Read the tutorial - Gene PDF 26efe505
OPCRTutorialVersion30CreateDateSep102008LastmodifiedMay252009byStephanPabingerPCRTutorialPCRTableofcontentTableoiConenaaeanio2IIOe1ROOEEENOREOEE311OE32SaveandEportAeSici321SSPPAENEN32HERREREERREtatpmEEA23SMA62DamOPERandoetNooeii64UploadTIESrtAd6YPierSEUSaee7OEETeTPasia81PietandanaZenOOSEEEeGeIeNee10DAaEOREOORREEENR1181IEAEOEAOEEEEOE1182MONAOidooooNOE1183to12NORRO13OOPIPENNE1391TETEooi13AEOA1493EEEEEEREEOREEEOEAEEE1510EgAalyzeResulta1611CAIUSa17UEiidiiTa0REEEREEEEe17EZ1DSEEiaa17HoLESPO1812SSeMEMeroetateee1913ADAHTH20131EgCalculationMethodsaseSIGFeGEdeRRIEAROeNDeRo20152SampleReaaE21OoARletfeiERGEESEADEseMecE21ASFeamEESAAenONANER2214AnalysisTESSAaT23ELLEE23142MultipleTiareisnacEESODEERNREGEDEReGeid25145Singleatole24144QualityConlara2315StatisticaLostEON26ENOOO26H221EEEEEREEEEEEDOEET2716EXPOeNaiiiii30Page230StephanPabingerPCRTutorialPCR1IntroductionOPCRisawebapplicationdesignedforstoringparsingmanagingandanalyzingqpCRdataIncludingseveraldifferentalgorithmsitcanfacilitatetheanalysisofqPCRresults11PurposeThistutorial19writtentogiveyouanexampleofhowtousethepresentedapplicationItshowsthetypicalanalysispathwhichstartswiththeexportoffilesandendswithprintreadychartsForthistutorialfilesoftwodifferentvendorsareusedeAppliedBiosystemsAbiPrismSDS7000abbreviatedasSDSeRocheLightcyclerLC405abbreviatedasLCBothfilesandthegeneratedrunsandexperimentsareavailableintheQPCRapplicationifyouloginasuserguestpasswordguestMoreoveryoucandownloadthemfromhttpsrtpcrgenometugrazatrtpcrinfoinfoindexhtml2SaveandExportFilesThefirststepintheanalysispipelineistocreatethefilesthatshouldbeanalyzedlateron21SDSAfterperformingtheqPCRexperimentthefileneedstobesavedGotofilegtsaveandsavethesdsfileABIPrism7000SDSSoftwareFile1sdsByFileViewToolsInstrumentAnalysisWindowHelpBE22xFeFile1sdssample4sample4MyRecentDocumentsDesktopsample4sample4MyDocumentsttsample4sample4grMyComputerysample4sample4mmoMEENFilenameEievPlacesSaveastypeSDSDocumentssdsyCancel4sample4sample4samplesample2sample3sample3sample4sample4samplesamplesample2sample2sample3sample3sample4sample4Hsample1samplesample2sample2sample3sample3sample4sample4
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