Галерея 2823255
Галерея 2823255
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Reser Ranch · Reser Ranch, Updated Rancher - Close to Everything
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Three Bedrooms, 2 Full Bathrooms - Full of Light & Style
Walla Walla County, Washington, United States of America
3. Providence St. Mary Medical Center - Walla Walla 2.3 mi
6. Art Gallery at the Marcus Whitman Hotel & Conference Center 2.4 mi
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You will be responsible for any damage to the rental property caused by you or your party during your stay.
Minimum 1-day vacancy between guest stays
Check in and check out with no person-to-person contact
All towels and bedding washed in hot water that’s at least 60ºC/140ºF
High-touch surfaces cleaned with disinfectant (like countertops, light switches, handles, and faucets)
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Affiliation
1 Department of Medical Microbiology, Stanford University School of Medicine, CA 94305.
R R Spaete et al.
Proc Natl Acad Sci U S A .
1987 Oct .
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1 Department of Medical Microbiology, Stanford University School of Medicine, CA 94305.
Takekoshi M, Maeda-Takekoshi F, Ihara S, Sakuma S, Watanabe Y.
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PMID: 2849236
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PMID: 2993644
Free PMC article.
Chaudhry MZ, Messerle M, Čičin-Šain L.
Chaudhry MZ, et al.
Methods Mol Biol. 2021;2244:133-158. doi: 10.1007/978-1-0716-1111-1_8.
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PMID: 33555586
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Nabavizadeh N, Bressin A, Shboul M, Moreno Traspas R, Chia PH, Bonnard C, Szenker-Ravi E, Sarıbaş B, Beillard E, Altunoglu U, Hojati Z, Drutman S, Freier S, El-Khateeb M, Fathallah R, Casanova JL, Soror W, Arafat A, Escande-Beillard N, Mayer A, Reversade B.
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EMBO Mol Med. 2023 Feb 8;15(2):e16478. doi: 10.15252/emmm.202216478. Epub 2023 Jan 18.
EMBO Mol Med. 2023.
PMID: 36652330
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Tang J, Brixel R, Brune W.
Tang J, et al.
Int J Mol Sci. 2019 Feb 20;20(4):913. doi: 10.3390/ijms20040913.
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PMID: 30791544
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Yamada KH, Majima R, Yamaguchi T, Inoue N.
Yamada KH, et al.
Antivir Chem Chemother. 2018 Jan-Dec;26:2040206618763193. doi: 10.1177/2040206618763193.
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Studies on human cytomegalovirus (CMV) have been limited by a paucity of molecular genetic techniques available for manipulating the viral genome. We have developed methods for site-specific insertion and deletion mutagenesis of CMV utilizing a modified Escherichia coli lacZ gene as a genetic marker. The lacZ gene was placed under the control of the major beta gene regulatory signals and inserted into the viral genome by homologous recombination, disrupting one of two copies of this beta gene within the L-component repeats of CMV DNA. We observed high-level expression of beta-galactosidase by the recombinant in a temporally authentic manner, with levels of this enzyme approaching 1% of total protein in infected cells. Thus, CMV is an efficient vector for high-level expression of foreign gene products in human cells. Using back selection of lacZ-deficient virus in the presence of the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactoside, we generated random endpoint deletion mutants. Analysis of these mutants revealed that CMV DNA sequences flanking the insert had been removed, thereby establishing this approach as a means of determining whether sequences flanking a lacZ insertion are dispensable for viral growth. In an initial test of the methods, we have shown that 7800 base pairs of one copy of L-component repeat sequences can be deleted without affecting viral growth in human fibroblasts.
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