Molecular investigations on archival tissues

Molecular investigations on archival tissues

👓 Jorunn Litlekalsoy
Molecular investigations on archival tissues

Molecular investigations on archival tissues

✅ Nowadays there are many available molecular methods for studying diseases. Archival tissue provides an extremely valuable material for proper understanding of pathology of various diseases. A major problem in molecular analysis of archival tissues is the tissue degradation that might occur during long storage. This monograph presents various methods for efficient extraction of DNA and protocols for sequencing (detection of gene mutations) from archival tissues. It also provides protocols for immunodetection of various proteins on such archival tissues collected at Gades Institute, Haukeland University Hospital, Norway in the period 1932 till 1996. In addition this book illustrates the value of studies on archival tissues for detecting changes in the pathogenesis of diseases with time. In this monograph bladder cancer is used as an example for such studies. This book could be useful for professionals, considering the use of archival tissue to study mechanisms of various diseases.



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