Галерея 2781515

Галерея 2781515

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J Biol Chem

v.284(45); 2009 Nov 6

PMC2781515

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J Biol Chem. 2009 Nov 6; 284(45): 31164–31173.
Published online 2009 Sep 17. doi: 10.1074/jbc.M109.054676
the § International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
1 To whom correspondence should be addressed. Tel.: 81-29-860-4496; Fax: 81-29-860-4714; E-mail: pj.og.smin@nehC.gnipouG .
Copyright © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.
GUID: 6BDB22D2-DB3A-49A8-B278-1B03D97A6016
GUID: 9DCA3E5C-4E44-437C-AF6A-81CDC64BA5DB
ECM extracellular matrix MSC mesenchymal stem cell DMEM Dulbecco's modified Eagle's medium FBS fetal bovine serum TCPS tissue culture polystyrene PBS phosphate-buffered saline BSA bovine serum albumin CBB Coomassie Brilliant Blue.
1. D'Amour K. A., Bang A. G., Eliazer S., Kelly O. G., Agulnick A. D., Smart N. G., Moorman M. A., Kroon E., Carpenter M. K., Baetge E. E. (2006) Nat. Biotechnol. 24 , 1392–1401 [ PubMed ] [ Google Scholar ]
2. Daley W. P., Peters S. B., Larsen M. (2008) J. Cell Sci. 121 , 255–264 [ PubMed ] [ Google Scholar ]
3. Page-McCaw A., Ewald A. J., Werb Z. (2007) Nat. Rev. Mol. Cell. Biol. 8 , 221–233 [ PMC free article ] [ PubMed ] [ Google Scholar ]
4. Pittenger M. F., Mackay A. M., Beck S. C., Jaiswal R. K., Douglas R., Mosca J. D., Moorman M. A., Simonetti D. W., Craig S., Marshak D. R. (1999) Science 284 , 143–147 [ PubMed ] [ Google Scholar ]
5. Malaval L., Modrowski D., Gupta A. K., Aubin J. E. (1994) J. Cell. Physiol. 158 , 555–572 [ PubMed ] [ Google Scholar ]
6. Ducy P., Zhang R., Geoffroy V., Ridall A. L., Karsenty G. (1997) Cell 89 , 747–754 [ PubMed ] [ Google Scholar ]
7. Nakashima K., Zhou X., Kunkel G., Zhang Z., Deng J. M., Behringer R. R., de Crombrugghe B. (2002) Cell 108 , 17–29 [ PubMed ] [ Google Scholar ]
8. Nishio Y., Dong Y., Paris M., O'Keefe R. J., Schwarz E. M., Drissi H. (2006) Gene 372 , 62–70 [ PubMed ] [ Google Scholar ]
9. Nakamura M., Sone S., Takahashi I., Mizoguchi I., Echigo S., Sasano Y. (2005) J. Histochem. Cytochem. 53 , 1553–1562 [ PMC free article ] [ PubMed ] [ Google Scholar ]
10. Sasano Y., Li H. C., Zhu J. X, Imanaka-Yoshida K., Mizoguchi I., Kagayama M. (2000) Histochem. J. 32 , 591–598 [ PubMed ] [ Google Scholar ]
11. Kamiya N., Shigemasa K., Takagi M. (2001) J. Oral Sci. 43 , 179–188 [ PubMed ] [ Google Scholar ]
12. Pham Q. P., Kasper F. K., Scott Baggett L., Raphael R. M., Jansen J. A., Mikos A. G. (2008) Biomaterials 29 , 2729–2739 [ PubMed ] [ Google Scholar ]
13. Xu T., Bianco P., Fisher L. W., Longenecker G., Smith E., Goldstein S., Bonadio J., Boskey A., Heegaard A. M., Sommer B., Satomura K., Dominguez P., Zhao C., Kulkarni A. B., Robey P. G., Young M. F. (1998) Nat. Genet. 20 , 78–82 [ PubMed ] [ Google Scholar ]
14. Chen X. D., Shi S., Xu T., Robey P. G., Young M. F. (2002) J. Bone Miner. Res. 17 , 331–340 [ PubMed ] [ Google Scholar ]
15. Gutierrez J., Osses N., Brandan E. (2006) J. Cell. Physiol. 206 , 58–67 [ PubMed ] [ Google Scholar ]
16. Riquelme C., Larrain J., Schonherr E., Henriquez J. P., Kresse H., Brandan E. (2001) J. Biol. Chem. 276 , 3589–3596 [ PubMed ] [ Google Scholar ]
17. Moursi A. M., Damsky C. H., Lull J., Zimmerman D., Doty S. B., Aota S., Globus R. K. (1996) J. Cell Sci. 109 , 1369–1380 [ PubMed ] [ Google Scholar ]
18. Adachi E., Hopkinson I., Hayashi T. (1997) Int. Rev. Cytol. 173 , 73–156 [ PubMed ] [ Google Scholar ]
19. Gilbert T. W., Sellaro T. L., Badylak S. F. (2006) Biomaterials 27 , 3675–3683 [ PubMed ] [ Google Scholar ]
20. Ott H. C., Matthiesen T. S., Goh S. K., Black L. D., Kren S. M., Netoff T. I., Taylor D. A. (2008) Nat. Med. 14 , 213–221 [ PubMed ] [ Google Scholar ]
21. Datta N., Holtorf H. L., Sikavitsas V. I., Jansen J. A., Mikos A. G. (2005) Biomaterials 26 , 971–977 [ PubMed ] [ Google Scholar ]
22. Datta N., Pham Q. P., Sharma U., Sikavitsas V. I., Jansen J. A., Mikos A. G. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 , 2488–2493 [ PMC free article ] [ PubMed ] [ Google Scholar ]
23. Chen X. D., Dusevich V., Feng J. Q., Manolagas S. C., Jilka R. L. (2007) J. Bone Miner. Res. 22 , 1943–1956 [ PubMed ] [ Google Scholar ]
24. Lehr H. A., Mankoff D. A., Corwin D., Santeusanio G., Gown A. M. (1997) J. Histochem. Cytochem. 45 , 1559–1565 [ PubMed ] [ Google Scholar ]
25. Martin I., Jakob M., Schäfer D., Dick W., Spagnoli G., Heberer M. (2001) Osteoarthritis Cartilage 9 , 112–118 [ PubMed ] [ Google Scholar ]
26. Schaefer J. F., Millham M. L., de Crombrugghe B., Buckbinder L. (2003) Osteoarthritis Cartilage 11 , 233–241 [ PubMed ] [ Google Scholar ]
27. Kanzler B., Kuschert S. J., Liu Y. H., Mallo M. (1998) Development 125 , 2587–2597 [ PubMed ] [ Google Scholar ]
28. Bi W., Deng J. M., Zhang Z, Behringer R. R., de Crombrugghe B. (1999) Nat. Genet. 22 , 85–89 [ PubMed ] [ Google Scholar ]
29. Spiegelman B. M., Flier J. S. (1996) Cell 87 , 377–389 [ PubMed ] [ Google Scholar ]
30. Lee K. S., Kim H. J., Li Q. L., Chi X. Z., Ueta C., Komori T., Wozney J. M., Kim E. G., Choi J. Y., Ryoo H. M., Bae S. C. (2000) Mol. Cell. Biol. 20 , 8783–8792 [ PMC free article ] [ PubMed ] [ Google Scholar ]
31. Lian J. B., Stein G. S., Javed A., van Wijnen A. J., Stein J. L., Montecino M., Hassan M. Q., Gaur T., Lengner C. J., Young D. W. (2006) Rev. Endocr. Metab. Disord. 7 , 1–16 [ PubMed ] [ Google Scholar ]
32. Suzawa M., Takeuchi Y., Fukumoto S., Kato S., Ueno N., Miyazono K., Matsumoto T., Fujita T. (1999) Endocrinology 140 , 2125–2133 [ PubMed ] [ Google Scholar ]
33. Abe E., Yamamoto M., Taguchi Y., Lecka-Czernik B., O'Brien C. A., Economides A. N., Stahl N., Jilka R. L., Manolagas S. C. (2000) J. Bone Miner. Res. 15 , 663–673 [ PubMed ] [ Google Scholar ]
34. Okamura M., Kudo H., Wakabayashi K., Tanaka T., Nonaka A., Uchida A., Tsutsumi S., Sakakibara I., Naito M., Osborne T. F., Hamakubo T., Ito S., Aburatani H., Yanagisawa M., Kodama T., Sakai J. (2009) Proc. Natl. Acad. Sci. U.S.A. 106 , 5819–5824 [ PMC free article ] [ PubMed ] [ Google Scholar ]
35. Park C. H., Chang J. Y., Hahm E. R., Park S., Kim H. K., Yang C. H. (2005) Biochem. Biophys. Res. Commun. 328 , 227–234 [ PubMed ] [ Google Scholar ]
36. Gendron C., Kashiwagi M., Lim N. H., Enghild J. J., Thøgersen I. B., Hughes C., Caterson B., Nagase H. (2007) J. Biol. Chem. 282 , 18294–18306 [ PubMed ] [ Google Scholar ]
37. Dallas S. L., Rosser J. L., Mundy G. R., Bonewald L. F. (2002) J. Biol. Chem. 277 , 21352–21360 [ PubMed ] [ Google Scholar ]
38. Santra M., Reed C. C., Iozzo R. V. (2002) J. Biol. Chem. 277 , 35671–35681 [ PubMed ] [ Google Scholar ]
39. Jeon M. J., Kim J. A., Kwon S. H., Kim S. W., Park K. S., Park S. W., Kim S. Y., Shin C. S. (2003) J. Biol. Chem. 278 , 23270–23277 [ PubMed ] [ Google Scholar ]
40. Hocking A. M., Shinomura T., McQuillan D. J. (1998) Matrix Biol. 17 , 1–19 [ PubMed ] [ Google Scholar ]
41. Moreno M., Muñoz R., Aroca F., Labarca M., Brandan E., Larraín J. (2005) EMBO J. 24 , 1397–1405 [ PMC free article ] [ PubMed ] [ Google Scholar ]
42. Kang S., Bennett C. N., Gerin I., Rapp L. A., Hankenson K. D., Macdougald O. A. (2007) J. Biol. Chem. 282 , 14515–14524 [ PubMed ] [ Google Scholar ]
43. Liu G., Vijayakumar S., Grumolato L., Arroyave R., Qiao H., Akiri G., Aaronson S. A. (2009) J. Cell Biol. 185 , 67–75 [ PMC free article ] [ PubMed ] [ Google Scholar ]
44. Reichsman F., Smith L., Cumberledge S. (1996) J. Cell Biol. 135 , 819–827 [ PMC free article ] [ PubMed ] [ Google Scholar ]
45. Takeuchi T., Ochiya T., Takezawa T. (2008) Tissue Eng. Part A 14 , 267–274 [ PubMed ] [ Google Scholar ]
Articles from The Journal of Biological Chemistry are provided here courtesy of American Society for Biochemistry and Molecular Biology
1. D'Amour K. A., Bang A. G., Eliazer S., Kelly O. G., Agulnick A. D., Smart N. G., Moorman M. A., Kroon E., Carpenter M. K., Baetge E. E. (2006) Nat. Biotechnol. 24 , 1392–1401 [ PubMed ] [ Google Scholar ] [ Ref list ]
2. Daley W. P., Peters S. B., Larsen M. (2008) J. Cell Sci. 121 , 255–264 [ PubMed ] [ Google Scholar ] [ Ref list ]
3. Page-McCaw A., Ewald A. J., Werb Z. (2007) Nat. Rev. Mol. Cell. Biol. 8 , 221–233 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
4. Pittenger M. F., Mackay A. M., Beck S. C., Jaiswal R. K., Douglas R., Mosca J. D., Moorman M. A., Simonetti D. W., Craig S., Marshak D. R. (1999) Science 284 , 143–147 [ PubMed ] [ Google Scholar ] [ Ref list ]
5. Malaval L., Modrowski D., Gupta A. K., Aubin J. E. (1994) J. Cell. Physiol. 158 , 555–572 [ PubMed ] [ Google Scholar ] [ Ref list ]
6. Ducy P., Zhang R., Geoffroy V., Ridall A. L., Karsenty G. (1997) Cell 89 , 747–754 [ PubMed ] [ Google Scholar ] [ Ref list ]
7. Nakashima K., Zhou X., Kunkel G., Zhang Z., Deng J. M., Behringer R. R., de Crombrugghe B. (2002) Cell 108 , 17–29 [ PubMed ] [ Google Scholar ] [ Ref list ]
8. Nishio Y., Dong Y., Paris M., O'Keefe R. J., Schwarz E. M., Drissi H. (2006) Gene 372 , 62–70 [ PubMed ] [ Google Scholar ] [ Ref list ]
9. Nakamura M., Sone S., Takahashi I., Mizoguchi I., Echigo S., Sasano Y. (2005) J. Histochem. Cytochem. 53 , 1553–1562 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
10. Sasano Y., Li H. C., Zhu J. X, Imanaka-Yoshida K., Mizoguchi I., Kagayama M. (2000) Histochem. J. 32 , 591–598 [ PubMed ] [ Google Scholar ] [ Ref list ]
11. Kamiya N., Shigemasa K., Takagi M. (2001) J. Oral Sci. 43 , 179–188 [ PubMed ] [ Google Scholar ] [ Ref list ]
12. Pham Q. P., Kasper F. K., Scott Baggett L., Raphael R. M., Jansen J. A., Mikos A. G. (2008) Biomaterials 29 , 2729–2739 [ PubMed ] [ Google Scholar ] [ Ref list ]
13. Xu T., Bianco P., Fisher L. W., Longenecker G., Smith E., Goldstein S., Bonadio J., Boskey A., Heegaard A. M., Sommer B., Satomura K., Dominguez P., Zhao C., Kulkarni A. B., Robey P. G., Young M. F. (1998) Nat. Genet. 20 , 78–82 [ PubMed ] [ Google Scholar ] [ Ref list ]
14. Chen X. D., Shi S., Xu T., Robey P. G., Young M. F. (2002) J. Bone Miner. Res. 17 , 331–340 [ PubMed ] [ Google Scholar ] [ Ref list ]
17. Moursi A. M., Damsky C. H., Lull J., Zimmerman D., Doty S. B., Aota S., Globus R. K. (1996) J. Cell Sci. 109 , 1369–1380 [ PubMed ] [ Google Scholar ] [ Ref list ]
18. Adachi E., Hopkinson I., Hayashi T. (1997) Int. Rev. Cytol. 173 , 73–156 [ PubMed ] [ Google Scholar ] [ Ref list ]
19. Gilbert T. W., Sellaro T. L., Badylak S. F. (2006) Biomaterials 27 , 3675–3683 [ PubMed ] [ Google Scholar ] [ Ref list ]
20. Ott H. C., Matthiesen T. S., Goh S. K., Black L. D., Kren S. M., Netoff T. I., Taylor D. A. (2008) Nat. Med. 14 , 213–221 [ PubMed ] [ Google Scholar ] [ Ref list ]
21. Datta N., Holtorf H. L., Sikavitsas V. I., Jansen J. A., Mikos A. G. (2005) Biomaterials 26 , 971–977 [ PubMed ] [ Google Scholar ] [ Ref list ]
22. Datta N., Pham Q. P., Sharma U., Sikavitsas V. I., Jansen J. A., Mikos A. G. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 , 2488–2493 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
23. Chen X. D., Dusevich V., Feng J. Q., Manolagas S. C., Jilka R. L. (2007) J. Bone Miner. Res. 22 , 1943–1956 [ PubMed ] [ Google Scholar ] [ Ref list ]
24. Lehr H. A., Mankoff D. A., Corwin D., Santeusanio G., Gown A. M. (1997) J. Histochem. Cytochem. 45 , 1559–1565 [ PubMed ] [ Google Scholar ] [ Ref list ]
25. Martin I., Jakob M., Schäfer D., Dick W., Spagnoli G., Heberer M. (2001) Osteoarthritis Cartilage 9 , 112–118 [ PubMed ] [ Google Scholar ] [ Ref list ]
26. Schaefer J. F., Millham M. L., de Crombrugghe B., Buckbinder L. (2003) Osteoarthritis Cartilage 11 , 233–241 [ PubMed ] [ Google Scholar ] [ Ref list ]
27. Kanzler B., Kuschert S. J., Liu Y. H., Mallo M. (1998) Development 125 , 2587–2597 [ PubMed ] [ Google Scholar ] [ Ref list ]
28. Bi W., Deng J. M., Zhang Z, Behringer R. R., de Crombrugghe B. (1999) Nat. Genet. 22 , 85–89 [ PubMed ] [ Google Scholar ] [ Ref list ]
29. Spiegelman B. M., Flier J. S. (1996) Cell 87 , 377–389 [ PubMed ] [ Google Scholar ] [ Ref list ]
30. Lee K. S., Kim H. J., Li Q. L., Chi X. Z., Ueta C., Komori T., Wozney J. M., Kim E. G., Choi J. Y., Ryoo H. M., Bae S. C. (2000) Mol. Cell. Biol. 20 , 8783–8792 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
31. Lian J. B., Stein G. S., Javed A., van Wijnen A. J., Stein J. L., Montecino M., Hassan M. Q., Gaur T., Lengner C. J., Young D. W. (2006) Rev. Endocr. Metab. Disord. 7 , 1–16 [ PubMed ] [ Google Scholar ] [ Ref list ]
32. Suzawa M., Takeuchi Y., Fukumoto S., Kato S., Ueno N., Miyazono K., Matsumoto T., Fujita T. (1999) Endocrinology 140 , 2125–2133 [ PubMed ] [ Google Scholar ] [ Ref list ]
33. Abe E., Yamamoto M., Taguchi Y., Lecka-Czernik B., O'Brien C. A., Economides A. N., Stahl N., Jilka R. L., Manolagas S. C. (2000) J. Bone Miner. Res. 15 , 663–673 [ PubMed ] [ Google Scholar ] [ Ref list ]
34. Okamura M., Kudo H., Wakabayashi K., Tanaka T., Nonaka A., Uchida A., Tsutsumi S., Sakakibara I., Naito M., Osborne T. F., Hamakubo T., Ito S., Aburatani H., Yanagisawa M., Kodama T., Sakai J. (2009) Proc. Natl. Acad. Sci. U.S.A. 106 , 5819–5824 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
35. Park C. H., Chang J. Y., Hahm E. R., Park S., Kim H. K., Yang C. H. (2005) Biochem. Biophys. Res. Commun. 328 , 227–234 [ PubMed ] [ Google Scholar ] [ Ref list ]
36. Gendron C., Kashiwagi M., Lim N. H., Enghild J. J., Thøgersen I. B., Hughes C., Caterson B., Nagase H. (2007) J. Biol. Chem. 282 , 18294–18306 [ PubMed ] [ Google Scholar ] [ Ref list ]
37. Dallas S. L., Rosser J. L., Mundy G. R., Bonewald L. F. (2002) J. Biol. Chem. 277 , 21352–21360 [ PubMed ] [ Google Scholar ] [ Ref list ]
38. Santra M., Reed C. C., Iozzo R. V. (2002) J. Biol. Chem. 277 , 35671–35681 [ PubMed ] [ Google Scholar ] [ Ref list ]
39. Jeon M. J., Kim J. A., Kwon S. H., Kim S. W., Park K. S., Park S. W., Kim S. Y., Shin C. S. (2003) J. Biol. Chem. 278 , 23270–23277 [ PubMed ] [ Google Scholar ] [ Ref list ]
15. Gutierrez J., Osses N., Brandan E. (2006) J. Cell. Physiol. 206 , 58–67 [ PubMed ] [ Google Scholar ] [ Ref list ]
16. Riquelme C., Larrain J., Schonherr E., Henriquez J. P., Kresse H., Brandan E. (2001) J. Biol. Chem. 276 , 3589–3596 [ PubMed ] [ Google Scholar ] [ Ref list ]
40. Hocking A. M., Shinomura T., McQuillan D. J. (1998) Matrix Biol. 17 , 1–19 [ PubMed ] [ Google Scholar ] [ Ref list ]
41. Moreno M., Muñoz R., Aroca F., Labarca M., Brandan E., Larraín J. (2005) EMBO J. 24 , 1397–1405 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
42. Kang S., Bennett C. N., Gerin I., Rapp L. A., Hankenson K. D., Macdougald O. A. (2007) J. Biol. Chem. 282 , 14515–14524 [ PubMed ] [ Google Scholar ] [ Ref list ]
43. Liu G., Vijayakumar S., Grumolato L., Arroyave R., Qiao H., Akiri G., Aaronson S. A. (2009) J. Cell Biol. 185 , 67–75 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
44. Reichsman F., Smith L., Cumberledge S. (1996) J. Cell Biol. 135 , 819–827 [ PMC free article ] [ PubMed ] [ Google Scholar ] [ Ref list ]
45. Takeuchi T., Ochiya T., Takezawa T. (2008) Tissue Eng. Part A 14 , 267–274 [ PubMed ] [ Google Scholar ] [ Ref list ]

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the § International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
the § International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
An extracellular microenvironment, including an extracellular matrix (ECM), is an important factor in regulating stem cell differentiation. During tissue development, the ECM is dynamically remodeled to regulate stem cell functions. Here, we developed matrices mimicking ECM remodeling during the osteogenesis of mesenchymal stem cells (MSCs). The matrices were prepared from cultured MSCs controlled at different stages of osteogenesis and referred to as “stepwise osteogenesis-mimicking matrices.” The matrices supported the adhesion and proliferation of MSCs and showed different effects on the osteogenesis of MSCs. On the matrices mimicking the early stage of osteogenesis (early stage matrices), the osteogenesis occurred more rapidly than did that on the matrices mimicking undifferentiated stem cells (stem cell matrices) and the late stage of osteogenesis (late stage matrices). RUNX2 was similarly expressed when MSCs were cultured on both the early stage and late stage matrices but decreased on the stem cell matrices. PPARG expression in the MSCs cultured on the late stage matrices was higher than for those cultured on the stem cell and early stage matrices. This increase of PPARG expression was caused by the suppression of the amount of β-catenin and downstream signal transduction. These results demonstrate that the osteogenesis-mimicking matrices had different effects on the osteogenesis of MSCs, and the early stage matrices provided a favorable microenvironment for the osteogenesis.
Stem cells pass through stepwise maturational stages for differentiation into somatic cells. During the stepwise differentiation of stem cells into somatic cells, the expression pattern of transcription factors changes, depending on their maturational stages ( 1 ). The expression of the
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