Isolation and ➕ molecular characterization of dye tolerant bacteria 🦠 from dye contaminated soil in Kerala

Isolation and ➕ molecular characterization of dye tolerant bacteria 🦠 from dye contaminated soil in Kerala

👓 Jiby John Mathew,Prem Jose Vazhacharickal,Sajeshkumar N.K
Isolation and ➕ molecular characterization of dye tolerant bacteria 🦠 from dye contaminated soil in Kerala

Isolation and ➕ molecular characterization of dye tolerant bacteria 🦠 from dye contaminated soil in Kerala

✅ Scientific Study from the year 2️⃣0️⃣1️⃣7️⃣ in the subject Biology 🧫 - Micro- and ➕ Molecular Biology 🧫, grade: 1️⃣.5️⃣, Mar Augusthinose College, language: English 🏴󠁧󠁢󠁥󠁮󠁧󠁿, abstract: The dyes are complex chemical compounds that imparts colour to substances. On the urge of urbanisation, the use of synthetic dyes is increasing largely and ➕ its untreated effluent release causes serious environmental pollution affecting water 🌊 bodies by disturbing the aquatic ecosystem, soil, plants 🌵, animals, humans. The recalcitrant nature of these dyes limits its treatment using conventional methods wherein biological methods using microorganisms are reported to completely mineralize the dyes lowering the release of degradation products. The present 🎁 work ⚙️ focuses on the biodegradation the biodegradation of synthetic dyes by isolating bacteria 🦠 from a dyeing unit. Soil samples from the dye contaminated soils was collected, its degradation potentiality was collected, its degradation potentiality was observed using three 3️⃣ major dye of studies congo red 🔴, brilliant green 💚 and ➕ methylene blue 🔵 within 2️⃣4️⃣ hr incubation. Maximum decoloarized dye (congo red 🔴 and ➕ brilliant green 💚) were chosen, serially diluted to 10 🔟-5️⃣ and ➕ plated 🍽️ to obtain two 2️⃣ distinct colonies from decolorized congo red 🔴 (CR1️⃣, CR2️⃣) and ➕ three 3️⃣ distinct colonies from decolorized brilliant green 💚 (BG1️⃣, BG2️⃣, BG3️⃣). These isolates were biochemically characterized. Molecular characterization was performed 🎭️ by isolating DNA 🧬 from five 5️⃣ isolates and ➕ amplified it using PCR, with the 1️⃣6️⃣s rRNA gene 🧬 primer. The PCR amplification product having approxima...



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