Snf Dissertation

Snf Dissertation




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"Functional and Structural Dissection of the SWI/SNF . . .
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"Transcriptional Regulation During Adipocyte . . .
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The yeast SWI/ SNF complex is the prototype of a subfamily of ATP-dependent chromatin remodeling complexes . It consists of eleven stoichiometric subunits including Swi2p/Snf2p, Swi1p, Snf5p, Swi3p, Swp82p, Swp73p, Arp7p, Arp9p, Snf6p, Snf11p, and Swp29p, with a molecular weight of 1 .14 mega Daltons . Swi2p/Snf2p, the catalytic subunit of SWI/ SNF , is evolutionally conserved from yeast to human cells .
Chromatin remodeling is crucial to transcriptional regulation in vivo and a number of protein complexes capable of altering genomic architecture in the budding yeast Saccaromyces cerevisiaehave been identified . Among these, the SWI/ SNF complex, a 2 MDa, eleven subunit protein assembly, has been the most extensively characterized . The SWI/ SNF complex is required for the proper expression of a . . .
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Chromatin has a compact organization in which most DNA sequences are structurally inaccessible and functionally inactive . Reconfiguration of thechromatir required to activate transcription . This reconfiguration is achieved by the action of enzymes that covalently modify nucleosomal core histones, and by enzymes that disrupt histone-DNA interactions via ATP hydrolysis . TheSWI/ SNF family of ATP . . .
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