Madeira Hydroponics
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Aquaponics and circular economy in Madeira Island: A Case Study of the Islandap Project
Madeira, T. Drake, Julian K. Rhizosecretion of recombinant pharmaceuticals from in vitro hydroponic transgenic plant cultures is a simple, low cost, reproducible and controllable production method. Here, we demonstrate the application and adaptation of this manufacturing platform to a human antivitronectin IgG 1 monoclonal antibody mAb called M The rhizosecretome was determined to contain proteins, with the mAb heavy and light chains the most abundant. This enabled evaluation of a simple, scalable extraction and purification protocol and demonstration that only minimal processing was necessary prior to protein A affinity chromatography. The binding of M12 purified from hydroponic medium to vitronectin was comparable to its Chinese hamster ovary CHO -derived counterpart. This study demonstrates that in vitro hydroponic cultivation coupled with recombinant protein rhizosecretion can be a practical, low-cost production platform for monoclonal antibodies. High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures. T1 - High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures. N2 - Rhizosecretion of recombinant pharmaceuticals from in vitro hydroponic transgenic plant cultures is a simple, low cost, reproducible and controllable production method. Here, we demonstrate the application and adaptation of this manufacturing platform to a human antivitronectin IgG1 monoclonal antibody mAb called M AB - Rhizosecretion of recombinant pharmaceuticals from in vitro hydroponic transgenic plant cultures is a simple, low cost, reproducible and controllable production method. Overview Fingerprint. Abstract Rhizosecretion of recombinant pharmaceuticals from in vitro hydroponic transgenic plant cultures is a simple, low cost, reproducible and controllable production method. Keywords Genetically modified plants Monoclonal antibody Recombinant antibody production Rhizosecretion. Access to Document Plant Biotechnology Journal , 14 2 , Madeira, L. In: Plant Biotechnology Journal. Madeira and T. In: Plant Biotechnology Journal , Vol. AU - Szeto, T. AU - Ma, Julian K. PY - Y1 - N2 - Rhizosecretion of recombinant pharmaceuticals from in vitro hydroponic transgenic plant cultures is a simple, low cost, reproducible and controllable production method. Plant Biotechnology Journal.
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High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures
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Aquaponics and circular economy in Madeira Island: A Case Study of the Islandap Project
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