Eva Green Nide
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Eva Green Nide
GelRedĀ® & GelGreenĀ®
|
EMBER500ā¢ RNA Stain
DNAzureĀ® Visible DNA Stain
EvaGreenĀ® Dye
|
Cheetahā¢ Taq
Forget-Me-Notā¢ qPCR Master Mixes
EMBER500ā¢ RNA Stain
|
AccuBlueĀ® RNA Quant
|
RNAstormā¢ RNA Extraction
EverBriteā¢ Mounting Medium
Super-Resolution Microscopy
Tyramide Signal Amplification
AccuGreenā¢ DNA for QubitĀ®
|
AccuClearĀ® DNA
AccuBlueĀ® RNA
Cell Quantitation
|
Live & Dead Stains
NucViewĀ® Caspase-3 Substrates
Nuclear Stains
|
MitoViewā¢ Dyes
Membrane/Cell Surface Stains
ExoBriteā¢ EV Membrane Stains
ExoBriteā¢ Antibody Conjugates
Reactive Dyes
|
Bioconjugates
Near-IR CFĀ® Dyes
Protein Labeling Kits
|
Small Ligand Labeling
Mix-n-Stainā¢ Antibody Labeling Kits
Primary Antibodies
|
Secondary Antibodies
Search with Antibody Finder
IF Background Suppressor
|
WB Blocking Buffer
Autofluorescence Quencher
ViaFluorĀ® Cell Proliferation
|
Live-or-Dyeā¢ Viability
|
Dyes for Spectral Flow
Firefly Assay
|
Renilla Assay
|
Steady-Lucā¢
One-Step Protein Gel Stains
|
Western Blotting
GloMeltā¢ Protein Thermal Stability Assay
RNAstormā¢ FFPE
|
DNAstormā¢ FFPE
Bacterial Stains
|
Yeast Stains
PMA & PMAxxā¢ v-PCR Dyes
|
v-PCR Starter Kits
Strain-Specific Kits
EVAGREENĀ® DYE
For qPCR & Other Applications
Brighter and less inhibitory than SYBRĀ® Green
Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain
The only dye that allows you to perform qPCR and high-resolution melt (HRM) analysis in the same reaction
EvaGreenĀ® Dye is compatible with a number of digital PCR platforms
EvaGreenĀ® PCR products can be run on a DNA gel and visualized without the need for another gel stain
EvaGreenĀ® Dye is used in all of Biotium's dye-based qPCR master mixes, like the superior Forget-Me-Notā¢ Master Mixes
Environmentally safe
Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain.
Superior for qPCR and isothermal amplification
Far brighter than SYBRĀ® Green I for detecting amplification due to its novel ārelease-on-demandā DNA-binding mechanism.
Unrivaled DNA melt curve performance
Low PCR inhibition permits the use of saturating dye concentration for maximal signal and high-resolution DNA melt analysis.
Extremely stable
Stable during storage or under PCR conditions.
Serves both as a qPCR dye and a DNA gel stain
After the PCR reaction, directly visualize the PCR product on a gel using a UV transilluminator or blue light box without the need for another gel stain.
Compatible with multiplex PCR
Lack of dye migration from amplicon to amplicon enables detection of multiple PCR products by melt curves.
Useful for other applications
Currently the only qPCR dye to be used in droplet digital PCR (ddPCR). EvaGreenĀ® can also be used for isothermal amplification, microfluidic PCR systems, capillary gel electrophoresis, and more.
Figure 3. A comparison of the raw fluorescence signal from qPCR reactions performed with two EvaGreenĀ® master mixes (Forget-Me-Notā¢ EvaGreenĀ® and Fast EvaGreenĀ®) and QuantiNova SYBRĀ® Green. EvaGreenĀ® Dye is less inhibitory than SYBRĀ® green, allowing for a much brighter signal.
Figure 4. EvaGreenĀ® Dye is safer than SYBRĀ® Green I due to the fact that it is cell membrane impermeable, and therefore cannot gain access to the DNA in the nuclei of living cells.
*Performing HRM may require a license from Idaho Technology.
From: $ 92
Sizes: Trial size (1 mL), 5 x 1 mL Catalog #: 31077-T , - 31077 View all Hide
From: $ 49
Sizes: Trial size (1 mL), 5 x 1 mL Catalog #: 31000 , - 31000-T View all Hide
Forget-Me-Notā¢ EvaGreenĀ® qPCR Master Mix (Low ROX or High ROX)
From: $ 63
Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions) Catalog #: 31045-1mL , 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mL View all Hide
Forget-Me-Notā¢ EvaGreenĀ® qPCR Master Mix (2-Color Tracking)
From: $ 66
Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions) Catalog #: 31041-T , 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mL View all Hide
EvaGreenĀ® Dye is a next-generation DNA-binding dye that is ideal for quantitative real-time PCR (qPCR) and many other applications. Biotium scientists designed the dye by taking into consideration several essential dye properties relevant to PCR, including PCR inhibition, safety, stability and fluorescence spectra of the dye. The result of our efforts is a dye superior to SYBRĀ® Green I and other commercial PCR or high-resolution melt curve (HRM) dyes.*
EvaGreenĀ® Dye is used in all of Biotiumās dye-based qPCR master mixes, which combine superior brightness and sensitivity, with the ability to do sensitive melt curve analysis in the same reaction. In addition, EvaGreenĀ® Dye is non-toxic, non-mutagenic, and not hazardous to aquatic life.
EvaGreenĀ® Plus Dye is an improved alternative to Biotiumās original EvaGreenĀ® Dye which retains the same essential benefits while providing a greater signal-to-noise ratio. The lower background and higher signal from EvaGreenĀ® Plus provides further advantages for digital PCR and isothermal applications.
Visit the Forget-Me-Notā¢ EvaGreenĀ® qPCR Master Mix technology page to learn more about the advantages of our qPCR reagents.
EvaGreenĀ® DNA-binding dye is designed using a novel concept of DNA binding via ārelease-on-demandā mechanism (Fig. 1). The dye is constructed of two monomeric DNA-binding dyes linked by a flexible spacer. In the absence of DNA, the dimeric dye assumes a looped conformation that is inactive in DNA binding. When DNA is available, the looped conformation shifts via an equilibrium to a random conformation that is capable of binding to DNA to emit fluorescence.
This chemical equilibrium provides a unique mechanism to continuously supply the active form of the dye from the āreserveā (i.e., the dye in looped conformation), as more DNA is formed during a PCR process. Consequently, an EvaGreenĀ® master mix can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition, making the mix suitable for both qPCR and HRM applications.*
A PCR dye emits fluorescence by forming a dye-DNA complex. The interaction with DNA inevitably leads to some PCR interference. PCR inhibition by the dye can be particularly serious at the early stage of PCR, where the dye-to-DNA ratio is high. On the other hand, a sufficient dye concentration is important for generating good signal. Thus, an optimal dye concentration must be used in order to attain reliable PCR performance.
For many current DNA-binding PCR dyes, such as SYBRĀ® Green I, the optimal dye concentration can be quite low, which limits PCR signal and also makes the dyes unsuitable for high-resolution melt curve (HRM) analysis. Furthermore, a master mix with low SYBRĀ® Green concentration may fail to detect multiple amplicons by melt peaks due to dye migration from small amplicons to large amplicons, giving the false result of a clean single amplicon for a PCR that may, in fact, produce several products.
See the full list of references here .
Highlighted Citation: The superior performance demonstrated by EvaGreenĀ® dye has allowed it to be the first and only DNA-binding dye suitable for ddPCR applications. Initially designed for fluorescent probe-based detection, ddPCR technology has enabled quantitative multiplex PCR by partitioning samples into thousands of droplets that can be individually measured. In a publication in Analytical Chemistry, McDermott et al. demonstrate how EvaGreenĀ® dye can be used with ddPCR to analyze multiple targets within a sample by distinguishing the amplified DNA mass per droplet. Results show EvaGreenĀ®-based ddPCR performs comparably with TaqManĀ®-based ddPCR, without the added complexity and cost of fluorescent probes. EvaGreenĀ® Dye is currently licensed for Bio-Radās QX200 ddPCRā¢ EvaGreenĀ® Supermix and other ddPCR related reagents.
Another major advantage of EvaGreenĀ® Dye over other PCR and HRM dyes is its safety. EvaGreenĀ® Dye is the first and only PCR dye to date designed to be environmentally safe.
Very few PCR dyes have been thoroughly studied for their safety despite the increasing use of PCR in research and diagnostics and the fact that DNA-binding dyes are inherently dangerous due to their potential to cause mutation.
Thus, handling and disposal of PCR master mixes can be a health and environmental issue. Indeed, SYBRĀ® Green I is found to be even more environmentally toxic than ethidium bromide, one of the best known mutagens. SYBRĀ® Green I has been suggested to interfere with DNA repair mechanisms in cells, and as a result it potentiates genotoxicity of chemicals as well as DNA damage by UV light.
Although no safety data are available on other PCR and HRM dyes (e.g., SYTO9, LC Green, BRYT Green and ResoLight), those dyes are all known to enter cells in a matter of minutes, thus posing potential genotoxicity risk. With this in mind, Biotiumās scientists designed EvaGreenĀ® Dye to be cell membrane impermeable by increasing the molecular size and charge of the dye (Figure 4). Because EvaGreenĀ® Dye is denied the chance to interact with genomic DNA in living cells, it is made much safer than the other dyes. Independent laboratory tests have confirmed that EvaGreenĀ® is non-mutagenic, non-cytotoxic and safe to aquatic life. The dye has passed environmental hazardous waste regulation in the state of California (CCR title 22) for easy disposal down the drain. See the EvaGreenĀ® Safety Report for more information.
EvaGreenĀ® Dye is very stable both during storage and under PCR conditions. EvaGreenĀ® Dye has been reported to be stable at temperatures as high as 65Ā°C for up to 6 months of use in the field for real-time PCR ( Mil Med. 2014 179(6):626-32 ).
SYBRĀ® Green I, on the other hand, is known to degrade following multiple freeze-thaw cycles and under PCR conditions. Moreover, decomposed SYBRĀ® Green I is reported to be even more inhibitory to PCR. Thus, when assessing the performance of an EvaGreenĀ®-based master mix, you can eliminate the stability of the dye as a variable.
EvaGreenĀ® Dye is spectrally similar to FAM or SYBRĀ® Green I, which means no change in optical settings is required for using an EvaGreenĀ®-based master mix.
EvaGreenĀ® Dye is currently the only qPCR dye to be used in droplet digital PCR (ddPCR).
EvaGreenĀ® Dye has been applied in numerous other applications, such as FluidigmĀ® microfluidic PCR systems, isothermal amplification, capillary gel electrophoresis, DNA quantitation in solution and selective detection of dead cells in cell viability tests. Biotium offers EvaGreenĀ® stand-alone dye, EvaGreenĀ® qPCR master mixes, and other reagents and kits for qPCR.
Biotium implements a Quality System, certified by QAS according to Standard QAS ISO 9001:2015. Certificate No. US3564
All payment in US dollars must be payable on a US bank.
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GelRedĀ® & GelGreenĀ®
|
EMBER500ā¢ RNA Stain
DNAzureĀ® Visible DNA Stain
EvaGreenĀ® Dye
|
Cheetahā¢ Taq
Forget-Me-Notā¢ qPCR Master Mixes
EMBER500ā¢ RNA Stain
|
AccuBlueĀ® RNA Quant
|
RNAstormā¢ RNA Extraction
EverBriteā¢ Mounting Medium
Super-Resolution Microscopy
Tyramide Signal Amplification
AccuGreenā¢ DNA for QubitĀ®
|
AccuClearĀ® DNA
AccuBlueĀ® RNA
Cell Quantitation
|
Live & Dead Stains
NucViewĀ® Caspase-3 Substrates
Nuclear Stains
|
MitoViewā¢ Dyes
Membrane/Cell Surface Stains
ExoBriteā¢ EV Membrane Stains
ExoBriteā¢ Antibody Conjugates
Reactive Dyes
|
Bioconjugates
Near-IR CFĀ® Dyes
Protein Labeling Kits
|
Small Ligand Labeling
Mix-n-Stainā¢ Antibody Labeling Kits
Primary Antibodies
|
Secondary Antibodies
Search with Antibody Finder
IF Background Suppressor
|
WB Blocking Buffer
Autofluorescence Quencher
ViaFluorĀ® Cell Proliferation
|
Live-or-Dyeā¢ Viability
|
Dyes for Spectral Flow
Firefly Assay
|
Renilla Assay
|
Steady-Lucā¢
One-Step Protein Gel Stains
|
Western Blotting
GloMeltā¢ Protein Thermal Stability Assay
RNAstormā¢ FFPE
|
DNAstormā¢ FFPE
Bacterial Stains
|
Yeast Stains
PMA & PMAxxā¢ v-PCR Dyes
|
v-PCR Starter Kits
Strain-Specific Kits
EVAGREENĀ® DYE
For qPCR & Other Applications
Brighter and less inhibitory than SYBRĀ® Green
Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain
The only dye that allows you to perform qPCR and high-resolution melt (HRM) analysis in the same reaction
EvaGreenĀ® Dye is compatible with a number of digital PCR platforms
EvaGreenĀ® PCR products can be run on a DNA gel and visualized without the need for another gel stain
EvaGreenĀ® Dye is used in all of Biotium's dye-based qPCR master mixes, like the superior Forget-Me-Notā¢ Master Mixes
Environmentally safe
Non-mutagenic, non-cytotoxic and safe to aquatic life for safe handling and easy disposal down the drain.
Superior for qPCR and isothermal amplification
Far brighter than SYBRĀ® Green I for detecting amplification due to its novel ārelease-on-demandā DNA-binding mechanism.
Unrivaled DNA melt curve performance
Low PCR inhibition permits the use of saturating dye concentration for maximal signal and high-resolution DNA melt analysis.
Extremely stable
Stable during storage or under PCR conditions.
Serves both as a qPCR dye and a DNA gel stain
After the PCR reaction, directly visualize the PCR product on a gel using a UV transilluminator or blue light box without the need for another gel stain.
Compatible with multiplex PCR
Lack of dye migration from amplicon to amplicon enables detection of multiple PCR products by melt curves.
Useful for other applications
Currently the only qPCR dye to be used in droplet digital PCR (ddPCR). EvaGreenĀ® can also be used for isothermal amplification, microfluidic PCR systems, capillary gel electrophoresis, and more.
Figure 3. A comparison of the raw fluorescence signal from qPCR reactions performed with two EvaGreenĀ® master mixes (Forget-Me-Notā¢ EvaGreenĀ® and Fast EvaGreenĀ®) and QuantiNova SYBRĀ® Green. EvaGreenĀ® Dye is less inhibitory than SYBRĀ® green, allowing for a much brighter signal.
Figure 4. EvaGreenĀ® Dye is safer than SYBRĀ® Green I due to the fact that it is cell membrane impermeable, and therefore cannot gain access to the DNA in the nuclei of living cells.
*Performing HRM may require a license from Idaho Technology.
From: $ 92
Sizes: Trial size (1 mL), 5 x 1 mL Catalog #: 31077-T , - 31077 View all Hide
From: $ 49
Sizes: Trial size (1 mL), 5 x 1 mL Catalog #: 31000 , - 31000-T View all Hide
Forget-Me-Notā¢ EvaGreenĀ® qPCR Master Mix (Low ROX or High ROX)
From: $ 63
Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions) Catalog #: 31045-1mL , 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mL View all Hide
Forget-Me-Notā¢ EvaGreenĀ® qPCR Master Mix (2-Color Tracking)
From: $ 66
Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions) Catalog #: 31041-T , 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mL View all Hide
EvaGreenĀ® Dye is a next-generation DNA-binding dye that is ideal for quantitative real-time PCR (qPCR) and many other applications. Biotium scientists designed the dye by taking into consideration several essential dye properties relevant to PCR, including PCR inhibition, safety, stability and fluorescence spectra of the dye. The result of our efforts is a dye superior to SYBRĀ® Green I and other commercial PCR or high-resolution melt curve (HRM) dyes.*
EvaGreenĀ® Dye is used in all of Biotiumās dye-bas
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