Sperm Extraction

Sperm Extraction

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Microscopic testicular sperm extraction (microTESE) is a procedure that takes sperm directly from the testicular tissue of a man’s reproductive system. If a man can't release or make enough healthy sperm naturally, this medical procedure may be recommended for fertility reasons (to enable the man to father a child).

American Society for Reproductive Medicine. Surgical Techniques for Sperm Retrieval: What Should I Know? (http://www.reproductivefacts.org/news-and-publications/patient-fact-sheets-and-booklets/fact-sheets-and-info-booklets/surgical-techniques-for-sperm-retrieval-what-should-i-know/) Accessed 5/6/2021.
American Urological Association. What is Sperm Retrieval? (https://www.urologyhealth.org/urologic-conditions/sperm-retrieval) Accessed 5/6/2021.
Spahovic H, Alic J, Göktolga Ü, et al. "Second-look" Micro Testicular Sperm Extraction (MicroTESE) in Patients with Non-obstructive Azoospermia Following Histopathological Analysis. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520053/) Med Arch . 2020;74(4):279-284. doi:10.5455/medarh.2020.74.279-284. Accessed 5/6/2021.

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Microscopic testicular sperm extraction (microTESE) is a procedure that takes sperm directly from the testicular tissue of a man’s reproductive system . If a man can't release or make enough healthy sperm naturally, this medical procedure may be recommended for fertility reasons (to enable the man to father a child). The testicular tissue is found in the two testes, where the sperm is made. The testes are found inside the scrotum, the small sac behind and under the penis.
The goals of the microTESE procedure are to:

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Infertility is a disease of the reproductive system that makes it difficult for the body to perform the basics of reproduction. It affects both men and women. Most infertility cases are treated with medication or surgery.
Male infertility in its most severe form may be caused by a condition known as nonobstructive azoospermia (the man does not produce sperm), or by obstructive azoospermia (sperm is produced, but blocked, and can't be released from the body).
Many times, the causes of infertility are unexplained. Sometimes, the difficulties may be from a genetic disease such as cystic fibrosis, a birth defect, a medical problem, or the result of an earlier treatment that may cause infertility (such as certain cancer treatments). In other cases, we do not know what the cause of the infertility is, but we still have treatments that may help.
If a couple is unable to conceive a child after several months of trying without birth control, they should see a doctor. If the woman is older than 35, the couple should see a doctor sooner. Your healthcare provider will perform a physical exam of both partners to check for any physical issues that may be causing infertility. The provider will also ask many questions and discuss each person's medical history.
The following tests may also be recommended or ordered to evaluate male fertility:
In-home testing kits may be available for semen analysis. Ask the doctor for more information.
Semen analysis will give the doctor necessary information to help assess fertility and create a treatment plan. The tests should show some of the following:
Microscopic testicular sperm extraction is usually performed on a man who is infertile and has no sperm in his ejaculation ( azoospermia ).
A urologist, a doctor who specializes in the male and female urinary tract and male reproductive organs, typically performs the procedure by making a few small cuts in the testicle and removing a small amount of tissue. Once the tissue is removed from the body, it is examined under a microscope to look for sperm. If found, the sperm can be used right away to fertilize an egg, or the sperm cells can be frozen, stored and then thawed at a later time to be used for infertility treatment.
The procedure is usually performed in the operating room with the patient under general anesthesia. A single incision is made in the middle of the scrotum. A microscope is used to help see the fine tubules of the testis that may contain sperm. All sutures (stitches) that are used dissolve on their own. The patient is able to go home the day of the procedure, and should be able to resume normal activities within one to two weeks.
Possible risks of the microTESE procedure include bleeding, infection, and pain or discomfort after the procedure. There is a very rare chance that the testicle itself will be damaged during the procedure.
When possible, any extra sperm retrieved during testicular sperm extraction should be frozen to preserve the sperm and avoid further procedures.
Although freezing the sperm may have some negative effects, medical experts believe that the outcome is generally the same as using non-frozen sperm in fertility procedures such as intra-cytoplasmic sperm injection (ICSI), a type of in vitro fertilization injecting one sperm directly into one egg. In vitro fertilization is a procedure in which a sperm and an egg are fertilized outside of the body, in a laboratory. The fertilized egg is then placed in the female’s uterus.
If the microTESE procedure needs to be repeated, it is usually best to wait six to 12 months.
Last reviewed by a Cleveland Clinic medical professional on 05/07/2021.

Cleveland Clinic is a non-profit academic medical center. Advertising on our site helps support our mission. We do not endorse non-Cleveland Clinic products or services.
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Understanding the Different Types of Sperm Extraction

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Sperm extraction is a way to overcome male factor infertility. In cases where there’s no sperm present in the ejaculate (azoospermia), our doctors at California Center for Reproductive Health use a variety of techniques to acquire sperm to use in IVF (in vitro fertilization) or ICSI (intracytoplasmic sperm injection).
Azoospermia may be due to a blockage in the male reproductive tract . You can produce sperm, but it can’t get into your semen to fertilize your partner’s egg. In nonobstructive azoospermia, you have little or no sperm production, usually due to defects in your testicles’ function or structure.
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