Rotavirus PDF 86bc8f0b
ENGLISHremelxpectRotavirusINTENDEDUSERemelXpectRotavirusisarapidmembranebasedimmunogoldassayforthequalitativedetectionofrotavirusantigensinhumanfecalspecimensintendedforuseasanaidinthediagnosisofacutegastroenteritiscausedbyrotavirusinfectionsThistestisforprofessionaluseonlySUMMARYANDEXPLANATIONRotavirusisthemajorcauseoflifethreateningdiarrheagastroenteritisinchildrenyoungerthan2yearsRotavirusgastroenteritisisubiquitousanditoccursinallpartsoftheworldThesedoublestrandedRNAvirusescauseepidemicandendemicgastroenteritisingeriatricaswellaspediatricpatientsItafflictsmorethan130millioninfantsandchildrenannuallycausing1milliondeathsThevirusinfectstheepithelialcellsliningthesmallintestineAfteranincubationperiodof12daystheonsetofacutegastroenteritisissuddenwithvomitinganddiarrheafeveroccasionalabdominalpainandevenrespiratorysymptomsThedurationofthediseaseandthesymptomsmaylastbetween58daysThegoldstandardfordetectinghumanrotavirusdependsonelectronmicroscopysinceexistingtissueculturemethodsareunreliableXpectRotavirusoffersafastsimplemethodforthedetectionofrotavirusandrotavirusantigensPRINCIPLEXpectRotavirusutilizesapairofrotavirusspecificantibodiesinanimmunochromatographicsandwichassayAnextractisfirstpreparedbysuspensionofthespecimenintheprovidedextractionbuffersolutionThebuffercontainingtheextractedspecimenisthenaddedtothedevicesamplewellThereactionbetweenapositivesampleandthecoloredparticleconjugatedantibodywillformacomplexthatmigratesalongthemembraneAnimmobilizedcaptureantibodywillformacoloredlineattheSspecimenareauponreactingwiththecoloredcomplexAninternalcontrollineCcontrolareaisbuiltintoensurethatthetesthasbeenperformedcorrectlySTORAGEStoreXpectRotaviruskitatroomtemperatureuntiltheexpirationdateprintedontheboxThetestdevicemustremaininthesealedpouchuntilreadyforusePRECAUTIONS1ForInVitroDiagnosticUse2StandardprecautionsshouldbetakenagainstthedangersofbiologicalhazardsbyproperlysterilizingspecimenscontainersandtestdevicesafteruseConsultappropriatereferenceswhennecessaryDirectionsshouldbereadandfollowedcarefullyKitcomponentsandreagentsareprovidedatthenecessaryworkingstrengthdonotdiluteDonotinterchangereagentsbetweenkitsofdifferentlotsDonotusereagentsbeyondtheprintedexpirationdatesMicrobialcontaminationofreagentsmaydecreasetheaccuracyoftheassayandcausefalseresults8DonotreusekitcomponentsortestdevicesPoNoaSPECIMENCOLLECTIONSTORAGEANDTRANSPORTSpecimensshouldbecol
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