Галерея 3114848

Галерея 3114848




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Галерея 3114848


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Dubey JP, Andrews CD, Lind P, Kwok OC, Thulliez P, Lunney JK.
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Three four-week-old calves were used in a study on serological responses in combined Sarcocystis cruzi and Toxoplasma gondii infections. One calf (C1) was inoculated orally with 100,000 S cruzi sporocysts, another (C2) with 40,000 sporocysts, while the third (C3) acted as uninfected control. One hundred and five days later, all three calves were inoculated orally with 100,000 T gondii oocysts. The antibody responses to sarcocystis and toxoplasma during 210 days were recorded, using different serological assays. During the S cruzi monoinfection phase, positive antibody reactions were recorded in C1 and C2, using an ELISA with soluble (cytoplasmic) T gondii tachyzoite antigen, while antibody reactions remained negative in the toxoplasma indirect fluorescent antibody test and in an ELISA employing T gondii tachyzoite cell membrane antigen. However, the Sabin Feldman dye test also showed transient low positive titres for C1 and C2 at around day 39 after S cruzi inoculation. When using an ELISA employing soluble S fusiformis cystozoite antigen, increased sarcocystis antibody counts were recorded for C1 and C2 starting two weeks after T gondii inoculation. Otherwise the three calves reacted as expected regarding the general antibody responses to the S cruzi and T gondii infections.


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Published online 2011 Jun 14. doi: 10.1371/journal.pone.0020899

Department of Hematology and Medical Oncology, Emory University School of Medicine and Winship Cancer Institute, Atlanta, Georgia, United States of America

Wayne State University, United States of America
Received 2011 Mar 31; Accepted 2011 May 12.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
GUID: 5CE97979-5B88-4A17-A97E-8A2DBD13372E
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Articles from PLOS ONE are provided here courtesy of PLOS
1. Shaw RJ, Bardeesy N, Manning BD, Lopez L, Kosmatka M, et al. The LKB1 tumorsuppressor negatively regulates mTOR signaling. Cancer Cell. 2004; 6 :91–99. [ PubMed ] [ Google Scholar ] [ Ref list ]
2. Makowski L, Hayes DN. Role of LKB1 in lung cancer development. Br J Cancer. 2008; 99 :683–688. [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
3. Ding L, Getz G, Wheeler DA, Mardis ER, McLellan MD, et al. Somatic mutations affect key pathways in lung adenocarcinoma. Nature. 2008; 455 :1069–1075. [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
4. Motzer RJ, Escudier B, Oudard S, Hutson TE, Porta C, et al. Efficacy of everolimus in advanced renal cell carcinoma: a double-blind, randomised, placebo-controlled phase III trial. Lancet. 2008; 372 :449–456. [ PubMed ] [ Google Scholar ] [ Ref list ]
5. Abraham RT, Gibbons JJ. The mammalian target of rapamycin signaling pathway: twists and turns in the road to cancer therapy. Clin Cancer Res. 2007; 13 :3109–3114. [ PubMed ] [ Google Scholar ] [ Ref list ]
6. Maira SM, Stauffer F, Brueggen J, Furet P, Schnell C, et al. Identification and characterization of NVP-BEZ235, a new orally available dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor with potent in vivo antitumor activity. Mol Cancer Ther. 2008; 7 :1851–1863. [ PubMed ] [ Google Scholar ] [ Ref list ]
7. Konstantinidou G, Bey EA, Rabellino A, S
Худая девушка в чулках показывает бритую вагину
Пожилая развлекает себя секс игрушкой
Довольную невесту делят между собой два мужчины давая ей понять кайф двойного проникновения

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