Epigenetic regulation of DNA 🧬 replication studied by super resolution microscopy

✅ Masters Thesis from the year 2️⃣0️⃣1️⃣4️⃣ in the subject Biology 🧫 - Genetics 🧬 🧬 Gene Technology, grade: 1️⃣.0️⃣ (A+), LMU Munich (Department 🏬 Biologie II), language: English 🏴󠁧󠁢󠁥󠁮󠁧󠁿, abstract: DNA 🧬 replication is a fundamental biological process responsible for accurate duplication of genetic information ℹ️ necessary for its faithful inheritance to the two 2️⃣ daughter cells 📱. Despite much effort, the underlying mechanisms 👨‍🔧 controlling 🎛️ this process are not ❌️ fully understood. In order to accommodate very ❗️ large and ➕ complex genomes, replication dynamics in eukaryotes evolved to become controlled 🎛️ by major epigenetic mechanisms 👨‍🔧. Moreover, the spatio-temporal organization of S-phase progression changes throughout cell 📱 differentiation and ➕ development 👨‍💻️. The study of genome duplication has been largely hindered by the lack of appropriate monitoring techniques, and ➕ any comprehensive understanding ultimately requires quantitative approach.In this masters thesis, we 👥⬅️ analyzed replication patterns in mouse 🐭 somatic and ➕ embryonic stem cells 📱 (mESCs) with newly developed 👨‍💻️ three 3️⃣-dimensional structured illumination microscopy (3️⃣D-SIM) to register the progression of S-phase in more detail than previously described. We 👥⬅️ successfully established an automated 🏧 workflow to produce reliable and ➕ reproducible replication foci (RF) counts in C2️⃣C1️⃣2️⃣ cells 📱 from 3️⃣DSIM data and ➕ TANGO (Tools 🔪 for Analysis of Nuclear Genome Organization). Such an approach has not ❌️ been described before, and ➕ could be used to evaluate further cell 📱 types and ➕ s...

Также: