Atroshchenko Mikhail Sperm Stallion

Atroshchenko Mikhail Sperm Stallion

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All-Russian Scientific Research Institute for Horse Breeding (ARRIH), Ryazan Oblast, Rybnovskij District 391105, Russia. Author profile
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State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency (SRC-FMBC), 46, Zhivopisnaya Str, Moscow 123098, Russia. Author profile
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State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency (SRC-FMBC), 46, Zhivopisnaya Str, Moscow 123098, Russia. Author profile
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0000-0001-6706-8606
Center for Veterinary Cell Medicine, 8/1 Tvardovsky Str, Moscow 123458, Russia. Search articles by 'Sergey B Stavitsky'
All-Russian Scientific Research Institute for Horse Breeding (ARRIH), Ryazan Oblast, Rybnovskij District 391105, Russia. Search articles by 'Alexander M Zaitsev'
All-Russian Scientific Research Institute for Horse Breeding (ARRIH), Ryazan Oblast, Rybnovskij District 391105, Russia. Search articles by 'Valery V Kalaschnikov'
Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia. Author profile
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State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency (SRC-FMBC), 46, Zhivopisnaya Str, Moscow 123098, Russia. Author profile
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1.
All-Russian Scientific Research Institute for Horse Breeding (ARRIH), Ryazan Oblast, Rybnovskij District 391105, Russia.

2.
State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency (SRC-FMBC), 46, Zhivopisnaya Str, Moscow 123098, Russia.

3.
Center for Veterinary Cell Medicine, 8/1 Tvardovsky Str, Moscow 123458, Russia.

4.
Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia.

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The main goal of our study was to determine a set of thawed stallion sperm characteristics that have predictive value for the pregnancy rate (PR) of mares after artificial insemination (AI). DNA fragmentation and survival of sperm during hypothermic storage were studied in addition to routinely determined semen characteristics such as concentration, percentage of motile spermatozoa, and morphology. To estimate DNA fragmentation, a modified hallo assay was applied. Sperm survival was determined within hours as the ability of spermatozoa to maintain progressive motility (PM) during the storage of ejaculate diluted with lactose-chelate-citrate-yolk (LCCY) medium at +4 °C. Strong positive correlation between PR and thawed sperm motility (r = 0.90, p < 0.05) as well as between PR and sperm survival (r = 084, p < 0.05) was revealed. There was also a strong negative correlation between PR and DNA damages in spermatozoa (r = -0.94, p < 0.05). We found no dependence of PR on normal morphology spermatozoa percentage in thawed semen. We concluded that the sperm activity, survival, and DNA fragmentation should be considered as the sufficient reproductive characteristics of semen to evaluate the quality of frozen/thawed sperm and prediction of PR.
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Animals : an Open Access Journal From MDPI ,
09 Dec 2019 , 9(12): E1099 DOI:
10.3390/ani9121099 PMID: 31818047 PMCID: PMC6940853

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Affiliations

1 Russian Research Institute for Farm Animal Genetics and Breeding - Branch of the L. K. Ernst Federal Science Center for Animal Husbandry, 55A, Moskovskoye Sh., Tyarlevo, Pushkin, St. Petersburg, 196625, Russia.

2 All-Russian Research Institute for Horse Breeding, Rybnovsky District, Ryazan Oblast, 391105, Russia.

3 School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.

4 L. K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy, Podolsk, Moscow Oblast, 142132, Russia.

Elena V Nikitkina et al.

Anim Biosci .

2022 .

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Affiliations

1 Russian Research Institute for Farm Animal Genetics and Breeding - Branch of the L. K. Ernst Federal Science Center for Animal Husbandry, 55A, Moskovskoye Sh., Tyarlevo, Pushkin, St. Petersburg, 196625, Russia.

2 All-Russian Research Institute for Horse Breeding, Rybnovsky District, Ryazan Oblast, 391105, Russia.

3 School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.

4 L. K. Ernst Federal Research Center for Animal Husbandry, Dubrovitsy, Podolsk, Moscow Oblast, 142132, Russia.

Gmel AI, Burger D, Neuditschko M.
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Genes (Basel). 2021 Sep 25;12(10):1501. doi: 10.3390/genes12101501.
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Greiser T, Sieme H, Martinsson G, Distl O.
Greiser T, et al.
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Anim Reprod Sci. 2019.

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Objective:

The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds.

Methods:

Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom™ Equine Genotyping Array.

Results:

We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME8, OR2AP1 and OR6C4. Potential implications of effects of these genes on sperm motility are herein discussed.

Conclusion:

The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.

Keywords:

Candidate Genes; Cryopreservation; Equus caballus; SNPs; Sperm; Stallion.

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Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
All-Russian Research Institute for Horse Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
All-Russian Research Institute for Horse Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
Russian Research Institute of Farm Animal Genetics and Breeding
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The use of cryopreserved sperm has become a routine in horse breeding. Genetic factors affecting cryoresistance remain poorly understood. The aim of the study was to search for genome-wide associations with a decreasing in sperm motility after freezing. Semen of 96 horses was frozen. Sperm motility was evaluated by computer-assisted semen analysis (CASA)( ArgusSoft, Russia) be
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