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Affiliation
1 Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
Bixing Huang et al.
J Bacteriol .
2003 Dec .
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1 Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
Schematic representation of the fimX locus. The relevant restriction sites are indicated. (A) Overall topography of the fimX region. The open arrows indicate the relative transcriptional orientations of fimX and its neighboring genes, and Tt indicates the predicted transcription terminator following fimX . (B) Expanded view of fimX . The rectangles indicate the predicted domains in FimX. The transposon insertion sites in fimX mutants S19, S46, and S58 are indicated by solid triangles. The orientations of fimX in derived plasmid constructs are indicated by arrows; the arrows on the left indicate that the fimX coding region is in the same orientation as the adjacent lac promoter, and the arrows on the right indicate that the fimX coding region is in the same orientation as the adjacent T7 promoter. Note that the Not I and last Sal I restriction sites were derived from the multiple cloning site of the pUCPKS and pUCPSK vectors, which were used to construct translational fusions with RFP for subcellular localization studies.
Macroscopic and microscopic examination of twitching motility in fimX mutants. (A to F) Twitching zones observed in the subsurface stab assay on agar plates after 24 h of growth. Bar = 1 cm. (G to L) Light microscopy of the edges of the twitching zones at the interstitial surfaces between the glass coverslips and GelGro medium. Bar = 10 μm. (A and G) Wild-type strain PAK; (B and H) PAKΔ pilA mutant; (C and I) fimX mutant S19; (D and J) S19(pUCPSK); (E and K) S19(pBH51); (F and L) S19(pBH52). The medium used for the subsurface twitching assay in complementation studies with the control vector or vectors containing fimX sequences contained 250 μg of carbenicillin ml −1 . Similar results were obtained for complementation of mutants S46 and S58 (data not shown).
Western and ELISA analyses of pilus production in fimX mutants. (A) Surface pili extracted from PAK (lane 1), PAKΔ pilA (lane 2), PAKΔ pilV (lane 3), PAK(pUCPSK) (lane 4), PAK(pBH52) (lane 5), S19 (lane 6), S19(pUCPSK) (lane 7), and S19(pBH52) (lane 8). The gel was stained with Coomassie brilliant blue R250. (B) Western blotting of the surface pili from the same strains that were used in panel A. (C) Western blotting of the whole-cell proteins from the same strains that were used in panel A. (D) Quantitative analysis of the level of surface pili by ELISA for PAK (♦), PAKΔ pilA (solid line), S19 (▴), S19(pBH51) (•), and S19(pBH52) (*). OD, optical density.
Comparison of the CheY-like, DUF1, and DUF2 domains of FimX with the domains of other proteins. (A) Alignment of the CheY-like domain of FimX with domains of other typical CheY-containing proteins. The five conserved functional sites identified by Volz (51) are indicated by asterisks. The sources of the CheY sequences (with their accession numbers) are as follows: CheY_Mdeg2095, putative CheY-like domain of Mdeg2095 in M. degradans ; CheY_XAC2398, putative CheY-like domain of XAC2398 in X. axonopodis ; CheY_Pa, CheY of P. aeruginosa (AAG04845); CheY_Ec, CheY of E. coli (NP_416396). (B and C) Alignment of the DUF1 and DUF2 domains of FimX with the corresponding domains of PleD, PdeA1 to PdeA3 and Dgc1 to Dgc3. The conserved functional sites in DUF1 and DUF2 are indicated by asterisks. The sequences are from references and .
Localization of RFP fused with full-length FimX and with the CheY-like domain of FimX in PAK and S19. (A) PAK(pBH223) (RFP fused to FimX); (B) S19(pBH223); (C) PAK(pBH250) (RFP fused to the N-terminal region of FimX containing the CheY-like domain and adjacent sequences); (D) S19(pBH250); (E) PAK(pBH210) (RFP control); (F) S19(pBH210). Panels A to D were photographed with a dual light source to reveal the location of fluorescence in relation to the cell as a whole. The background is reduced in panels E and F, as only red fluorescent light and no background bright-field light were used in these cases.
Alignment of amino acid sequence adjacent to the CheY-like domain of FimX with amino acid sequences implicated in polar localization in other bacteria. The amino acid sequence between the Sal I site and the end of the CheY-like domain of FimX (SalI_FimX) was compared to the equivalent regions of other proteins implicated in polar localization in P. aeruginosa and other species, including PilS in P. aeruginosa (PilS_Pa) (20), MinD in N. gonorrhoeae (MinD_Ng) (36), CheZ in E. coli (CheZ_Ec) (10), and DivJ in C. crescentus (DivJ_Cc) (40).
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Twitching motility is a form of surface translocation mediated by the extension, tethering, and retraction of type IV pili. Three independent Tn5-B21 mutations of Pseudomonas aeruginosa with reduced twitching motility were identified in a new locus which encodes a predicted protein of unknown function annotated PA4959 in the P. aeruginosa genome sequence. Complementation of these mutants with the wild-type PA4959 gene, which we designated fimX, restored normal twitching motility. fimX mutants were found to express normal levels of pilin and remained sensitive to pilus-specific bacteriophages, but they exhibited very low levels of surface pili, suggesting that normal pilus function was impaired. The fimX gene product has a molecular weight of 76,000 and contains four predicted domains that are commonly found in signal transduction proteins: a putative response regulator (CheY-like) domain, a PAS-PAC domain (commonly involved in environmental sensing), and DUF1 (or GGDEF) and DUF2 (or EAL) domains, which are thought to be involved in cyclic di-GMP metabolism. Red fluorescent protein fusion experiments showed that FimX is located at one pole of the cell via sequences adjacent to its CheY-like domain. Twitching motility in fimX mutants was found to respond relatively normally to a range of environmental factors but could not be stimulated by tryptone and mucin. These data suggest that fimX is involved in the regulation of twitching motility in response to environmental cues.
Schematic representation of the fimX…
Schematic representation of the fimX locus. The relevant restriction sites are indicated. (A)…
Macroscopic and microscopic examination of…
Macroscopic and microscopic examination of twitching motility in fimX mutants. (A to F)…
Western and ELISA analyses of pilus production in fimX mutants. (A) Surface pili…
Comparison of the CheY-like, DUF1,…
Comparison of the CheY-like, DUF1, and DUF2 domains of FimX with the domains…
Localization of RFP fused with full-length FimX and with the CheY-like domain of…
Alignment of amino acid sequence adjacent to the CheY-like domain of FimX with…
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