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My Library. You currently do not have any folders to save your paper to! Create a new folder below. Create New Folder. Folder Name. Folder Description. Arachnologische Mitteilungen: Arachnology Letters Vol. High Atlas. Subscribe to BioOne Complete. Receive erratum alerts for this article. Receive alerts when this article is cited. Citation Only. Help Advanced Search. All Titles. Choose Title s. Single Year. Clear Form.

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Official websites use. Share sensitive information only on official, secure websites. The common sowthistle, Sonchus oleraceus Asteraceae , is a globally invasive weedy species. In order to investigate its genetic diversity, population genetic structure, and evolutionary history, we developed and characterized nuclear simple sequence repeat markers SSRs or microsatellites. Seventeen microsatellite primer pairs were developed based on the Illumina sequence data. Ten developed SSR loci were polymorphic in four populations sampled from broad geographical regions. The number of alleles per locus ranged from one to 11, and the levels of observed and expected heterozygosity ranged from 0. The SSR markers developed in this study will be useful for future population genetic studies on S. Keywords: Asteraceae, invasive species, microsatellite markers, Sonchus oleraceus , weeds. The common sowthistle, Sonchus oleraceus L. Asteraceae , is an annual or biennial herb species. As one of the cosmopolitan weed species in the genus Sonchus L. Sonchus oleraceus and S. In addition, the evolution of herbicide resistance found in populations of S. Although S. Seashore habitats in the Mediterranean region have been proposed to be the geographical origin of the common sowthistle Boulos, , although this is not confirmed. Stebbins et al. Therefore, in order to infer its phylogenetic divergence and expansion history on a local and global scale, it is of great importance to determine the origin of amphidiploid S. However, to our knowledge, no microsatellite markers have been developed for S. In this study, we developed 10 polymorphic and seven monomorphic nuclear simple sequence repeat SSR markers for S. In addition, we tested their applicability in four congeneric taxa S. Ball, S. Boulos, and S. Our results suggest that the developed SSR markers will be valuable for future population genetic studies of Sonchus species. Two individuals of S. Sonchus asper subg. Sonchus L. Dendrosonchus Sch. Altogether, Microsatellite motifs with a repeat unit ranging from two to six nucleotides and a minimum number of six repeats were detected using MISA version 1. A total of regions were found. Primer pairs were designed using Primer3 version 2. Characteristics of 17 microsatellite markers developed from Sonchus oleraceus genomic sequences. Although most of the samples were amplified based on a higher T a setting, some samples failed to amplify, and thus, lower annealing temperatures were used for PCR. Of the regions identified, randomly selected primer pairs were tested for amplification efficiency by PCR using eight representative individuals of S. Of these primer pairs, 33 failed to amplify and the remaining 74 displayed clear bands and were suitable for further analysis. The microsatellite marker profiles were scored using GeneMapper version 5. Of the 74 successfully amplified loci, 10 were found to be polymorphic and seven were monomorphic Table 1. The remaining 57 loci were excluded due to difficulty in obtaining clean peaks and unsuccessful amplification after labeling the forward primer with fluorescent dye. Population genetic parameters, including the number of alleles, expected heterozygosity, and observed heterozygosity, were calculated using GenAlEx version 6. Polymorphism information content, a value that is indicative of a measure of the informativeness of a genetic marker for linkage studies, was estimated using CERVUS version 3. In four investigated populations of S. Significant deviation from Hardy—Weinberg equilibrium was detected in the four populations: Australia eight loci , Germany three loci , China two loci , and Korea one locus Table 2. Genetic properties of 17 newly developed microsatellite markers in four populations of Sonchus oleraceus in Eurasia and Australia. Locality and voucher information are provided in Appendix 1. Out of 17 successfully amplified loci, six and seven loci were found to be polymorphic in S. Furthermore, out of 14 successfully amplified loci in two woody Sonchus species S. In this study, we developed the first set of microsatellite markers for the genus Sonchus. Ten polymorphic markers are suitable for evaluating the population genetics and evolutionary history of S. All authors approved the final version of the manuscript. GenBank accession numbers are provided in Table 1. Kim, S. Development and characterization of 17 microsatellite markers for Sonchus oleraceus. Applications in Plant Sciences 8 3 : e This section collects any data citations, data availability statements, or supplementary materials included in this article. As a library, NLM provides access to scientific literature. Appl Plant Sci. Open in a new tab. Locus S. Species Collection locality Geographic coordinates N Voucher specimen accession no. Similar articles. Add to Collections. Create a new collection. Add to an existing collection. Choose a collection Unable to load your collection due to an error Please try again. Add Cancel. Sonchus asper subsp.

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