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Описывается новый ме тод дифференциально й окраски активных и инактивны х с точки зрения метаболизма м икробов, — как исходно й работы при определении условий их роста, устойчивости, влияни я на них лекарственны х и дезинфекционных сре дств. Метод заключает ся в окраске незафиксир ованных бактерий мет иленовым синим при последующе м окислении с помощью красной кро вяной соли или переки си водорода и докраске основным ф уксином. С помощью этого метод а бактерии распредел яются на 2 группы: на двоякоокра шивающуюся группу синезеленым и даже черным у клеток с активным метаболизмом и красн ым у инактивных клеток и на группу, у ко торой это явление не наблюдается. Мы установили, что инактивность не д олжна обязательно означат ь смерть микрооргани зма. A description is given of a new method of staining, for differentiating metabolically active from inactive micro-organisms, as the basis for resolving the conditions of their growth and resistance and the influence of drugs and disinfectants. The method consists in staining non-fixed bacteria with methylene blue, then in oxidation with potassium ferrocyanide or hydrogen peroxide and in staining finally with basic fuchsin. By using this method the bacteria are divided into two groups, a group in which double staining occurs bluish-green to blue in metabolically active bacteria and red in inactive and a group in which this does not occur. The method was verified by studying the influence of certain physical and chemical factors on the bacteria before using the method, by ascertaining the relationship of the microorganisms staining bluish-green and red to their ability to grow, by observing staining of the micro-organisms at various stages of their growth and by establishing an index the proportion of bluish-green staining to red staining organisms , and by ascertaining the relationship bofh forms to the living organism and to mortality in mice. It was found that inactivity does not of necessity mean the death of the micro-organism. This is a preview of subscription content, access via your institution. Rent this article via DeepDyve. Clark, W. Treasury Dep. Public Health Service. Washington Google Scholar. KU Praha Ried, W. Chemie , Strugger, S. Jena 97, CAS Google Scholar. Download references. You can also search for this author in PubMed Google Scholar. Reprints and Permissions. Download citation. Received : 29 November Issue Date : July Search SpringerLink Search. Souhrn 1. Резюме Описывается новый ме тод дифференциально й окраски активных и инактивны х с точки зрения метаболизма м икробов, — как исходно й работы при определении условий их роста, устойчивости, влияни я на них лекарственны х и дезинфекционных сре дств. Summary A description is given of a new method of staining, for differentiating metabolically active from inactive micro-organisms, as the basis for resolving the conditions of their growth and resistance and the influence of drugs and disinfectants. Immediate online access to all issues from Subscription will auto renew annually. Literatura Clark, W. Google Scholar Ried, W. View author publications. MUDr J. Rights and permissions Reprints and Permissions.
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