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Karl Fischer titration – Frequently Asked Questions | Metrohm

Войдите, чтобы увидеть цены контракта. Посмотреть корзину. Automation is often thought of only as a solution when moving into high-throughput applications or when repeatedly running fixed workflows with no variability. However, there are many workflows that vary from run to run that would benefit from automation due to factors such as complexity, high error rates, and ensuring data integrity. A common and seemingly simple example of this workflow is hit picking. The basic concept of hit picking is simple — 1 identify hits based on assay data and 2 consolidate these hits into a new labware in an identifiable way. However, there are numerous areas where things can go wrong when performing this task at the bench. First, analyzer data must mapped back to the correct source wells and the wells containing hits must be identified. These wells must then be transferred in a consistent order to ensure no hits are mislocated. Despite all of these potential sources of error, there is little in the way of safeguards when performing this task manually. Here we will describe how hit picking was automated as part of a cell line development workflow. This lengthy and complex workflow had multiple hit picking steps. Figure 1. Hit identification. B Cells were grown for three weeks and wells with colony growth were identified by imaging. We utilized the Span-8 pod of a Biomek i7 Figure 2A to rapidly sample the media from the primary hit wells across four well plates. The transfers were directly driven by the imported confluence data and the spanning capability enabled the simultaneous sampling of multiple spaced wells per column to accelerate the process Figure 2B. In addition, the fine level of pipetting control allowed slow aspirate speeds and optimal tip location to avoid disrupting the loosely adherent cells. The Span-8 pod dispensed replicate wells for each primary hit well into a single black well plate — a challenging labware to execute a reformatting process with manually. Figure 2. Automated hit picking. As challenging as the physical aspects of hit picking are, ensuring data integrity during a reformatting process is just as essential. In this example, data that is generated on the well assay plate must be correctly attributed to the source wells to drive a second hit picking step. During liquid transfers, Biomek can automatically transfer the source well information e. Figure 3. Data integrity during reformatting. A DART software ensures data integrity by automating the transfer of data from the reformatted assay plate back to the original source wells. B The centralized data can be accessed through reports shown , web browsers, and used by the automation for additional data-driven hit picking steps. While just a small part of a larger cell line development workflow, the hit picking steps would likely be the most error prone when manipulating both samples and data. We have shown how automating both the physical transfers and data handling aspects of these reformatting steps enable a rapid and error free hit picking process. Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions. Beckman Coulter Life Sciences genomic reagent kits are for research use only. Download PDF. Узнать больше. Свяжитесь с нами. Войдите, чтобы увидеть цены контракта Your shopping cart is empty. Количество Цена Посмотреть корзину. Добавлено в корзину Посмотреть корзину. Войти Создать учетную запись Мой Аккаунт Просмотр и отслеживание покупок. Выберите страну Выберите язык Изменить. Cell Line Development — Hit Picking. Content Type: Application Note Summary Hit picking is a frequent need in many workflows, including cell line development. Заполните форму ниже, чтобы связаться с нами. К сожалению, страница, которую вы хотели посмотреть, не найдена. Вместо этого мы нашли что-то похожее. Если это не то, что вам нужно, воспользуйтесь поиском.

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