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Prehybridization (Blocking): Wash the nylon membrane with a prehybridization solution containing salmon sperm DNA to block non-specific DNA interactions and reduce background noise . Alternatively, use the PerfectHyb™ Plus buffer, which doesn't require salmon sperm DNA for blocking .
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Semen normally smells like ammonia, bleach, or chlorine . Semen is about 1 percent sperm and 99 percent other compounds, enzymes, proteins, and minerals . . Many of these substances are alkaline . . .
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kelleycake: I used to spend time after school and on many weekends at my Aunt's home because I had no dad and my mom worked full time . My mom and Aunt knew I had female tendancies early on but I'll never forget the first time I got caught in the act . It was late at night and I thought my Aunt was asleep . During that day I stashed the perfect outfit to play with at night .
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