Anju Karki

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Purdue University | Purdue · Department of Biological Sciences
We hypothesized that basic helix-loop-helix (bHLH) MIST1 (BHLHA15) is a "scaling factor" that universally establishes secretory morphology in cells that perform regulated secretion. Here, we show that targeted deletion of MIST1 caused dismantling of the secretory apparatus of diverse exocrine cells. Parietal cells (PCs), whose function is to pump a...
Transcriptional networks that govern secretory cell specialization, including instructing cells to develop a unique cytoarchitecture, amass extensive protein synthesis machinery, and be embodied to respond to endoplasmic reticulum (ER) stress, remain largely uncharacterized. In this study, we discovered that the secretory cell transcription factor...
Liposarcomas (LPSs) are the most common soft-tissue cancer. Because of the lack of animal models, the cellular origin and molecular regulation of LPS remain unclear. Here, we report that mice with adipocyte-specific activation of Notch signaling (Ad/N1ICD) develop LPS with complete penetrance. Lineage tracing confirms the adipocyte origin of Ad/N1I...
Gemcitabine is the standard-of-care for chemotherapy in patients with pancreatic adenocarcinoma and it can directly incorporate into DNA or inhibit ribonucleotide reductase to prevent DNA replication and, thus, tumor cell growth. Most pancreatic tumors, however, develop resistance to gemcitabine. Polo-like kinase 1 (Plk1), a critical regulator in m...
Acinar cells of the exocrine pancreas are tasked with synthesizing, packaging and secreting vast quantities of pro-digestive enzymes to maintain proper metabolic homeostasis for the organism. Because the synthesis of high levels of hydrolases is potentially dangerous, the pancreas is prone to acute pancreatitis (AP), a disease that targets acinar c...
Schematic of how the Mist1lox/+ mice were generated through homologous recombination.
LoxP sites flank the entire Mist1 coding region which is contained within exon 2.
(TIF)
H&E images of whole sections from post-AP iMist1myc pancreata.
(A) Mist1CreERT/+ (left) and iMist1myc (right) pancreata 7d post-AP. iMist1myc pancreata contain very few Amylase+ acini structures at this time point. Inset shows a higher magnification of the boxed area. (B) Mist1CreERT/+ (left) and iMist1myc (right) pancreata 8w post-AP. At this time...
Quantification of AP damage in Mist1CreERT/+ animals.
(A) Morphometric analysis of Mist1CreERT/+ pancreata over the indicated times. (B) Relative CLUSTERIN, K19 and AMYLASE protein levels in IF sections from Mist1CreERT/+ pancreata at the indicated times and normalized to control values. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s.—not significant.
(T...
Schematic of the LSL-Mist1myc transgene in iMist1myc mice.
Mist1CreERT/+/iMist1myc mice express the iMist1myc transgene exclusively in acinar cells upon Tam induction.
(TIF)
Quantification of AP damage in iMist1 animals 6h-4d post-AP.
(A) Morphometric analysis of iMist1 pancreata over the indicated times. (B) Relative E-CADHERIN and CLUSTERIN protein levels in IF sections from iMist1 pancreata at the indicated times and normalized to control values. (C) Relative CD45, SMA and VIMENTIN protein levels in IF sections from...
Quantification of AP damage in Mist1CreERT/lox (Mist1 cKO) animals.
(A) Morphometric analysis of Mist1 cKO pancreata over the indicated times. (B) Relative CLUSTERIN, K19 and AMYLASE protein levels in IF sections from Mist1 cKO pancreata at the indicated times and normalized to control values. **p ≤ 0.01; ***p ≤ 0.001; n.s.—not significant.
(TIF)
Quantification of AP damage in iMist1 animals 7d-8w post-AP.
(A) Morphometric analysis of iMist1 pancreata over the indicated extended times. (B) Relative CLUSTERIN, K19 and AMYLASE protein levels in IF sections from iMist1 pancreata at the indicated times and normalized to control values. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s.—not significant....
IF images of Mist1CreERT/+ pancreata revealing nuclear MIST1 protein exclusively in acinar cells.
Islets and ducts remain MIST1 negative.
(TIF)
The Wnt/β-catenin signaling pathway has been identified as one of the predominantly upregulated pathways in castration-resistant prostate cancer (CRPC). However, whether targeting the β-catenin pathway will prove effective as a CRPC treatment remains unknown. Polo-like kinase 1 (Plk1) is a critical regulator in many cell cycle events and is signifi...
I have quite a number of western blots to be quantified. I am also looking for alternative techniques to quantify protein bands. 
I want to stain tissue sections for PTF1a. Is anybody using a good antibody that I can use?
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