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Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins.

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Official websites use. Share sensitive information only on official, secure websites. The Saccharomyces cerevisiae CDC25 gene and closely homologous genes in other eukaryotes encode guanine nucleotide exchange factors for Ras proteins. We have determined the minimal region of the budding yeast CDC25 gene capable of activity in vivo. The region required for full biological activity is approximately residues and contains two segments homologous to other proteins: one found in both Ras-specific exchange factors and the more distant Bud5 and Lte1 proteins, and a smaller segment of 48 amino acids found only in the Ras-specific exchange factors. To more directly study the physical interaction of CDC25 and Ras proteins, we developed a protein-protein binding assay. We determined that CDC25 binds tightly to Ras2 protein only in the absence of guanine nucleotides. This higher affinity of CDC25 for the nucleotide-free form than for either the GDP- or GTP-bound form suggests that CDC25 catalyzes exchange of guanine nucleotides bound to Ras proteins by stabilization of the transitory nucleotide-free state. These references are in PubMed. This may not be the complete list of references from this article. As a library, NLM provides access to scientific literature. Mol Cell Biol. Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins. Find articles by C C Lai. Find articles by M Boguski. Find articles by D Broek. Find articles by S Powers. PMC Copyright notice. Full text PDF. Previous … Next. Image on p. Similar articles. Add to Collections. Create a new collection. Add to an existing collection. Choose a collection Unable to load your collection due to an error Please try again. Add Cancel.

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