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This event is free. Volunteer at this Event. Join us on the San Francisco waterfront to tour our visiting ships docked at the Port of San Francisco and get a glimpse of the day-to-day lives of the sailors serving in the U. Navy and U. Coast Guard. We have several guides on this site to help you with information about planning your experience at Fleet Week under 'Plan Your Trip'. It is the fourth United States Navy vessel and the second warship to bear this name, the first two being a wooden-hulled motorboat and a ferry. The modern ship was named specifically for Somerset County Pennsylvania , in honor of the passengers who died on United Airlines Flight 93 , which was hijacked during the terror attacks of 11 September The passengers prevented the plane from reaching its intended target by forcing it to crash in Stonycreek Township in Somerset County, Pennsylvania. The words ' Let's Roll ,' spoken by a passenger of United Airlines Flight 93 before trying to storm the cockpit of the doomed flight, are painted on the ship above the rear deck, along with a '93' seal which mirrors the '93' flag seen flying on the vessel in many photos. In the words of Secretary of the Navy Gordon R. England ;. She is named for Commodore Ellsworth P. She was launched on September 29, , christened November 11, , and commissioned on August 4, The cutter's home port is Alameda, California. Bertholf was the first to fire the Bofors 57 mm gun aboard a U. Regina is the fifth vessel in her class which is the name for the Canadian Patrol Frigate Project. The public is encouraged to attend tours and interact with U. Navy, Marine Corps and Coast Guard personnel. Ship Tours. October 9 - 14 Varies, See Below. October 11 No Ship Tours. Come climb aboard the amphibious carriers, cutters, destroyers and cruisers docked at the Embarcadero and get a glimpse into the day-to-day lives of the sailors serving in the U. Navy, and the U. All ship tours are free of charge. Tour lines are on a first-come, first-served basis. Download our app for updates:. Review Ship Tour Code of Conduct. England ; 'The courage and heroism of the people aboard the flight will never be forgotten and USS Somerset will leave a legacy that will never be forgotten by those wishing to do harm to this country. Conduct During Shipboard Tours. Report any suspicious activity immediately to military or police personnel Over 18 years of age must have a valid government-issued ID Those 17 years old and under must be accompanied by an adult. No large backpacks, oversized camera bags, or large purses No holding areas for personal items - backpacks, strollers, bicycles, scooters, pets, etc. All non-permitted items will not be allowed to remain on or near the pier areas. They will be removed from the pier and may be searched or otherwise disposed of. No pre-registration or RSVP is required Visitation to all participating vessels is on a first-come, first-served basis. Ship tours may not be safe for those with some disabilities and access and functional needs or certain medical conditions. There are steep ladders stairways to climb and uneven surfaces to traverse on board the ship. An alternative experience will be provided on the pier for those who wish to learn more about the ships in Port. Open-toe shoes are not recommended. High heels are not permitted. Failure to consent to screening and inspection will result in denial of authorization to enter the property. All visitors will remain with the tour guide at all times and will not deviate from the dedicated tour route. Report any suspicious activity immediately to any military or police personnel. Prohibited Items All personnel are screened, and all bags are searched before entering the pier.
Down modulation of IFN-γ Signaling in Alveolar Macrophages Isolated from Smokers
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Official websites use. Share sensitive information only on official, secure websites. Email: ALadner kumc. This included a decrease in the phosphorylation of signal transducer and activator of transcription STAT -1 and induction of interferon regulatory factor IRF Smoking-related morbidity encompasses many organ systems, but none more so than the lung. The acute and chronic impairment of immune function associated with smoking has been well described, yet there continues to be no cohesive model that explains precisely how smoking causes disease within the human lung. Multiple defense mechanisms, directed against infection and malignancy, are operative throughout the human lung, with the alveolar macrophages AM playing a sentinel role in immune surveillance within the alveolar space. However, significant changes have been found to occur in AM isolated from smokers, when compared with those isolated from non-smokers Harris et al. The numbers of AM have been reported to increase in smokers Barbers et al. In addition, previous studies from our lab and by others on AM from smokers suggest attenuated secretion of IL-1 Brown et al. This cytokine possesses a wide spectrum of biological effects and is crucial for development of the highly activated macrophage phenotype characteristically found during inflammation Murray, ; Williams et al. This observation may have widespread significance with respect to infection, the development of cancers, and the treatment of such, in the lungs of smokers. Eight healthy, smoking volunteers and four nonsmoking volunteers underwent bronchoalveolar lavage for the isolation of AM. Mean ages were None of the subjects used for this study were taking medication, had a history of pulmonary disease, or had a recent upper respiratory tract infection. Subjects were given physical exams, each of which proved normal, and no subject showed evidence of lung disease, as judged by pulmonary function tests. Cell viability was determined by trypan blue exclusion, and cells were counted in a hemocytometer. A differential cell count and purity of AM was determined using a cytospin slide preparation stained with Diff-Quik. Approximately 2. AM isolated from either smokers or from non-smokers, had no difference in their ability to adhere to the surface. After 1 hour adherence to assure macrophage purification , AM isolated from both smokers and non-smokers, were used for soluble extract SE preparation. Extracts were prepared as described by Gao et al Gao et al. The secondary antibodies used were horseradish peroxidase-conjugated anti mouse or anti rabbit , Pierce Chemical Co. Where appropriate, membranes were stripped, reblocked, and sequentially reprobed with subsequent antibodies. In some experiments, SE were first treated with peptide N- glycosidase F PNGase F prior to western blot analysis to remove N-linked oligosaccharides from the peptide backbone Fischer et al. Breifly, cellular lysate was denatured by boiling for 10 min in extraction buffer supplemented with 0. Statistical analysis was performed using one-way or two-way analysis of variance with a post hoc Student t test. The receptor protein displayed a diffuse banding pattern due to the heterogeneous addition of carbohydrate moieties to the polypeptide backbone. The same relative molecular mass is indicated by the arrowhead in the untreated samples top panel. Statistical significance was calculatedusing a two-tail, independent, t-test. Further, to confirm the cell surface nature of the broad bands detected in Figure 1 A , and to sharpen the resultant banding pattern, we treated protein extracts with PNGase F prior to western blot analysis. This protein is also very heterogeneous in size and was difficult to detect in western blots of extracts, not digested with PNGase F. However, as shown in Figure 1C , a protein band of appropriate size 38kDa , was observed in PNGase F-treated samples that was not found in untreated samples. We found 2. Following treatment, whole cell extracts WCE were prepared and then analyzed by western blot. Because the appearance of newly induced IRF-1 requires both de novo gene transcription and protein synthesis, there is little additional IRF-1 protein detected after 0. When blots of the protein extracts from AM of smokers and non-smokers were probed with anti-SOCS-1 antibody, no protein was detectable in either the non-smokers or smokers group data not shown , although the SOCS-1 band did appear in the tissue extract from mouse brain used as a positive control. As a positive control of SOCS-3 antibody binding, a cellular extract was prepared from mouse brain see the Materials and Methods and electrophoresed alongside the AM samples lane C. However, previous studies by Celada et al. HuangFu et al. Furthermore, AM isolated from 3-of-7 lung cancer patients, in the study of Halme et al. Halme et al. Clinical trials, utilizing an intravenous Schiller et al. The authors dedicate this paper to the fond memory of Joseph C. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. As a library, NLM provides access to scientific literature. Toxicol Appl Pharmacol. Published in final edited form as: Toxicol Appl Pharmacol. Find articles by Navneet K Dhillon. Find articles by William J Murphy. Find articles by Michael B Filla. Find articles by Ana J Crespo. Find articles by Heath A Latham. Issue date May PMC Copyright notice. The publisher's version of this article is available at Toxicol Appl Pharmacol. Open in a new tab. Conflict of Interest statement The authors declare that there are no conflicts of interest. Similar articles. Add to Collections. Create a new collection. Add to an existing collection. Choose a collection Unable to load your collection due to an error Please try again. Add Cancel.
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Down modulation of IFN-γ Signaling in Alveolar Macrophages Isolated from Smokers
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Down modulation of IFN-γ Signaling in Alveolar Macrophages Isolated from Smokers
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