabilized negative control sperm from a fertile male at Time 0, before mixing with egg extract. Panel b : Fully decondensed (FD) negative control sperm nuclei after a 15 min incubation in egg extract where 97.9 % of the sperm nuclei were fully decondensed. This is representative of the fully decondensed sperm nuclei observed when scoring patients’ found to have normal SDD Test scores. Panel c : Microscopic images of sperm from a patient with an SDD Test score = 62.0 % of the negative control with fully decondensed sperm nuclei after a 15 min incubation in egg extract. Thirty eight percent of this patient’s sperm nuclei had either no decondensation (ND) or partial decondensation (PD)
Pictorial representation of decondensed sperm nuclei of a patient with an abnormal SDAD Test score ≥ 120 % of the negative control (Fig. 2); with a normal SDD Test score ≥ 80 % of the negative control after a 15 min incubation in egg extract. Panels a and b : Fully decondensed sperm nuclei, and variable recondensation (99.7 % of the negative control). Panel c : Typical very hyper-decondensed sperm nucleus; such sperm nuclei were never observed to recondense. Note: these sperm are always very phase light with little contrast

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Purpose:

The objective of this study was to evaluate a panel of three sperm function tests; tests known to assess different aspects of sperm functionality and genomic integrity, the: 1) Sperm DNA Accelerated Decondensation (SDAD(TM)) Test, 2) Sperm DNA Decondensation (SDD(TM)) Test, and 3) Sperm Penetration Assay (SPA), determining if positive and negative test scores correlated with failed and successful ICSI outcomes, respectfully.

Methods:

A prospective, double blinded, cohort study was performed. One study sample (ejaculated semen) was collected by each of the 60 male partners of the 60 couples enrolled in the study; males whose female partners were found to have no major female factor issues. The sperm from each male was analyzed in the SPA, and SDAD and SDD Tests, and used for ICSI (1 ICSI cycle per couple).

Results:

The ICSI cycle pregnancy rate for this study was 50 %, with a delivery rate=40 % (n=60 ICSI cycles). The SPA and SDD Test scores did not significantly predict ICSI outcome when used as stand-alone tests (p>>0.05). However, when the SPA and SDD Test scores were used together, ICSI outcomes for a subgroup of 10 (16.7 %) males, were significantly predicted (p=0.03), with 1 live birth, and 9 negatives where the transferred embryos did not implant. In total, 38.4 % of the couples in this study were found to have a very poor chance for a successful ICSI cycle.

Conclusion:

SDAD Test scores alone, and SPA and SDD Test scores used together, significantly predicted failed ICSI outcomes. This indicates that the scores obtained when analyzing patients' sperm using a panel of sperm function tests; specifically, the SPA, and SDAD and SDD Tests, can be used to identify infertile couples who should not be directed to ICSI.

Phase contrast microscopic images of…
Phase contrast microscopic images of permeabilized fertile male sperm (negative control) at Time…
(Panels a-d): Microscopic images of…
(Panels a-d): Microscopic images of a patient’s sperm with a positive (abnormal) SDAD…
Microscopic images of typical sperm…
Microscopic images of typical sperm nuclear decondensation observed for patients with normal, or…
Pictorial representation of decondensed sperm…
Pictorial representation of decondensed sperm nuclei of a patient with an abnormal SDAD…

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J Exp Clin Assist Reprod

v.7; 2010

PMC2868308

Find articles by Stephen G. Somkuti
1 Abington IVF & Genetics, Abington Reproductive Medicine, Abington, PA, 19001, USA
2 Toll Center for Reproductive Sciences, Abington Memorial Hospital, Abington, PA, 19001, USA
1025 East Pleasant Ave, Glenside, PA 19038, USA. Email:
moc.oohay@2civerazalanelej
This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/2.0/ ).
Keywords: Hyaluronan-binding assay, sperm penetration assay, male partner screening
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