Sperm Mediated Gene Transfer Overview

Sperm Mediated Gene Transfer Overview
Sperm-mediated gene transfer (SMGT) is a transgenic technique that transfers genes based on the ability of sperm cells to spontaneously bind to and internalize exogenous DNA and transport it into an oocyte during fertilization to produce genetically modified animals. 1 Exogenous DNA refers to DNA that originates outside of the organism.
These include mice, pigs, fish, cattle, chicken, and Xenopus. There are three critical steps for successful sperm-mediated gene transfer to create transgenic animals. The first step is the binding of DNA on the sperm-cell surface, followed by the internalization of foreign DNA into the nucleus, and finally the integration of exogenous DNA.
In the last years, we have devoted much effort to clarify the mechanism of sperm/DNA interaction, with the dual aim to improve the protocols for transgenesis and to expand our understanding of the molecular mechanism (s) underlying sperm-mediated gene transfer.
Feb 15, 2024
The concept of Sperm-Mediated Gene Transfer (SMGT) describes the ability of spermatozoa to deliver to embryos not only their own genome during fertilization, but also foreign genetic information with which they may come in contact. The concept was established in 1989, when it was first shown that exogenous DNA incubated with mouse spermatozoa can be detected in tissues of born offspring. That ...
Abstract Sperm-mediated gene transfer (SMGT) is a procedure through which new genetic traits are introduced in animals by exploiting the ability of spermatozoa to take up exogenous DNA molecules and deliver them to oocytes at fertilization. The interaction of exogenous DNA with sperm cells is a regulated process mediated by specific factors; among those, a reverse transcriptase (RT) activity ...
Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing genetically modified animals with high efficiency.
It is reasonable to hypothesize that RNAs delivered by sperm cells at fertilization also exert these regulatory functions and remodulate the gene expression profile in early embryos. Consequently, new genetic circuits (canalized circuits) become functionally active, or/and pre-existing ones are rewired.
The sperm mediated gene transfer (SMGT), is a mass gene transfer method that uses the capability of spermatozoa in attracting exogenous DNA and transport it into an oocyte during natural or ...
Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem cells.Studies of sperm-mediated gene transfer (SMGT) indicate that sperm cells possess the ability to be utilized as carriers of exogenous ...
The opportunities stem from the fact that numerous different approaches have been developed for introducing transgenes into the sperm and testis, mainly because of the interest in using sperm mediated gene transfer and testis mediated gene transfer as ways to generate transgenic animals.
Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell- mediated production of Tg animals, sperm- mediated gene transfer (SMGT), and testis- mediated gene transfer (TMGT).
Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA ...
Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences. A substantive body of evidence indicates that SMGT is potentially useable in animal transgenesis, but also suggests that …
Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell-mediated production of Tg animals, sperm-mediated gene transfer (SMGT), and testis-mediated gene transfer (TMGT).
; Illustration of sperm mediated gene transfer in transgenic animals. Simple incubation of sperm cells with exogenous DNA may permit the DNA to be inserted into the sperm cell.
Abstract and Figures Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences.
Spermatozoa are highly specialized cells which play a central role in the process of fertilization, carrying the male genome into the oocyte. Although widely studied, the biological process of fertilization remains still obscure in many aspects, and the molecular...
The sperm-mediated gene transfer (SMGT) method developed by Lavitrano and collaborators, is based on the intrinsic ability of sperm cells to bind and internalize exogenous DNA and to transfer it into the egg at fertilization [10, 11, 12, 13, 14].
Lavitrano M et al (2002) Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation.
If sperm cells can act as vectors for exogenous DNA, it follows that the genome of sexually reproducing animals may be subject to alteration by exogenous DNA sequences carried by sperm cells. At present there are insufficient data to permit quantification of the rate of sperm-mediated gene transfer.
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described th …
Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem cells. Studies of sperm-mediated gene transfer (SMGT) indicate that sperm cells possess the ability to be utilized as carriers of exogenous ...
Exploiting this ability, protocols for sperm-mediated gene transfer have been developed in a variety of animal species with extremely variable results.
Abstract and Figures Sperm-mediated DNA transfer to offspring has the potential to markedly simplify the generation of transgenic animals, but the efficiency in mice has been controversial.
Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences. A substantive body o...
Download Citation | Methodology of sperm-mediated gene transfer | The capture of exogenous DNA and its transfer to eggs by sperm cells are the two key processes in SMGT. During the past two ...
Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing genetically modified animals with high efficiency.
Request PDF | Sperm-mediated gene transfer | Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the ...
The use of a sperm cell to introduce exogenous DNA into an oocyte at the time of fertilization is of interest for the simple production of transgenic mice, and is now called 'sperm‐mediated gene transfer (SMGT)'. In vivo transfection ...
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into
Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences ...
References (23) Abstract Sperm mediated gene transfer (SMGT) was developed as an alternative technique for the production of transgenic animals.
The implications of sperm-mediated gene transfer are significant and include evolutionary effects on the mammalian genome and pathologies in humans from de novo mutations. Despite the absence of firm data, geneticists would be wise to be vigilant to the potential consequences of sperm-mediated gene transfer.
Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem cells. Studies of sperm-mediated gene transfer (SMGT) indicate that sperm cells possess the ability to be utilized as carriers of exogenous ...
Sperm-mediated gene transfer (SMGT), including its alternative method, testis-mediated gene transfer (TMGT), becomes an established and reliable method for transgenesis.
The newly developed approach of FGCMGT, ovary-mediated gene transfer (OMGT) is also a novel and useful tool for efficient transgenesis. This review highlights an overview of the recent progress in germ cell mediated gene transfer techniques, methods developed and mechanisms of nucleic acid uptake by germ cells.
Testis-mediated gene transfer (TMGT) is a novel alternative variant of SMGT used to produce transgenic animals in vivo. This chapter presents the background and features of both non-viral and viral forms of SMGT, together with TMGT, as developed in recent years. Additionally, modes of fertilisation by transgene-bearing sperm are discussed.
Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences. A substantive body o...
In the mid-1970s, gene transfer with retroviruses, by early-1980s DNA microinjection and in 1982 production of foreign protein and growth hormone is possible. Later embryonic stem cell mediated gene transfer becomes perfected. For example, United Kingdom laboratories contain more than 575,000 transgenic mice in the year 2000.
The sperm-mediated gene transfer method potentially offers obvious advantages compared to the more widely used microinjection: it does not require sophisticated and expensive equipment such as micromanip ulators, can be easily performed in field experiments as it only requires very basic equipment, does not need any particular skill, and can be suc cessfully applied to those animal species ...
In the last years, we have devoted much effort to clarify the mechanism of sperm/DNA interaction, with the dual aim to improve the protocols for transgenesis and to expand our understanding of the molecular mechanism (s) underlying sperm-mediated gene transfer.
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation.
Sperm-mediated DNA transfer to offspring has the potential to markedly simplify the generation of transgenic animals, but the efficiency in mice has been controversial. To determine the basis of the variability of the procedure in mice, we undertook a large, collaborative study of sperm-mediated DNA …
Related collections and offers Overview Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem cells.
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This method known as sperm mediated gene transfer or SMGT. However, simple incubation of naked DNA with sperm head is not efficient enough to integrate the foreign DNA into the genome of the sperm.
Sperm-mediated gene transfer (SMGT), the ability of sperm cells to spontaneously incorporate exogenous DNA and to deliver it to oocytes during fertilization, has been proposed as an easy and efficient method for producing transgenic animals.
Sperm-mediated gene transfer (SMGT), including its alternative method, testis-mediated gene transfer (TMGT), becomes an established and reliable method for transgenesis.
PDF | This monograph describes the progress of studies on sperm as vectors for gene transfer for production of transgenic fish at the Fisheries Biology... | Find, read and cite all the research ...
Among transgenic techniques, we describe here the sperm-mediated gene transfer method that is based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to the oocyte during fertilization, producing genetically modified animals. Keywords: Mouse; SMGT; Spermatozoa; Transgenesis; Transgenic animals.
Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences. A substantive body o...
We have called this phenomenon sperm-mediated 'reverse' gene transfer (SMRGT). Thus, a RT-mediated machinery operates in sperm cells and is responsible for the genesis and non-Mendelian propagation of new genetic information.
Sperm Mediated Gene Transfer (SMGT) is based on the ability of sperm to bind, internalise, and transport exogenous DNA into an oocyte during fertilisation.
Abstract The ability to introduce controlled modifications of the genome of animals represents an important tool for biomedical and veterinary research. Among transgenic techniques, we describe here the sperm-mediated gene transfer method that is based on the … more
Apr 15, 2024
Jan 26, 2024
Here we consider new developments in both sperm and testis mediated gene transfer, including the use of viral vectors, as well as the technical and ethical challenges facing those who would seek to use these approaches for gene therapy as a way to treat male infertility. Keywords gene therapy, sperm, testis, transgene, viral vector
Sperm-Mediated Gene Transfer: Implications for Biotechnology and Medicine Page: 33-42 (10) Author: Michael Hölker, Nasser Ghanem, Dawit Tesfaye and Karl Schellander DOI: 10.2174/978160805237011201010033 PDF Price: $15 View Abstract
Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell- mediated production of Tg animals, sperm- mediated gene transfer (SMGT), and testis- mediated gene transfer (TMGT).
Sperm Mediated Gene Transfer: Concepts and Controversies includes essential background information for newcomers to the field, summarizes the work accomplished, and heralds the work to be done ...
Transgenic animals have been successfully produced by mass gene transfer techniques such as sperm-mediated gene transfer (SMGT). The aim of this work was to demonstrate transgene transmission by SMGT in chickens using dimethylsulfoxide (DMSO) or N,N-dimethylacetamide (DMAc) as transfectants after seminal plasma removal to prevent DNase activity. Sperm samples were prepared by repetitive washes ...
Sperm-mediated gene transfer (SMGT) is a procedure through which new genetic traits are introduced in animals by exploiting the ability of spermatozoa to take up exogenous DNA molecules and ...
Sperm-Mediated Gene Transfer: Implications for Biotechnology and Medicine Page: 33-42 (10) Author: Michael Hölker, Nasser Ghanem, Dawit Tesfaye and Karl Schellander PDF Price: $15 View Abstract
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