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Federal government websites often end in. The site is secure. Preview improvements coming to the PMC website in October Learn More or Try it out now. In rodents, cholinesterase inhibitors can cause sustained decreases in the reinforcing effects of cocaine. Nonetheless, cocaine is metabolized by butyrylcholinesterase BuChE , raising concerns that cholinesterase inhibition could increase its peripheral concentrations, perhaps augmenting toxicity. Although donepezil is approved for use in patients and selective for inhibiting acetylcholinesterase over BuChE, no studies have reported cocaine bioavailability in human subjects receiving donepezil. Twelve cocaine-dependent veterans received three days of treatment with either oral placebo or 5 mg daily of donepezil, followed by cross-over to the opposite treatment. During both oral treatments, double-blind intravenous cocaine was administered at 0. Intravenous cocaine produced dose-related increases in systolic blood pressure that were most pronounced over the initial 30 minutes after treatment. Oral donepezil attenuated drug-induced elevations of systolic blood pressure following low-dose cocaine 0. No significant difference in blood pressure following treatment with placebo or donepezil after high-dose cocaine 0. Peak values of blood pressure and heart rate were unaffected by donepezil. Plasma concentrations of cocaine and metabolites did not differ in donepezil- and placebo- treated participants. We conclude that donepezil can attenuate drug-induced increases in systolic blood pressure following low-dose cocaine, but does not otherwise modify the cardiovascular effects of intravenous cocaine. Clinically significant changes in cocaine bioavailability and cardiovascular effects do not occur following this dose of donepezil. Cocaine is an addictive stimulant that can cause psychiatric disorders, seizures, stroke, and cardiovascular toxicity. Correcting for age and demographics, cocaine-induced mortality was associated with an increased incidence of left ventricular hypertrophy, ischemic heart disease, and aortic or cerebrovascular atherosclerosis. There is evidence that cocaine has pro-thrombotic properties that may promote cardiac or brain ischemia. The neurotransmitter acetylcholine plays a key role in drug reinforced behavior. Cocaine reinforcement activates cholinergic neurons in the nucleus accumbens, with the number of activated cells in the nucleus accumbens shell region correlated with the amount of drug self-administered. The physiologic role of BuChE is unclear, but it can metabolize cocaine and other exogenous compounds and appears to contribute to degradation of acetylcholine. Animal studies have also shown concentrations of donepezil in brain exceed values in plasma by more than six-fold, 15 but comparable human data are not available. Pretreatment with donepezil and other cholinesterase inhibitors can attenuate cocaine reinforced behavior in non-human primates, rats, and mice. In addition, the safety of cholinesterase inhibitors remains an important issue. Given the high rate of relapse in treatment-seeking individuals, it is assumed that cholinesterase inhibitors will be coadministered with cocaine in a significant number of patients. A medication that produced greater availability of cocaine through BuChE inhibition could potentiate cocaine-induced brain and cardiac toxicity. Both cocaine and cholinergic agents can have pronounced effects on the autonomic nervous system, increasing the likelihood of drug interactions. The present study was conducted to examine the effects of donepezil pretreatment on cardiovascular measures, plasma concentrations of cocaine, and cocaine metabolites. Detailed methods and changes in the subjective effects of cocaine after donepezil treatment in this trial have been previously reported. Prior to enrollment, participants were medically screened by physical and mental health exams performed by a physician and routine chemistry, blood-count, and urinalysis studies. All participants met criteria for cocaine dependence, according to the Diagnostic and Statistical Manual of Mental Disorders, 4th text revision edition. An initial session was performed to ensure that participants tolerated intravenous cocaine. Two subsequent sessions were conducted three hours following a third daily dose of donepezil 5 mg or oral placebo. The different oral treatments were separated by a three-day washout period. Each laboratory session included four injections administered at one-hour intervals, two of intravenous placebo, one of low-dose cocaine, and one of high-dose cocaine 0. To mitigate potential toxicity when combined with donepezil, the low and high doses of cocaine were always administered in an ascending order, separated by an injection of intravenous placebo. This ensured that patients tolerated low-dose cocaine combined with donepezil, before being advanced to high-dose cocaine with donepezil. Because of the expense of assays, plasma samples for determination of cocaine and metabolites were only collected after high-dose cocaine. To maintain the blind, blood samples were collected after both high-dose cocaine and its corresponding intravenous placebo. During double-blind oral dosing, participants were randomized in equal numbers to receive intravenous treatments in one of two treatment orders: placebo, 0. A gauge intravenous catheter was inserted into the antecubital fossa on the dominant side for administration of cocaine and blood sampling. Saline or cocaine was administered by intravenous push over two minutes, with a physician present. Study procedures were reviewed and approved by the Kansas City VA Human Subjects Committee, with all subjects providing written informed consent. Healthy, regular users of cocaine between 18 and 50 years of age were included. All participants were male, with a mean age of On average, they reported using cocaine on Participants reported having consumed alcohol for an average of In order to participate, they were required to provide a recent urine sample that tested positive for the presence of benzoylecgonine. Only individuals who were not actively seeking medical treatment for cocaine dependence were included. Those with a history of a medical adverse reaction to cocaine, a current psychiatric disorder other than drug abuse, or unstable medical condition were excluded. Additional demographic detail on the study participants has been previously reported. Blood pressure and heart rate were recorded at the midpoint and completion of each intravenous infusion; and at 4, 6, 8, 10, 15, 20, 30, 40, and 50 minutes after dosing. Participants received an initial 0. Two hours after the first injection, a second 0. Plasma determinations of cocaine, benzoylecgonine, ecgonine methyl ester, and norcocaine were made at baseline and 10, 15, 30, 60, , , and minutes after the start of the second intravenous injection. Blood was collected into Vacutainer tubes containing additives to inhibit hydrolysis of cocaine by plasma cholinesterases Becton Dickinson catalog number , 4 ml aliquots of blood were each collected into tubes containing 10 mg of sodium fluoride and 8 mg of potassium oxalate. Plasma levels of cocaine and metabolites were assayed by liquid chromatography-tandem mass spectrometry with a lower limit of quantitation of 2. Analysis of variance ANOVA was performed using oral dose placebo or 5 mg daily of donepezil and intravenous dose placebo, low-dose cocaine, and high-dose cocaine as factors. Post hoc contrasts were accomplished by either additional ANOVAs, or through t -tests after applying the correction by Sidak. The pharmacokinetic parameters maximum plasma concentration C max , and time to reach C max T max were determined from inspection of the data. A non-compartmental model was used to fit all plasma concentration versus time data. Plasma concentrations of cocaine and metabolites were also compared by analysis of variance, using all available data points. Neither baseline blood pressure nor heart rate differed after participants received three days of oral placebo or donepezil Table 1. Change in systolic blood pressure over time after different treatments is shown in Figure 1. Intravenous cocaine produced expected dose-related increases in systolic blood pressure that were most pronounced over the initial 30 minutes after treatment. Diastolic blood pressure and heart rate also showed trends for cocaine-induced increases, but were unaffected by donepezil treatment data not shown. Time-course of systolic blood pressure following intravenous injections of placebo or cocaine. Vertical axes show absolute change from baseline recorded in 12 participants at different time points. Group means and standard error are shown after treatment with intravenous placebo, low-dose cocaine 0. Cocaine produced dose-related increases in systolic blood pressure, which were attenuated in donepezil-treated participants receiving low-dose cocaine. Cardiovascular measures after pretreatment with oral placebo or donepezil, prior to intravenous injections. Data shows group means in 12 subjects after receiving three days of either treatment, with standard error. Maximum or peak values for blood pressure and heart rate after different treatments are shown in Figure 2. Oral donepezil treatment did not modify peak values for any of the cardiovascular measures. Relative to treatment with intravenous placebo, high-dose cocaine increased all three peak measures combined across both oral treatments. Peak values for cardiovascular measures following intravenous injections of cocaine. Vertical axes show group means for maximum values of heart rate, diastolic blood pressure, and systolic blood pressure for 12 participants. When collapsed across oral treatment, high- but not low- dose cocaine produced significant elevations in all three measures. Prior to receiving a second cocaine infusion two hours after an initial dose of cocaine for that session , cocaine was detectible in plasma from all except one placebo-treated subject, and two donepezil-treated subjects. All participants had detectible concentrations of cocaine in plasma collected between 10 and minutes after intravenous dosing. At the four hour-time point, plasma cocaine was detectible in five subjects treated with oral placebo and five subjects treated with donepezil. None of the subjects evaluated had detectible plasma concentrations of cocaine at the eight-hour time point. Group means for plasma concentrations of cocaine after oral placebo or donepezil treatment are shown in Figure 3B. All participants had plasma cocaine detectible at a sufficient number of time points to derive pharmacokinetic parameters. Visual inspection and curve fitting of log-transformed plasma concentrations of cocaine suggested a one-compartment disposition in some subjects, with data from other participants more consistent with a two-compartment model. Neither a single- nor dual- compartment model was adequate to account for data from all participants. Accordingly, a pure non-compartmental analysis was used to derive pharmacokinetic parameters, which are shown in Table 2. All subjects exhibited a time of maximal concentration T max of 10 minutes. For pharmacokinetic parameters, we observed substantial inter- and intra- individual across session variation. Donepezil treatment did not alter plasma concentrations of cocaine, benzoylecgonine, or ecgonine methyl ester; or any of the derived pharmacokinetic parameters. For values combined across both oral treatments, plasma benzoylecgonine was increased for all time points after dosing with high-dose cocaine, with cocaine and ecgonine methyl ester increased for all time points up to 2 and 4 hours following dosing, respectively. Plasma concentrations of cocaine and major metabolites following oral donepezil. Group means are shown for individual subjects with detectible levels see text for details. Twelve participants received an initial injection of 0. Concentrations of cocaine and metabolites were only determined after the second injection of cocaine. No significant effects of cholinesterase inhibition were observed. Pharmacokinetic measures in subjects receiving oral placebo or donepezil. Non-compartmental analysis was used to derive measures from each individual subject, with mean values and standard error shown. Plasma concentrations of cocaine metabolites are shown in Panels A and C of Figure 3 , with pharmacokinetic parameters reported Table 2. As for the parent cocaine molecule, neither concentrations of benzoylecgonine nor ecgonine methyl ester differed after donepezil treatment. Benzoylecgonine was detectable in plasma at all time points in both placebo- and donepezil treated subjects. Because less than three values were available in its terminal phase, elimination rate constants could not be calculated in two placebo and three actively-treated participants. Benzoylecgonine was eliminated with a half-life of approximately six hours, which was unaffected by donepezil treatment. Prior to the second cocaine infusion, ecgonine methyl ester was detectible in plasma from seven placebo-treated and five donepezil-treated subjects. After the second cocaine infusion, ecgonine methyl ester was detectible in plasma from most subjects, with no differences in the number of detectible samples in placebo- and donepezil- treated subjects. Because less than three values were available in its terminal phase, elimination rate constants could not be calculated in three placebo and four actively-treated participants. Ecgonine methyl ester was eliminated with a half-life of approximately five hours, which was again not altered by donepezil treatment. Norcaine, a minor metabolite of cocaine, was not detected in plasma from any subject. This study shows that patients who received donepezil prior to a low dose of intravenous cocaine exhibited lower drug-induced increases in systolic blood pressure. In contrast, increases in blood pressure after treatment with high-dose cocaine were not affected by donepezil treatment. Heart rate and diastolic blood pressure were unaffected by either dose of cocaine. Plasma concentrations of cocaine and major metabolites also did not differ in subjects receiving either donepezil or oral placebo. The pattern of metabolites matches previous reports of intravenously administered cocaine. Despite a large number of clinical trials evaluating many potential agents, no medication has been generally accepted or approved by the Food and Drug Administration to assist dependent individuals in avoiding further use of cocaine. As a first step in evaluating a medication to treat cocaine-use disorders in humans, its safety during combined dosing with cocaine and potential pharmacokinetic interactions with cocaine or cocaine metabolites must be assessed. To enhance the safety of human participants, interaction studies should be completed at each dose level before advancing to a higher dose. Otherwise, human subjects might be exposed to an adverse effect that was more intense because of the higher dose administered. At least for the 5 mg daily dose of donepezil, results of the present study do not show adverse cardiovascular effects or unfavorable pharmacokinetic interactions. Cocaine is hydrolyzed by BuChE in the blood and hepatic carboxylesterase 2 to ecgonine methyl ester, and by hepatic carboxylesterase 1 to benzoylecgonine. Because it is the most selective of marketed cholinesterase inhibitors, with fold greater selectivity for inhibition of AChE over BuChE, 27 donepezil is presumably the least likely to augment tissue levels of cocaine through inhibition of BuChE. Results of the present study support this hypothesis. The effect of donepezil on hepatic carboxylesterases has not been reported, but lack of changes in plasma concentrations of ecgonine methyl ester or benzoylecgonine suggest any effect on these esterases is limited. The absence of these effects in experienced cocaine users receiving intravenous cocaine argue against significant enzyme inhibition after treatment with 5 mg daily of donepezil. Nonetheless, greater doses of cocaine with use over a more prolonged periods are likely in a non-laboratory setting. Through increased availability of acetylcholine, cholinesterase inhibition can reduce heart rate by direct cardiac actions at the sinoatrial node, and increase blood pressure through activation of central type 1 and 2 muscarinic receptors. Given the high potential for relapse to use of cocaine, treatment of cocaine-dependent patients with cholinesterase inhibitors requires a careful evaluation of the potential for negative interactions. Cocaine-induced lethality in rats is decreased and increased by low and high doses of the cholinesterase inhibitor physostigmine, respectively. As previously reported, the present study showed that acute dosing with cocaine causes dose-related increases in heart rate and blood pressure. Given the potential of cholinesterase inhibitors to cause hypertension in some instances, an additive interaction between donepezil and cocaine would have augmented cocaine-induced hypertension. Instead, we observed an opposite effect, with donepezil attenuating increases in blood pressure produced by low-dose cocaine. By increasing heart rate and blood pressure, cocaine acutely elevates cardiac workload. These actions may contribute to cardiac ischemia and also jeopardize brain vessels at risk for stroke. Recently, donepezil treatment in a rat cardiac ischemia model was shown to prevent heart failure and improve survival following coronary artery ligation. This parallels positive effects of vagal stimulation in experimental models of cardiac ischemia. Although we did not record sympathetic function, future studies of cocaine interactions with cholinesterase inhibitors should consider this approach. Rather than potentiating cardiovascular events, administration of cocaine with cholinesterase inhibitors may be protective by ameliorating increases in sympathetic tone. This mechanism may explain attenuation of drug-induced increases in blood pressure caused by low-dose cocaine in the current study. There are significant limitations of our study design. Because it is highly protein bound with an elimination half-life of Although the three days of dosing utilized did not accomplish steady-state concentrations, our study design was a tradeoff between the length of confinement through hospitalization and its cost. Donepezil can acutely attenuate cocaine self-administration in rodent studies, 16 and our aim was therefore to evaluate effects of subacute dosing in humans. Because of its potential toxicity, doses of cocaine were chosen to be the minimum that produced reliable subjective effects in a laboratory. Amounts of cocaine used in a non-research setting may be significantly greater. Nonetheless, the higher cocaine dose chosen for the present study is similar to those typically used for human laboratory studies of cocaine. Significant dose-limiting effects of cocaine have been observed after higher doses in this setting. This reflects both individual differences in drug metabolism and the limits of detection by mass spectrophotometry. Although further study is needed, pretreatment with the cholinesterase inhibitor donepezil does not augment cocaine bioavailability after exposure to the cocaine dose typically used in a laboratory setting. Rather than potentiating cocaine-induced increases in blood pressure, donepezil attenuated systolic blood pressure elevation following a low dose of cocaine. Diastolic blood pressure and heart rate were unaffected. Future studies should evaluate higher doses of donepezil for both the potential to decrease the reinforcing effects of cocaine and as a means of diminishing negative cardiovascular consequences. The authors report no conflicts of interest, and they alone are responsible for the content and writing of this paper. As a library, NLM provides access to scientific literature. Am J Addict. Author manuscript; available in PMC Aug 1. Newton , MD, 4 David E. Thomas F. David E. PMC Copyright notice. The publisher's final edited version of this article is available at Am J Addict. Abstract Background In rodents, cholinesterase inhibitors can cause sustained decreases in the reinforcing effects of cocaine. Methods Twelve cocaine-dependent veterans received three days of treatment with either oral placebo or 5 mg daily of donepezil, followed by cross-over to the opposite treatment. Results Intravenous cocaine produced dose-related increases in systolic blood pressure that were most pronounced over the initial 30 minutes after treatment. Conclusions and Scientific Significance We conclude that donepezil can attenuate drug-induced increases in systolic blood pressure following low-dose cocaine, but does not otherwise modify the cardiovascular effects of intravenous cocaine. Introduction Cocaine is an addictive stimulant that can cause psychiatric disorders, seizures, stroke, and cardiovascular toxicity. Methods Design and Participants Detailed methods and changes in the subjective effects of cocaine after donepezil treatment in this trial have been previously reported. Cocaine Plasma Concentrations Participants received an initial 0. Results Cardiovascular measures Neither baseline blood pressure nor heart rate differed after participants received three days of oral placebo or donepezil Table 1. Open in a separate window. Figure 1. Table 1 Cardiovascular measures after pretreatment with oral placebo or donepezil, prior to intravenous injections. Measure Placebo Donepezil Heart Rate beats per minute Figure 2. Cocaine Pharmacokinetic Profile Prior to receiving a second cocaine infusion two hours after an initial dose of cocaine for that session , cocaine was detectible in plasma from all except one placebo-treated subject, and two donepezil-treated subjects. Figure 3. Table 2 Pharmacokinetic measures in subjects receiving oral placebo or donepezil. Cocaine Metabolites Plasma concentrations of cocaine metabolites are shown in Panels A and C of Figure 3 , with pharmacokinetic parameters reported Table 2. Discussion This study shows that patients who received donepezil prior to a low dose of intravenous cocaine exhibited lower drug-induced increases in systolic blood pressure. Footnotes Declaration of Interest The authors report no conflicts of interest, and they alone are responsible for the content and writing of this paper. References 1. Cocaine: history, social implications, and toxicity--a review. Dis Mon. United Nations Office on Drugs and Crime. World Drug Report. Mortality risk factors and excess mortality in a cohort of cocaine users admitted to drug treatment in Spain. J Subst Abuse Treat. Comparative cardiac pathology among deaths due to cocaine toxicity, opioid toxicity and non-drug-related causes. Cocaine cardiotoxicity: a review of the pathophysiology, pathology, and treatment options. Am J Cardiovasc Drugs. Cocaine use and risk of stroke: a systematic review. Drug Alcohol Depend. Cocaine and thrombosis: a narrative systematic review of clinical and in-vivo studies. Subst Abuse Treat Prev Policy. Buttner A. Neuropathological alterations in cocaine abuse. Curr Med Chem. Williams MJ, Adinoff B. The role of acetylcholine in cocaine addiction. Cholinergic interneurons of the nucleus accumbens and dorsal striatum are activated by the self-administration of cocaine. Accumbens dopamine-acetylcholine balance in approach and avoidance. Curr Opin Pharmacol. Cholinesterases: roles in the brain during health and disease. Curr Alzheimer Res. Dooley M, Lamb HM. Drugs Aging. Effects of T, a new quinoline derivative, on cholinesterase activity and extracellular acetylcholine concentration in rat brain. Jpn J Pharmacol. Eur J Pharmacol. Reversible and persistent decreases in cocaine self-administration after cholinesterase inhibition: different effects of donepezil and rivastigmine. Behav Pharmacol. Donepezil treatment and the subjective effects of intravenous cocaine in dependent individuals. Individual predictors of the subjective effects of intravenous cocaine. Psychiatry Res. Arlington, Virginia: American Psychiatric Association; Text Revision ed. Importance of vacutainer selection in forensic toxicological analysis of drugs of abuse. J Anal Toxicol. Anal Chem. A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry method for quantitation of cocaine and benzoylecgonine in human plasma. Some comments on frequently used multiple endpoint adjustment methods in clinical trials. Stat Med. Pharmacokinetics of cocaine and metabolites in human oral fluid and correlation with plasma concentrations after controlled administration. Ther Drug Monit. Duysen EG, Lockridge O. Drug Metab Dispos. Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin. J Biol Chem. Inhibition of human cholinesterases by drugs used to treat Alzheimer disease. Alzheimer Dis Assoc Disord. Masuda Y. Donepezil overdose: a tenfold dosing error. Ann Pharmacother. Eur Neuropsychopharmacol. Cardiac safety of donepezil in elderly patients with Alzheimer disease. Intern Med. Modulation of the lethal effects of cocaine by cholinomimetics. Life Sci. Donepezil markedly improves long-term survival in rats with chronic heart failure after extensive myocardial infarction. Circ J. Novel strategies and underlying protective mechanisms of modulation of vagal activity in cardiovascular diseases. Br J Pharmacol. Pharmacokinetic and pharmacodynamic profile of donepezil HCl following evening administration. Br J Clin Pharmacol. Cocaine abuse versus cocaine dependence: cocaine self-administration and pharmacodynamic response in the human laboratory. Copy Download.
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In utero exposure to poisons and drugs e. The major protein interacting with these compounds is butyrylcholinesterase BuChE , which attenuates the effects of such xenobiotics by their hydrolysis or sequestration. Therefore, we studied BuChE expression during placental development. However, cytochemical staining detected primarily BuChE activity in first-trimester placenta but AChE activity in term placenta. However, BuChE in the serum of a heterozygote and a homozygous normal were similar in their drug affinities. Therefore, heterozygous serum or placenta can protect the fetus from drug or poison exposure, unlike homozygous atypical serum or placenta. Genotype analyses revealed that heterozygous carriers of atypical BuChE were threefold less frequent among 49 patients with placental malfunction than among 76 controls or the entire Israeli population. These observations exclude heterozygote carriers of atypical BuChE from being at high risk for placental malfunction under exposure to anticholinesterases. This is a preview of subscription content, log in via an institution to check access. Rent this article via DeepDyve. Institutional subscriptions. Balasubramanian, A. Have cholinesterases more than one function? Trends Neurosci. Google Scholar. Booth, A. An improved method for the preparation of human placental syncytiotrophoblast. Placenta 1: — Chasnoff, I. Cocaine use in pregnancy. Chatonnet, A. Comparison of butyrylcholinesterase and acetylcholinesterase. Time to pregnancy and occupational exposure to pesticides in fruit growers in The Netherlands. Ehrlich, G. Genomics — Gatley, S. Activities of the enantiomers of cocaine and some related compounds as substrates and inhibitors of plasma butyrylcholinesterase. Guller, S. Steroid metabolizing enzymes associated with the microvillar membrane of human placenta. Steroid Biochem. Hahn, T. Location and activities of acetylcholinesterase and butyrylcholinesterase in the rat and human placenta. Jbilo, O. Tissue distribution of human acetylcholinesterase and butyrylcholinesterase messenger RNA. Toxicon — Jeong, T. Role of metabolism by esterase and cytochrome P in cocasine-induced suppression of the antibody response. Jones, C. Ultrastructure of the normal human placenta. Electron Microsc. Karnovsky, M. A direct coloring thiocholine method for cholinesterases. Karpel, R. Expression of three alternative acetylcholinesterase messenger RNAs in human tumor cell lines of different tissue origins. Cell Res. Khalfoun, B. Characterization of the human syncytiotrophoblast plasma membrane associated components. Layer, L. Novel functions of cholinesterases in development, physiology and disease. LeDuc, B. Cocaine toxicity in cultured chicken hepatocytes: Role of cytochrome P Lev-Lehman, E. Antisense inhibition of acetylcholinesterase gene expression causes transient hematopoietic alterations in vivo. Gene Ther. Levene, C. Genetic polymorphisms among Bukharan and Georgian Jews in Israel. Liao, J. Monoclonal antibodies against brain acetylcholinesterase which recognize the subunits bearing the hydrophobic anchor. Lockridge, O. Genetic variants of serum cholinesterase influence metabolism of the muscle relaxant succinylcholine. Part 1: Genetic variant of human acetylcholinesterase. Shafferman and B. Velan, Eds. Loewenstein, Y. Molecular dissection of the cholinesterase domains responsible for carbamate toxicity. Loewenstein-Lichtenstein, Y. Nature Med. MacGregor, S. Cocaine use during pregnancy: Adverse prenatal outcome. Molecular and cellular biology of the cholinesterases. May, D. Genetic differences in drug disposition. McGuire, M. Identification of the structural mutation responsible for the dibucaine-resistant atypical variant form of human serum cholinesterase. USA — Neville, L. Anionic site interactions in human butyrylcholinesterase disrupted by two adjacent single point mutations. Intramolecular relationships in cholinesterases revealed by oocyte expression of site-directed and natural variants of human BCHE. EMBO J. Peretti, F. Cocaine fatality: An unexplained blood concentration in a fatal overdose. Prody, C. Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues. Rachmilewitz, J. H19 expression and tumorigenicity of choriocarcinoma derived cell lines. Oncogene — Rakonczay, Z. Biochemistry and pathophysiology of the molecular forms of cholinesterases. In Subcellular Biochemistry J. Harris, Ed. Rama Sastry, B. Cholinergic systems in non-nervous tissues. Ratner, D. Chronic dietary anticholinesterase poisoning. Roe, D. Metabolism of cocaine by human placentas: Implications for fetal exposure. Schwarz, M. Engineering of human cholinesterases explains and predicts diverse consequences of administration of various drugs and poisons. Successive organophosphate inhibition and oxime reactivation reveals distinct responses of recombinant human cholinesterase variants. Brain Res. Seidman, S. Cell Biol. Sherman, J. Chlorphyrifos dursban associated birth defects report of four cases. Health Int. Simone, C. Acetylcholinesterase and butyrylcholinesterase activity in the human term placenta: Implications for fetal cocaine exposure. Soreq, H. Molecular cloning and construction of the coding region for human acetylcholinesterase reveals a G, C-rich attenuating structure. Szeinberg, A. High frequency of atypical pseudocholinesterase among Iraqi and Iranian Jews. Tabacova, S. Environmental pollutants in relation to complications of pregnancy. Health Perspect. Valentino, R. Prediction of drug sensitivity in individuals with atypical serum cholinesterase based on in vitro biochemical studies. Volpe, J. Effect of cocaine use on the fetus. Whittaker, M. Cholinesterases , Karger, Basel. Whyte, A. Biochemistry of the human syncytiotrophoblast plasma membrane. In Biology of Trophoblast Y. Loke and A. Whyte, Eds. Wu, D. Choline acetyltransferase: Celebrating its fiftieth year. Zakut, H. Chorionic villi cDNA library displays expression of butyrylcholinesterase: Putative genetic disposition for ecological danger. Download references. You can also search for this author in PubMed Google Scholar. Reprints and permissions. Sternfeld, M. Cell Mol Neurobiol 17 , — Download citation. Issue Date : June Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Cellular and Molecular Neurobiology Aims and scope Submit manuscript. Abstract 1. Access this article Log in via an institution. Efflux transporters in rat placenta and developing brain: transcriptomic and functional response to paracetamol Article Open access 06 October Characterization of a human placental clearance system to regulate serotonin levels in the fetoplacental unit Article Open access 23 August Developmental expression of catecholamine system in the human placenta and rat fetoplacental unit Article Open access 23 March Google Scholar Booth, A. Google Scholar Chasnoff, I. Google Scholar Chatonnet, A. Google Scholar de-Cock, J. Google Scholar Ehrlich, G. Google Scholar Gatley, S. Google Scholar Guller, S. Google Scholar Hahn, T. Google Scholar Jbilo, O. Google Scholar Jeong, T. Google Scholar Jones, C. Google Scholar Karnovsky, M. Google Scholar Karpel, R. Google Scholar Khalfoun, B. Google Scholar Layer, L. Google Scholar LeDuc, B. Google Scholar Lev-Lehman, E. Google Scholar Levene, C. Google Scholar Liao, J. Google Scholar Lockridge, O. Google Scholar Loewenstein, Y. Google Scholar Loewenstein-Lichtenstein, Y. Google Scholar MacGregor, S. Google Scholar May, D. Google Scholar McGuire, M. Google Scholar Neville, L. Google Scholar Peretti, F. Google Scholar Prody, C. Google Scholar Rachmilewitz, J. Google Scholar Rakonczay, Z. Google Scholar Rama Sastry, B. Google Scholar Ratner, D. Google Scholar Roe, D. Google Scholar Schwarz, M. Google Scholar Seidman, S. Google Scholar Sherman, J. Google Scholar Soreq, H. Google Scholar Szeinberg, A. Google Scholar Tabacova, S. Google Scholar Valentino, R. Google Scholar Volpe, J. Google Scholar Whittaker, M. Google Scholar Whyte, A. Google Scholar Wu, D. Google Scholar Zakut, H. Google Scholar Download references. View author publications. Rights and permissions Reprints and permissions. About this article Cite this article Sternfeld, M. Copy to clipboard. Search Search by keyword or author Search. Navigation Find a journal Publish with us Track your research.
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