FRANCESCA TORRENT
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A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine). This is then reported as a text string, called a read. Some DNA sequencers can be also considered optical instruments as they analyze light signals originating from fluorochromes attached to nucleotides. The first automated DNA sequencer, invented by Lloyd M. Smith, was introduced by Applied Biosystems in 1987. It used the Sanger sequencing method, a technology which formed the basis of the "first generation" of DNA sequencers and enabled the completion of the human genome project in 2001. This first generation of DNA sequencers are essentially automated electrophoresis systems that detect the migration of labelled DNA fragments. Therefore, these sequencers can also be used in the genotyping of genetic markers where only the length of a DNA fragment(s) needs to be determined (e.g. microsatellites, AFLPs). The Human Genome Project spurred the development of cheaper, high throughput and more accurate platforms known as Next Generation Sequencers (NGS) to sequence the human genome. These include the 454, SOLiD and Illumina DNA sequencing platforms. Next generation sequencing machines have increased the rate of DNA sequencing substantially, as compared with the previous Sanger methods. DNA samples can be prepared automatically in as little as 90 mins, while a human genome can be sequenced at 15 times coverage in a matter of days. More recent, third-generation DNA sequencers such as PacBio SMRT and Oxford Nanopore offer the possibility of sequencing long molecules, compared to short-read technologies such as Illumina SBS or MGI Tech's DNBSEQ. Because of limitations in DNA sequencer technology, the reads of many of these technologies are short, compared to the length of a genome therefore the reads must be assembled into longer contigs. The data may also contain errors, caused by limitations in the DNA sequencing technique or by errors during PCR amplification. DNA sequencer manufacturers use a number of different methods to detect which DNA bases are present. The specific protocols applied in different sequencing platforms have an impact in the final data that is generated. Therefore, comparing data quality and cost across different technologies can be a daunting task. Each manufacturer provides their own ways to inform sequencing errors and scores. However, errors and scores between different platforms cannot always be compared directly. Since these systems rely on different DNA sequencing approaches, choosing the best DNA sequencer and method will typically depend on the experiment objectives and available budget.
In connection with: DNA sequencer
Title combos: sequencer DNA
Description combos: light systems compared SOLiD signals manufacturers to can cost
Torrent or torrents may refer to: A fast flowing stream
In connection with: Torrent
Description combos: to fast stream to Torrent Torrent or may fast
The Tulse Luper Suitcases is a multimedia project by film maker and artist Peter Greenaway, initially intended to comprise four films, a 16-episode TV series, and 92 DVDs, as well as websites, CD-ROMs and books. The project documented the imagined life of a fictional character called Tulse Luper. In the online component of the project, web designers competed to make a game based on the interactive site, The Tulse Luper Journey. The winner took a trip following Tulse Luper's travels: subsequent to this, a final feature film was released.
In connection with: The Tulse Luper Suitcases
Title combos: Luper The The Suitcases Tulse Luper Tulse The Suitcases
Description combos: series and Greenaway competed the by Luper of of
Ana Torrent Bertrán de Lis (born 12 July 1966) is a Spanish film actress. She featured as a child actress in pictures such as The Spirit of the Beehive (1973), and Cría cuervos (1976), Elisa, vida mía (1977) and The Nest (1980). Her performance in Thesis (1996) earned her a nomination to the Goya Award for Best Actress.
In connection with: Ana Torrent
Title combos: Torrent Ana
Description combos: the vida the 1976 born Bertrán featured 1996 12
Torrents of Spring (1989 film)
Torrents of Spring is a 1989 drama film written and directed by Jerzy Skolimowski and starring Timothy Hutton, Nastassja Kinski and Valeria Golino. It is based on the 1872 novel by Ivan Turgenev. It is a British, French, and Italian co-production. Set in 1840, the film follows a young Russian aristocrat, Dimitri Sanin, who is torn between the love of a beautiful German pastry-shop girl, Gemma Rosselli, and a Russian seductress, Princess Maria Nikolaevna. The film competed for the Golden Palm Award at the 1989 Cannes Film Festival.
In connection with: Torrents of Spring (1989 film)
Title combos: 1989 Torrents Torrents of Spring Spring Torrents of 1989
Description combos: by Golden Torrents Sanin Set production aristocrat Torrents film
The Haunting or The Beckoning (Spanish: NO-DO) is a Spanish supernatural mystery horror film written and directed by Elio Quiroga which stars Ana Torrent.
In connection with: The Haunting (2009 film)
Title combos: The film 2009 Haunting The 2009 Haunting film The
Description combos: Beckoning DO or Torrent The The film is Haunting
Francesca Torrent Boschdemont (Agullana, Alt Empordà, April 18, 1881 – Barcelona, April 15, 1958) was a Spanish writer. She sometimes used the pseudonym Miosotis.
In connection with: Francesca Torrent
Title combos: Torrent Francesca
Description combos: 1881 Francesca Torrent 18 1881 Francesca April Torrent Alt
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