Methyl Red Test Lab Report

Methyl Red Test Lab Report




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Methyl Red (MR) test is a biochemical test performed on bacterial species to detect the ability of an organism to produce stable acids end products (Mixed-acid fermentation) from supplied glucose.It is used as the part of the IMViC tests, a set of four useful reactions that are commonly designed for the differentiation of enterics (members of family Enterobacteriaceae).

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The pH at which methyl red detects acid is considerably lower than the pH for other indicators used in bacteriologic culture media. Thus, to produce a color change, the test organism must produce large quantities of acid from the carbohydrate substrate being used. MR Positive: When the culture medium turns red after the addition of methyl red ...

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Figure 1. FIG. 1. A positive methyl red test on Escherichia coli (left) and a negative methyl red test on Enterobacter aerogenes (right). The positive result given by Escherichia coli is indicated by the red color after the addition of methyl red reagent. The negative result given by Enterobacter aerogenes is indicated by the lack of change after the addition of methyl red.


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The methyl red (MR) test detects the production of sufficient acid during the fermentation of glucose and the maintenance of conditions such that the pH of an old culture is sustained below a value of about 4.5, as shown by a change in the colour of the methyl red indicator which is added at the end of the period of incubation.