Mature 3

Mature 3
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Affiliation

1 Research Institute of Molecular Pathology, Vienna, Austria.

R Eckner et al.

EMBO J .

1991 Nov .

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1 Research Institute of Molecular Pathology, Vienna, Austria.

Dominski Z, Sumerel J, Hanson RJ, Whitfield ML, Marzluff WF.
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We have analysed nucleocytoplasmic export of mRNAs in primate cells with the aim of identifying signals promoting RNA transport. Our results demonstrate that sequences directing either histone mRNA 3' processing or cleavage/polyadenylation of mRNA stimulate nucleocytoplasmic RNA transport. To elucidate the nature of this stimulation, we engineered test gene transcripts which could obtain a mature histone 3' end by the RNA cleaving activity of a cis-acting ribozyme, thus circumventing the cellular 3' end processing machinery. However, such transcripts were found to be transport deficient and accumulated in the nuclear compartment. Our experiments provide genetic evidence that there is a linkage between 3' end formation and the export of RNA transcripts from the nucleus. Analysis of a similar series of histone mRNAs in which the mature 3' end was generated by means of ribozyme cleavage led to the discovery of a second export mechanism which relies on features specific for mature histone RNA and which can be uncoupled from the cellular processing machinery. The presence of histone mRNA sequences and of the highly conserved histone hairpin structure, positioned close to the 3' terminus, are critical determinants for this export mechanism.

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https://youtu.be/gaXJq5E8-m8 had no idea my regular room was going to be upgraded to a suite so i called my friend to come and NJOY it with me !!! what a night....

Practice What You Preach (Lp Version)

584,070 views Aug 28, 2018 https://youtu.be/gaXJq5E8-m8 had no idea my regular room was going to be upgraded to a suite so i called my friend to come and NJOY it with me !!! what a night.... … ...more

Practice What You Preach (Lp Version)

Practice What You Preach (Lp Version)

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0:46 / 9:08 • Watch full video Live

https://youtu.be/gaXJq5E8-m8 had no idea my regular room was going to be upgraded to a suite so i called my friend to come and NJOY it with me !!! what a night....

Practice What You Preach (Lp Version)

584,070 views Aug 28, 2018 https://youtu.be/gaXJq5E8-m8 had no idea my regular room was going to be upgraded to a suite so i called my friend to come and NJOY it with me !!! what a night.... … ...more

Practice What You Preach (Lp Version)

Practice What You Preach (Lp Version)

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