Equine sperm breeding dose calculations
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Semen Extender Calculator - Equine Reproduction
What Exactly is a Dose of Frozen Semen?
PDF How to Process High- and Low-Quality Semen for Cooling and . . .
ARS - Breeding with Cooled Semen
Morphologic assessment of stallion sperm (Proceedings)
PDF BREEDING WITH COOLED SEMEN - College of Veterinary . . .
Centrifugation Protocol : Breeder's Choice, Your Source . . .
Maximizing Pregnancy Rates with Shipped Semen | AAEP
ANS3319C Lab 7: Semen Cryopreservation Flashcards | Quizlet
Some Tips and Discussion about Centrifuging Semen
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www .equine-reproduction .com/SemenCalculator .shtml
Innovative Products Developed Especially for your Horses! Safe, affordable breeding stocks! Saving Foals from FPT Egg Tech - serving European breeder's needs! International Veterinary Information Service . Semen extending calculator Some of the entry fields here are optional, and the page can be used to only calculate doses, or to allow creation . . .
info .selectbreeders .com/blog/bid/151607/What-Exactly-is-a-Dose-of-Frozen-Semen
The total number of sperm in the breeding dose can be calculated by multiplying the sperm concentration by the volume of semen in the breeding dose . Let's take the following as an example: A breeding dose of 8 x 0 .5mL straws consists of a total of 800 million sperm and a post-thaw progressive motility of 35% .
Sperm concentration per milliliter and progres- sive motility are used to calculate a dose of 1 billion progressively motile sperm for shipment . The fol- lowing guidelines can be used to easily calculate the correct volume of semen that should be extended and shipped to ensure that 1 billion progressively motile sperm are contained in each dose .
Stallion owners may ship either one or two doses of semen, each initially containing approximately 1 billion progressively motile spermatozoa (pms) at a concentration of 25 to 50 million pms/ml . Once the shipment arrives, a dose is removed from the shipping container, gently mixed and inseminated directly into the mare without pre-warming .
Sperm quality is the culmination of a variety of factors such as the total sperm number, sperm motility, sperm morphology, and sperm fertilizing ability . Presently in an assessment of the quality of a stallion's ejaculate, it is often based only on the volume, motility, and concentration of the sperm . These are relatively easy parameters to quantify .
csu-cvmbs .colostate .edu/Documents/learnmares1-breed-cooledsem-2009 .pdf
cooled-transported semen has become a routine procedure in the equine breeding industry . The ability to cool semen makes it feasible to ship semen from one location to another, giving mare and stallion owners more flexibility in their breeding programs . In this month's column we will review guidelines for optimizing the use of cooled semen .
Pour off supernatant from each tube to 5-10 ml . and re-suspend pellet of sperm by gently agitating . Add enough extender to bring concentration of progressively motile cells to 25-50 million/ml . Use worksheet to calculate volume of extender . Divide volume by number of centrifuge tubes used .
Standards for a adequate breeding dose have not changed since the early 1980's when Householder et al reported that the minimum insemination dose for optimal pregnancy rate contained 500 million progressively motile spermatozoa (PMS) inseminated every other day while a mare was in heat .
volume and target sperm concentration of single does of bovine semen to be frozen .25 or .5 mL; 20x10^6 live, motile sperm/dose reason semen for freezing must be high quality and have more cells packaged per straw than needed many sperm cells die during the freezing/thawing process
Ideally one ships an insemination dose of 1 billion progressively motile sperm (at the time of shipment) which, allowing for an arbitrary 50% die-off rate, provides the desired insemination dose of 500 million progressively motile sperm . In order to achieve a successful shipment of fertile sperm however other parameters must be considered .
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Equine sperm breeding dose calculations
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