Manual - RayBiotech 04d0b7b4
Inc. PDF ,
RayBioCSeriesHumanCytokineAntibodyArrayC11Forthesemiquantitativedetectionof37humancytokinesinserumplasmacellculturemediaandotherliquidsamplestypesPatentPendingTechnologyUserManualRevisedOctober282014CatAAHCYT1122SampleKitCatAAHCYT1144SampleKitCatAAHCYT1188SampleKitPleasereadmanualcarefullybeforestartingexperimentRayBiotechInctitheproteinarraypioneercompanyTelTollFree18884948555or17707292992Fax17702062393WebsitewwwraybiotechcomEmailinforaybiotechcomViVIIVIIIxIXIICSeriesTABLEAntibodyArraysTABLEOFCONTENTSIntroductionComponentsandStorageAdditionalMaterialsRequiredSampleTipsandGeneralConsiderationsASampleCollectionPreparationandStorageBSampleTypesandRecommendedDilutionsAmountsCHandlingMembranesDIncubationsandWashesChemiluminescenceDetectionTipsComponentPreparatioProtocolABlockingBSampleIncubationCFirstWashDBiotinylatedAntibodyCocktailIncubationESecondWashFHRPStreptavidinIncGThirdWashnubationStorageTypicalResultsAControlSpotsBDataExtractionCDataAnalysisArrayMapTroubleshootingGuideInterpretingtheResultsOWWOWOWOONNDADAMHPNPRPPRPPRPRPRPPPPROBNNNNFPFOOOOlINTRODUCTIONNewtechniquessuchascDNAmicroarrayshaveenabledustoanalyzeglobalgeneexpressionHoweveralmostallcellfunctionsareexecutedbyproteinswhichcannotbestudiedsimplythroughDNAandRNAtechniquesExperimentalanalysisclearlyshowsdisparitycanexistbetweentherelativeexpressionlevelsofmRNAandtheircorrespondingproteinsThereforeanalysisoftheproteomicprofileiscriticalTheconventionalapproachtoanalyzingmultipleproteinexpressionlevelshasbeentouse2DSDSPAGEcoupledwithmassspectrometryHoweverthesemethodsareslowexpensivelaborintensiveandrequirespecializedequipmentThuseffectivestudyofmultipleproteinexpressionlevelscanbecomplicatedcostlyandtimeconsumingMoreoverthesetraditionalmethodsofproteomicsarenotsensitiveenoughtodetectmostcytokinestypicallyatpgmlconcentrationsCytokinesbroadlydefinedassecretedcellcellsignalingproteinsdistinctfromclassichormonesorneurotransmittersplayimportantrolesininflammationinnateimmunityapoptosisangiogenesiscellgrowthanddifferentiationTheyareinvolvedinmostdiseaseprocessesincludingcancerobesityandinflammatoryandcardiacdiseasesSimultaneousdetectionofmultiplecytokinesundoubtedlyprovidesapowerfultooltostudycellsignalingpathwaysRegulationofcellularprocessesbycytokinesisacomplexdynamicprocessofteninvolvingmultipleproteinsPositiveand
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