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Formed in 2003. Slow to mid-paced goregrind. Morocco (Israel, Belgium). Signed to Last House On The Right Records.
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Formed in 2003. Slow to mid-paced goregrind. Morocco (Israel, Belgium). Signed to Last House On The Right Records.
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Formed in 2003. Slow to mid-paced goregrind. Morocco (Israel, Belgium). Signed to Last House On The Right Records.
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Free Estimates 20+ Years of Demolition Experience Short Timelines Licensed & Insured Asbestos Certified
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Choose Triple D Demolition for your next project. As a professional demolition company, we ensure all interior, exterior, and mobile home demolitions are completed efficiently, safely, and on time. If this is your first time scheduling demolition services in Howell, MI, please take a look at our list of frequently asked questions.
Q: Are appliances considered contents? Do they cost extra in a mobile home demolition?
A: No. We do not consider appliances to be contents because they normally come with a home upon purchase. We do not charge any additional prices for appliances.
Q: Do I need to disconnect my electric, gas, or water before any mobile home demolition?
A: If you are located inside of a mobile home park, you do not need to disconnect utilities. We will work with your maintenance team to disconnect your utilities at the start of the demolition. If your mobile home is not located in a mobile home park, you do need to disconnect utilities.
Q: Do you haul away the mobile home or demolish it on-site?
A: We demolish the mobile home on-site. We use an excavator, and then place the debris in a dumpster to haul away.
Q: Do I need a permit for a residential demolition?
A: You need a permit for all demolition services for houses, garages, barns, etc. If you need demolition completed for pools, sheds, or tennis courts, please contact your local building department to verify permit requirements.
Q: Can the concrete remain during my residential demolition?
A: Absolutely, as long as the concrete pad is in good condition and your local building inspector approves it after demolition completion. In the event that approval is not granted, we will be required to remove the concrete at an extra cost.
Q: Will you pump water from my pool before my residential demolition?
A: Yes. When scheduling your demolition services, please let us know that you will need your pool pumped.
Q: What forms of payments do you take?
A: We accept cash, check, and credit cards for all demolition services. Please note that credit card transactions will result in a four percent service fee.
A: Our demolition company serves Michigan cities in Wayne County, Washtenaw County, Oakland County, Livingston County, Ingham County, and Genesee County. These include Ann Arbor, Auburn Hills, Blackman, Brighton, Chelsea, Detroit, Fenton, Flint, Fowlerville, Grand Blanc, Grand Ledge, Lansing, Leslie, Livingston County, Livonia, New Lothrop, Novi, Owosso, Pinckney, Pontiac, Saline, Sterling Heights, St Johns, Troy, Warren, and Westland. Please see the below map of our service areas. For additional information, please
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If you still have questions about your demolition project, don’t hesitate to reach out to our friendly demolition company. No matter what project you’re considering, we have the tools, expertise, and knowledge to get it done right the first time. Call Triple D Demolition today to learn more about our demolition services.
Volume 35, Issue 4 , 29 June 2004 , Pages 895-904
Author links open overlay panel Tomasz Bączek
Copyright © 2004 Elsevier B.V. All rights reserved.
The aim of the work was to explore the identification of proteins from Saccharomyces cerevisiae using combined capillary reversed-phase liquid chromatography (RPLC) and in-solution isoelectric focusing (sIEF) for fractionation of peptides prior to mass spectrometry analysis. That method was proved to be the alternative separation method for complex mixtures of protein tryptic digests in proteomics. Analysis of the identification of peptides was performed with the use of electrospray ionization-ion trap tandem mass spectrometry (ESI-IT-MS/MS). First, the sIEF fractionation was carried out prior to separation and mass spectrometry identification by nano-LC/ESI-MS/MS instrument. The proposed approach based on sIEF and nano-LC/ESI-MS/MS analysis was proved to be an efficient and accurate alternative fractionation method of complex protein digests and can be considered as the useful tool for identification of proteins. Moreover, analytical information from that approach can be considered as the additional source of database matching constraint and can be valuable tool for analytical and bioinformatics studies of peptides fractionation in proteomics. Based on the MS/MS results obtained with ESI-IT-MS/MS instrument, 851 proteins from S. cerevisiae were identified. However, after careful analysis of the data reduction in number of proteins to 126 was obtained. Those results are discussed and interpreted in the view of the evaluation method used.
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