Assay comet sperm
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Comet Assay - an overview | ScienceDirect Topics
Exact tests powered by SpermComet® | Sperm DNA Analysis . . .
The use of comet assay to assess DNA integrity of boar . . .
Chapter 21 - DNA Damage: COMET Assay | Basicmedical Key
Human sperm DNA integrity assessed by the Comet and ELISA . . .
Sperm Evaluation Using the Comet Assay | SpringerLink
Fluctuation of "sperm DNA integrity" in accordance with . . .
Comet assay - Wikipedia
(PDF) The Comet Assay in Male Reproductive Toxicology
Sperm DNA Integrity Tests | Center for Male Reproductive . . .
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The comet assay is a cheap and one of the most sensitive techniques available to measure sperm DNA damage . The level of sperm DNA damage measured by the comet assay is closely associated to other direct method such as TUNEL assay (Shamsi et al ., 2008) . The comet assay can be used to measure DNA damage in a variety of cell types, including sperm .
SpermComet® is our proprietary technology powering the Exact range of sperm DNA tests . Independent research has shown that SpermComet® technology is the best performing DNA fragmentation tool in terms of sensitivity, specificity and predictive power . SpermComet® is the only DNA fragmentation assay guaranteed for testicular samples .
The comet assay, under neutral conditions, allows the assessment of DNA integrity influenced by sperm ageing, which is manifested in DNA double-strand breaks .
There are a variety of tests available to measure the level of DNA strand breaks in sperm and the most commonly used tests are the Comet assay, Terminal deoxyuridine Nick End Labelling (TUNEL) assay, Sperm Chromatin Structure Assay (SCSA), and the Sperm Chromatin Dispersion (SCD) Assay or Halo test .
The ELISA and Comet assays, two techniques that detect DNA damage in cells, are compared in this study of DNA integrity in human sperm . Both techniques rely on alkaline unwinding for the release of single strands of DNA from the nucleus .
Among available sperm chromatin quality assays, the comet assay presents the advantage of collecting data at the level of the single cell, opening the possibility of analysis to severely oligozoospermic patients .
In a study performed using the alkaline COMET assay, NT-condensed and NT-decondensed methods, Irvine et al . found a significant negative correlation between sperm DNA damage and semen quality, especially in sperm count .
A comet assay can determine the degree of DNA fragmentation in sperm cells . The degree of DNA fragmentation has been associated with outcomes of in vitro fertilization . The comet has been modified for use with sperm cells as a tool for male infertility diagnosis
A rapid, sensitive and reliable method to detect DNA damage and assess the integrity of the genome within single cells is that of the comet or single-cell gel electrophoresis assay . The present . . .
The TUNEL assay is a highly sensitive and reproducible method of assessing the integrity of sperm DNA . It measures the number of breaks or nicks in the DNA of the sperm . It can be performed on as few as 1,000 sperm, even on sperm retrieved from a small testis biopsy .
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Assay comet sperm
