Protocol - Geneflow Home - Molecular Biology Product Suppliers PDF 1c787b39
Protocol - Geneflow Home - Molecular Biology Product Suppliers
3430SchmonParkwayThoroldONCanadaL2V4Y6Phone8666674362e9052278848Fax9052271061EmailtechsupportnorgenbiotekcomBIOTEKwiCORPORATIONS2NORGENPPlasmaSerumRNAPurificationKitsProductInsertProduct550005610056200CellfreecirculatingRNAincludingexosomalRNAinplasmaorserumhasthepotentialtoprovidebiomarkersforcertaincancersanddiseasestatesandincludestumorspecificextracellularRNAinthebloodExosomesare40100nmmembranevesicleswhicharesecretedbymostcelltypesExosomescanbefoundinsalivabloodurineamnioticfluidandmalignantasciticfluidsamongotherbiologicalfluidsEvidencehasbeenaccumulatingrecentlythatthesevesiclesactascellularmessengersconveyinginformationtodistantcellsandtissueswithinthebodyTheexosomescontaincellspecificproteinslipidsandRNAswhicharetransportedtoothercellswheretheycanalterfunctionandorphysiologyTheseexosomesmayplayafunctionalroleinmediatingadaptiveimmuneresponsestoinfectiousagentsandtumourstissuerepairneuralcommunicationandtransferofpathogenicproteinsRecentworkhasdemonstratedthepresenceofdistinctsubsetsofmicroRNAswithinexosomeswhichdependuponthetumourcelltypefromwhichtheyaresecretedForthisreasonexosomalRNAsmayserveasbiomarkersforvariousdiseasesincludingcancerAstheRNAmoleculesencapsulatedwithinexosomesareprotectedfromdegradationbyRNAsestheycanbeefficientlyrecoveredfrombiologicalfluidssuchasplasmaorserumNorgensPlasmaSerumRNAPurificationKitsprovideafastreliablereproducibleandsimpleprocedureforisolatingcirculatingRNAandexosomalRNAfromsmallplasmaseruminputsrangingfrom50uLandupto5mLwithvariouskitformatsaddressingdifferentplasmaseruminputvolumesPurificationisbasedonspincolumnchromatographythatusesNorgensproprietaryresinseparationmatrixThekitisdesignedtoisolateallsizesofcirculatingRNAincludingmicroRNAaswellasallsizesofexosomalRNANorgensPlasmaSerumRNAPurificationKitsprovideaclearadvantageoverotheravailablekitsinthattheydonotrequirephenolchloroformoranyproteasetreatmentsRNAcanbeisolatedfromeitherfreshorfrozensamplesusingthesekitsMoreoverthekitsallowtheusertoeluteintoaflexibleelutionvolumerangingfrom10uLto100uLTypicalyieldsoffreecirculatingandexosomalRNAvarydependingontheinputsampleastheamountofRNApresentinplasmaandserumwilldependuponthehealthstatusoftheindividualNormallytheRNAyieldfromplasmaorserumRNAishighlyvariablerangingfrom1to100ngmLVariabilityisalsoobservedbetweensamplescollectedfromthesamedonoratdifferenttimesduringthedayThiskitissuitablefortheisolationofRNAfromfreshorfrozenserumorplas
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