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Jul 22, · To create the RosaCas9 knock-in allele, the RosaLSL-Cas9 targeting vector was used. This vector was designed with (from 5' to 3') a 5' homology arm, a ubiquitously expressed CAG promoter, loxP-flanked 3xSV40 polyA stop cassette, a 3X-FLAG epitope tag, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) Allele Name: targeted mutation , Feng Zhang.
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Tg(C9orf72_3) line mice have several tandem copies of the C9orf72_3 transgene, with each copy encoding the human C9orf72 with a hexanucleotide repeat expansion in the intron between the alternatively-spliced non-coding first exons 1a and 1b. Each transgene copy has between repeats ([GGGGCC] ). Hemizygous mice exhibit RNA foci and poly(GP) dipeptides in most .
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[HOST]-Tg (APPSwFlLon,PSEN1*ML*LV)Vas. behavior/neurological phenotype. decreased anxiety-related response. increased open to total arm entry ratio in 5 month old females, however, phenotype is more severe in homozygotes.
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New products arrive daily so keep checking back to see when this product becomes available. Please enter your zip code. Item # JAXF Number Available: 0. Your Zip Code: Not your Zip code? This item is currently out of stock. Item #: JAXF UPC: UPC Code: Description: BTA APX 9MM B PST 17RD: Brand.
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A transgene was designed with a mutant human amyloid beta (A4) precursor protein (APP) cDNA sequence (altered to include the APP KN/ML (Swedish) + IV (Florida) + VI (London) Familial Alzheimer's Disease (FAD) mutations) inserted into exon 2 of the mouse Thy1 gene.A second transgene was designed with a mutant human presenilin 1 (Alzheimer disease 3) (PSEN1 or PS1) .
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