Quantum Blue C-Reactive Protein (CRP) PDF eb3d2e3a
Quantum Blue C-Reactive Protein (CRP)
PABHLMANNQuantumBlueCReactiveProteinCRPQuantitativeLateralFlowAssayLFCRP2525testsRevisiondate20130729BHLMANNLABORATORIESAGBaselst55Englishpage2aselstrasseCH4124SchnenbuchSwitzerlanduSeite2Tel41614871212rangaispageFax441614871234Italianopagina10infobuhlmannlabschEspanolpgina12ENGLISHINTENDEDUSETheQuantumBlueCReactiveProteinCRPLateralFlowAssayisasandwichtypeimmunoassaydesignedforthequantitativedeterminationofCRPinhumanserumincombinationwiththeBHLMANNQuantumBlueReaderPRINCIPLEOFTHEASSAYThetestisdesignedfortheselectivemeasurementofCRPantigenbysandwichimmunoassayAmonoclonalcaptureantibodymAbwhichishighlyspecificforCRPislinedontothetestmembraneAsecondmonoclonaldetectionantibodyconjugatedtogoldcolloidsisdepositedintheconjugatereleasepadandreleasedintothereactionsystemafteradditionofthetestsampleTheCRPantiCRPgoldconjugatebindstotheantiCRPantibodycoatedontothetestmembranetestlinetestbandandtheremainingfreeantiCRPgoldconjugatebindstothegoatantimouseantibodycoatedontothetestmembranecontrollinecontrolbandThesignalintensitiesofthetestlineandthecontrollinerespectivelyaremeasuredquantitativelybytheBHLMANNQuantumBlueReaderREAGENTSSUPPLIEDANDPREPARATIONeLetthereagentsadjusttoreachroomtemperatureMixwellvortexthereagentsbeforeuseeCartridgescannotbereusedeTestresultsshouldbeinterpretedinconjunctionwithinformationavailablefromclinicalassessmentofthepatientandotherdiagnosticproceduresSPECIMENCOLLECTIONANDSTORAGELessthan50ulofserumisrequiredCollectbloodintoplainvenipuncturetubesforSerumavoidhemolysismixbyinvertingsampletubeseveraltimesandleavetoclotfor45minutesatroomtemperature1828CprotectedfromlightCentrifugeat1800xgfor15minutesatroomtemperature1828CandcollecttheserumDonotheatinactivatesamplesSerumsamplescanbestoredrefrigeratedat28Cforupto7daysForlongerstoragekeepserumsamplesat20CThesamplesarestableforatleast4monthsNoteTheuseoflipaemichemolyticandictericsampleshastobevalidatedforthisassayLipaemicsamplescanbeavoidedbyaskingpatientstofastforatleast12hourspriortothesamplebeingtakenReagentsQuantityCodeCommentsTestCartridgevacuumsealedinafoil25piecesBLFCRPTCbag1vialChaseBuffer10mlBLFCRPCBReadytouse4TobeusedwithRFIDChipCardred1pieceBLFCRPRCCRFirmware104xTobeusedwithRFIDChipCardwhite1pieceBLFCRPRCCFirmware2217Table1STORAGEANDSHELFLIFEOFREAGENTSAllkitcomponen
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