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The development of embryo sacs ES in vitro and induction of gynogenesis were studied in onion flower bud culture. Explants were divided into three groups according to their size at inoculation: a small flower buds 2. For histological study, excised ovaries were fixed at inoculation and then at 3-d intervals until day 12, and after 2 and 3 wk of culture. Some explants were cultured until embryo emergence, i. In total, ovules were examined histologically. In large flower buds, only mature ES occurred. In vitro conditions did not disturb meiosis and megagametophyte development in non-degenerated ovules. Regardless of the developmental stage at inoculation, only mature ES occurred on day Gynogenic embryos were found after 2 wk of culture, indicating that embryos developed in mature ES exclusively. Embryos were detected in 5. The parthenogenetic origin of the embryos is discussed. In addition, ES containing endosperm only 6. This is a preview of subscription content, log in via an institution to check access. Rent this article via DeepDyve. Institutional subscriptions. Bhojwani, S. In vitro gynogenesis. In: Bhojwani, S. Current trends in the embryology of angiosperms. Dordrecht: Kluwer Academic Publishers; — Google Scholar. Bohanec, B. Induction of gynogenesis in agricultural crops: a review. Variations in gynogenic response among long-day onion Allium cepa L. Plant Cell Rep. Genetic analysis of gynogenic haploid production in onion. Studies of gynogenesis in onion Allium cepa L. Plant Sci. Campion, B. Induction of haploid plants in onion Allium cepa L. Plant Cell Tiss. Organ Cult. Advances in haploid plant induction in onion Allium cepa L. Article Google Scholar. DeGroote, D. In vitro sexual and apomictic embryo sac development in Cenchrus ciliaris. Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber. Cucumis sativus L. Gu, Z. In vitro induction of haploid plantlets from unpollinated young ovaries of lily and its embryological observations. Acta Bot. Guha, S. In vitro development of ovule of Allium cepa L. Phytomorphology —; Huang, Q. Embryological observations on ovary culture of unpollinated young flowers of Hordeum vulgare. Chromosome doubling procedures of onion Allium cepa L. PubMed Google Scholar. Johri, B. Comparative embryology of angiosperms, Berlin: Springer-Verlag; — Jones, H. Development of the flower and macrogametophyte of Allium cepa. Hilgardia —; Kashin, A. Megagamete activation and regulation of in vitro embryogenesis in unpollinated millet ovaries. Plant Physiol. CAS Google Scholar. Keller, J. Culture of unpollinated ovules, ovaries, and flower buds in some species of genus Allium and haploid induction via gynogenesis in onion Allium cepa L. Euphytica —; Klein, M. Flower size and developmental stage of Allium cepa L. Acta Biol. Lobanova, L. The development of embryo sacs in in vitro ovaries of Nicotiana tabacum L. Michalik, B. Gynogenesis in Polish onion cultivars. Embryo sac development during the culture of placenta-attached ovules of Melandrium album. Embryological aspects of in vitro gynogenesis in plant organ cultures. Mukhambetzhanov, S. Culture of nonfertilized female gametophytes in vitro. Muren, R. Haploid plant induction from unpollinated ovaries in onion. HortScience —; Musial, K. Embryological study on gynogenesis in onion Allium cepa L. Plant Reprod. Influence of irradiated pollen on embryo and endosperm development in kiwifruit. Endosperm response to pollen irradiation in kiwifruit. Smith, B. Gynogenesis from whole flower buds in bulb onions Allium cepa L. Wu, B. Cytological and embryological studies on haploid and diploid plant production from cultured unpollinated ovaries of Nicotiana tabacum and Nicotiana rustica. In: Fujiwara, A. Plant Tissue, Cell Cult. Tokyo; — Yang, H. In vitro induction of haploid plants from unpollinated ovaries and ovules. In Bhojwani, S. Developments in crop science Plant tissue culture: applications and limitations. Amsterdam: Elsevier; — Zhou, C. In vitro culture of unpollinated ovaries in Oryza sativa L. In: Hu, H. Haploids of higher plants in vitro. Beijing: Springer-Verlag; — Download references. Grodzka 52, , Cracow, Poland. You can also search for this author in PubMed Google Scholar. Correspondence to Krystyna Musial. Reprints and permissions. The development of onion Allium cepa L. Embryo sacs in vitro and gynogenesis induction in relation to flower size. In Vitro Cell. Download citation. Received : 19 June Accepted : 10 February Issue Date : July Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Embryo sacs in vitro and gynogenesis induction in relation to flower size Published: July Volume 41 , pages —, Cite this article. Summary The development of embryo sacs ES in vitro and induction of gynogenesis were studied in onion flower bud culture. Access this article Log in via an institution. Somatic embryogenesis in ferns: a new experimental system Article Open access 20 January Somatic Embryogenesis of Immature Cunninghamia lanceolata Lamb. References Bhojwani, S. Google Scholar Bohanec, B. Google Scholar Guha, S. Google Scholar Huang, Q. Google Scholar Jones, H. Google Scholar Kashin, A. Google Scholar Klein, M. Google Scholar Lobanova, L. Google Scholar Mukhambetzhanov, S. Article Google Scholar Muren, R. Google Scholar Musial, K. Article Google Scholar Musial, K. Article Google Scholar Smith, B. Google Scholar Wu, B. Article Google Scholar Yang, H. Google Scholar Zhou, C. Google Scholar Download references. View author publications. Rights and permissions Reprints and permissions. About this article Cite this article Musial, K. Copy to clipboard. Search Search by keyword or author Search. Navigation Find a journal Publish with us Track your research.
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