Fulllength smolina part
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Ekaterina Smolina Photos and Premium High Res Pictures . . .
Toehold-enhanced LNA probes for selective pull down and . . .
End invasion of peptide nucleic acids (PNAs) with mixed . . .
Toehold-enhanced LNA probes for selective pull down and . . .
(PDF) End invasion of peptide nucleic acids (PNAs) with . . .
Single-cell identification in microbial communities by . . .
Knockout of zebrafish desmin genes does not cause skeletal . . .
Cells | Free Full-Text | Functions of Vertebrate Ferlins . . .
Comparing the contribution of prescribed opioids to opioid . . .
Estimates of opioid use disorder prevalence from a . . .
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In a similar fashion, Smolina et al . showed the inability of PNA oligonucleotides to invade internal sites of a double helix, whereas end-duplexes were readily formed 14 .
INTRODUCTION . Recognition of specific sequences within double-stranded (ds) DNA via ligand binding is a general and potent strategy for probing DNA functions, and for the control of gene expression (1, 2) .Synthetic oligonucleotide analogs and mimics, which can bind to DNA more strongly than regular oligonucleotides, and without the loss of specificity, are of major interest in this respect () .
In a similar fashion, Smolina et al . showed the inability of PNA oligonucleotides to invade internal sites of a double helix, whereas end-duplexes were readily formed 14 . LNA probes were also shown before to invade DNA ends more readily 20 , but the addition of a small toehold as we show here, speeds up the reaction significantly and allows for . . .
The full-length product of the primer-extension we observed were formed via strand invasion, we investigated reaction is expected to be 137 or 508 bp long for AatII/ the affinity capture of a restriction fragment carrying the target PvuII and AatII restriction fragment, respectively, which con- site in the presence of the rest of plasmid DNA as . . .
Amann and Fuchs provide an update on recent methodological improvements to fluorescencein situhybridization protocols, with a particular focus on whether the original group-specific probes, which . . .
Desmin is a muscle-specific intermediate filament protein that has fundamental role in muscle structure and force transmission . Whereas human desmin protein is encoded by a single gene, two desmin paralogs (desma and desmb) exist in zebrafish .Desma and desmb show differential spatiotemporal expression during zebrafish embryonic and larval development, being similarly expressed in skeletal . . .
Unfortunately, 3D structures of full-length ferlins are still unknown, as it is difficult to isolate the full-length proteins . To date, structures of the C2A domains of otoferlin, dysferlin and myoferlin [42,52,53], of FerA domains of the same proteins , and of the inner DysF domains of human dysferlin and myoferlin have been resolved [54,55 . . .
Overall, the prevalence of active opioid prescribing prior to an opioid-related hospitalization was high in all provinces studied but was much lower in BC (34 .1%) compared to both Manitoba (52 .2%) and Ontario (52 .8%) (p < 0 .05; Table 2) .Among those with an active opioid prescription, the median time between the last opioid dispensing and overdose was 3 days in both BC and Manitoba . . .
1 . Introduction . An epidemic of opioid use disorder (OUD) and opioid overdose has spread across North America, with overdose emerging as a leading cause of death in recent years (Gostin et al ., 2017; Rudd et al ., 2016; Ye et al ., 2018) .With fentanyl as a catalyst, the Canadian province of British Columbia (BC) has been disproportionately affected and has a rate of overdose death nearly three . . .
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Fulllength smolina part
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