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Official websites use. Share sensitive information only on official, secure websites. MacDonald J. E-mail: mac. Opioid agonists are the most effective treatment for pain, but their use is limited by side effects, tolerance and fears of addiction and dependence. A major goal of opioid research is to develop agonists that have high analgesic efficacy and a low profile for side effects, tolerance, addiction and dependence. Unfortunately, there is a serious lack of experimental data comparing the degree to which different opioids produce these effects in humans. In contrast, a wide range of experimental techniques from heterologous expression systems to behaviour assessment in whole animals have been developed to study these problems. The objective of this review is to describe and evaluate these techniques as they are used to study opioid efficacy, tolerance, addiction and dependence. This article is part of a themed issue on Translational Neuropharmacology. Keywords: analgesia, opioid, morphine, opioid signalling, withdrawal, opioid efficacy, mu-receptor. Opioids are the most effective treatment for pain. Unfortunately, opioid use is limited by serious adverse effects such as respiratory depression, sedation and constipation. The development of tolerance, dependence and addiction during chronic opioid use further limit the clinical utility of these drugs. Opioid tolerance is characterized by a reduced responsiveness to an opioid agonist such as morphine and is usually manifest by the need to use increasing doses to achieve the desired effect. Clinically, more than fold dose escalations of opioid dose in chronic pain management are common Buntin-Mushock et al. Addiction as defined by the compulsive, harmful use criteria of the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition is common among recreational opioid users, but relatively rare in chronic pain patients Cowan et al. The terms dependence and addiction are commonly used interchangeably, but the former will be used here in the context of the withdrawal syndrome that is characteristically observed on cessation of chronic opioid use or administration of opioid antagonists and the latter to models of compulsive drug use in animals and humans. Although animal studies have reported differences in analgesic efficacy, tolerance, addiction or withdrawal for different opioids, surprisingly few human studies have examined these differences systematically. Although knowledge of genetic markers associated with opioid addiction and treatment are beginning to emerge Yuferov et al. For more than a century, efforts to overcome the adverse effects of opioids have met with limited success. All opioids that produce analgesia also can cause tolerance, addiction and withdrawal, and all available opioids are misused Hernandez and Nelson, Successful approaches to pharmacotherapy of opioid addiction continue to rely largely on substitution of short-acting agonists heroin often used dangerously usually injection with oral administration of long-acting high-efficacy agonists methadone or partial agonists buprenorphine Lobmaier et al. Nonetheless, a variety of animal and in vitro models provide a solid framework for translational research contributing to developments in opioid therapeutics that may reduce the severity of one or more of these adverse effects. Long-acting opioid agonists have improved the management of ongoing pain and new formulations have improved the management of breakthrough pain Inturrisi, , but attempts to exploit different opioid receptor types have been less successful. Recent promising preclinical approaches to limit tolerance, dependence and addiction include simultaneous activation of more than one opioid receptor type e. Efforts to better understand analgesic efficacy, tolerance, dependence and addiction as a way to enhance the analgesic effects and limit the liabilities have used a range of methodologies. The approaches include analyses at both the behavioural and cellular level in whole animals in addition to the use of reduced preparations such as heterologous cell systems and in vitro slice recordings. The type of information provided and the ability to generalize to clinical situations varies with the technique and issue. The present review will examine both behavioural and cellular approaches applied to four issues that limit opioid use in the treatment of clinical pain: limited antinociceptive efficacy, tolerance, addiction, and dependence. Opioid antinociception, tolerance, dependence and addiction have been examined using a wide range of techniques in whole animals. The main advantage of using whole animals is that the nervous system is intact and opioid effects are linked to behaviour. A major problem, particularly with investigation of new drug candidates is that many studies fail to account for differences in pharmacokinetics and properties such as receptor efficacy. A range of different treatment schedules are commonly used to induce tolerance, dependence and addiction including repeated injections, osmotic minipumps, implantation of morphine base pellets standardized morphine pellets can be obtained from the National Institute on Drug Abuse, USA or sustained release emulsions. In addition, the physiological relevance of the species and behavioural assessment tools are quite variable and can be questioned. Pain sensitivity and antinociception have been shown to vary with mouse strain Mogil et al. Nonetheless, years of research have provided a range of methodologies to assess antinociceptive efficacy, tolerance, addiction and dependence. A vast amount of information about the cellular basis for opioid efficacy, tolerance, addiction and withdrawal has accumulated since the identification of genes encoding opioid receptors in the early s Chen et al. Some of these data are derived by correlating cellular and behavioural changes produced by opioid administration in whole animals. This is a powerful approach in that it links cellular changes to specific behaviours such as antinociception or tolerance. These studies typically examine changes in the amount of a protein, but physiological changes can be studied in tissue taken from animals treated previously with opioids. Interpretation of the significance of these changes can be difficult. Living tissue also can be removed from the nervous system to study neural responses in vitro. This approach provides a great deal of control of drug concentrations and a well-defined physiological end-point with the added benefit of having most connections to other cells intact. The most obvious deficits are the loss of afferent and efferent connections to the structure being studied and that mechanisms in one cell type may not be the same as another. In addition, the control that comes from isolating specific channels in specific neurons also prevents analysis of the normal function of the system. Moreover, the physiological relevance of opioid induced changes cannot be known. Opioid activation of Kv channels in periaqueductal gray neurons almost surely contribute to the antinociceptive effects of opioids, but the relative importance of these channels over G-protein coupled inward rectifying potassium GIRK or Ca channels or modulation of glutaminergic inputs cannot be determined using in vitro techniques alone. Nonetheless, in vitro slice recordings have provided a depth of understanding of opioid signalling pathways that would not have been possible with other approaches. The effects of withdrawal of opioid drugs on cellular properties of tissues taken from animals after chronic treatment with opioids also have been studied Williams et al. Excessive increases in action potential or synaptic activity during spontaneous or antagonist precipitated withdrawal in neurons and systems usually inhibited by opioid agonists appear to reflect dependence at the cellular level but are not necessarily causally connected with dependence in the behaving animal or human. Naturally occurring neurons can be dissociated and maintained in culture. This is a common approach to study the effects of opioids on neurons from the dorsal root ganglion Werz and Macdonald, , but can also be used with neurons in the brain and spinal cord. The advantage of this approach is the control that comes with an isolated preparation and the fact that these are naturally occurring neurons with all of the signalling proteins that normally exist. The problem is that removal of the neurons damages the cells and deprives them of the connections, growth factors and other chemicals in their natural environment. For example, microglial activation appears to contribute to opioid tolerance Zhou et al. The ability to maintain healthy cells in culture and express proteins in those cells has greatly enhanced the analysis of opioid mechanisms of action. The main advantage of this technique is control. The function of specific proteins can be studied by inserting a protein or the genetic material necessary to make the protein into a cell with known characteristics. This approach is particularly useful to determine the electrophysiological or biochemical properties of a protein Tate and Grisshammer, The amount of protein and the presence or absence of various signalling pathways will influence opioid effects. Although over-expression enhances the ability to examine protein biochemistry, protein behaviour and stoichiometry of other signalling proteins can change depending on the level of receptor expression Law et al. Efficacy, as the term is used here, refers to receptor signalling efficacy, or the magnitude of a receptor-mediated effect produced by a drug relative to receptor occupancy Clarke and Bond, ; Strange, Relative efficacies of a series of agonists can be estimated readily if they behave as partial agonists maximum response is less than that achieved with a full agonist which should be confirmed by demonstrating partial antagonism of a full agonist by the drug in question. A goal of opioid research is to develop compounds with high analgesic efficacy because these are less affected by tolerance development in animal studies than low-efficacy agonists see below. However, low-efficacy agonists can have other advantages, for example, the low-efficacy opioid, buprenorphine, produces less respiratory depression and is therefore safer in humans than high-efficacy agonists Dahan et al. Opioid efficacy in vivo can be measured using a wide range of techniques and endpoints in addition to analgesia. This section will review the different approaches used to evaluate opioid efficacy and consider some of the technical limitations of different approaches. With the exception of long-established evidence that buprenorphine is a low-efficacy opioid Jasinski et al. Part of the problem is that large-scale clinical trials to assess relative efficacy are difficult to conduct from both a practical and ethical standpoint. In addition, analgesic efficacy can vary with the type of pain cancer, neuropathic, surgical , method of opioid administration oral, intravenous, transdermal , and age and sex of the patient. The six most common clinically used opioids are morphine, oxycodone, hydromorphone, fentanyl, buprenorphine and methadone Pergolizzi et al. The widespread use of these opioids indicate that they are effective in treating pain. Experimental data indicate that these drugs are effective in treating pain even for chronic conditions such as neuropathic pain Eisenberg et al. However, with few exceptions the relative analgesic efficacy of opioids is not yet known in humans and the implications of this for tolerance, addiction and dependence are not well established. In contrast, the antinociceptive efficacy of opioids has been well studied in experimental animals and it is reasonable to expect that these findings might extrapolate to humans. A wide range of nociceptive tests has been developed to assess nociception in laboratory animals Le Bars et al. Rats and mice are by far the most common species used in studies assessing the antinociceptive effects of opioids. Although there are a number of studies that analyse antinociceptive efficacy specifically, it is not known if relative efficacy is consistent across nociceptive tests. Given that the antinociceptive potency of morphine varies with nociceptive test Morgan et al. A more difficult question to answer is whether the relative efficacy of different opioids varies depending on the nociceptive test. Efficacy is also difficult to assess because most nociceptive tests impose an artificial definition of antinociception to limit potential damage to the animal. For example, the two most common nociceptive tests, the tail flick and hot plate tests, typically define antinociception as a latency of 10 and 50 s respectively. The most complete analysis of antinociceptive efficacy to systemically administered opioids has been conducted by Yoburn and colleagues using partial irreversible antagonism to estimate relative efficacy tau. Other studies with rats show similar results: greater antinociceptive efficacy for fentanyl, etorphine and methadone than morphine Adams et al. Additional studies examining relative antinociceptive efficacy using chronic pain tests would help link these findings to humans where opioids are primarily used to treat chronic conditions. If these animal data are comparable to human, then the six most commonly used opioids to treat pain in humans include both high-efficacy fentanyl and relatively low-efficacy oxycodone agonists. This range of opioid efficacies indicates that factors other than efficacy are more important in determining opioid use in humans than relative efficacy. These factors include side effect profile, onset of action, drug interactions, abuse potential, cost and type of pain Pergolizzi et al. The importance of these factors is not surprising given that the overall analgesic efficacy of opioids is quite good compared with most other treatments. Of course, it is possible that the antinociceptive efficacy of opioids in humans and rodents differ. The fact that nociception is assessed differently in rodents and pain patients may contribute to a difference in measured efficacy. For example, antinociceptive efficacy on a nociceptive reflex i. Supraspinally organized nociceptive tests such as the hot plate are influenced by a range of non-nociceptive stimuli such as body weight and habituation Gunn et al. These factors have less of an influence on reflexes suggesting that nociceptive reflexes may be a good way to isolate the antinociceptive effects of opioids. However, opioid efficacy appears to vary depending on whether A-delta or C-fibres are activated by the stimulus McCormack et al. Nonetheless, opioids that are effective in the clinic, such as morphine and fentanyl, produce very good antinociceptive effects in rats tested with the hot plate or tail withdrawal Morgan et al. Other factors such as sex and chronic pain also alter the antinociceptive effects of opioids. Changes in opioid antinociception caused by chronic pain are complicated by the fact that baseline pain sensitivity is enhanced Przewlocki and Przewlocka, Less clear is whether sex and chronic pain alter the relative efficacy of opioids in humans. Comparison of opioid efficacy using other endpoints and tissue preparations has been reported. Although different opioids are used in different experiments, efficacy for these endpoints is relatively consistent with antinociceptive efficacy as the examples in Table 3 demonstrate. The main exception is that morphine efficacy can vary widely depending on the signalling pathway. For example, morphine produces good inhibition of adenylyl cyclase Keith et al. The behavioural significance of some of these signalling cascades is uncertain because opioids activate a wide range of signalling pathways, but only a subset of these are known to contribute to the antinociceptive effects Mitrovic et al. The operational definition of tolerance is a decrease in effect following repeated or prolonged administration of a specific dose. Experimentally, tolerance is best demonstrated by a rightward shift in the agonist dose-response curve after repeated administration for days or weeks. This decrease in effect can be caused by pharmacokinetic, pharmacodynamic or conditioning mechanisms. Studies claiming to demonstrate that a novel opioid produces less tolerance than conventional opioid analgesics should establish that pharmacokinetic considerations are not relevant. Few studies measure concentrations of the drug at the relevant target CNS or control for duration of action of each dose. CNS concentration is less of a concern in animal studies in which direct opioid administration into specific parts of the CNS such as the periaqueductal gray Morgan et al. Although repeated opioid administration produces some differences in neural adaptations from continuous administration Christie, , unambiguous comparison of tolerance development with different agonists requires steady state treatment e. This is rarely achieved. Despite these limitations, the measurement of tolerance is strongly influenced by opioid efficacy with high-efficacy agonists displaying less obvious tolerance than low-efficacy agonists. This relationship is well established in both behaving animals Stevens and Yaksh, and isolated cells Christie et al. Receptor signalling efficacy of both the treating and challenge opioids also influences the magnitude of tolerance, as well as efficacy for different downstream signalling cascades Berger and Whistler, Continuous administration of low- compared with high-efficacy agonists produce greater tolerance to challenge agonists regardless of the efficacy of the latter Madia et al. A major goal is to determine the mechanisms underlying tolerance so treatments that block these mechanisms can be developed to maintain analgesia for long periods of time. The problem with these studies is that numerous mechanisms for tolerance have been proposed Christie, , but no integrated theory exists. Additional research linking molecular changes to behaviour is needed to clarify the mechanism for tolerance to opioids. Dose escalation in human pain patients receiving chronic opioid therapy is well established Collett, ; Tobias, , although many patients maintain a relatively consistent dose for months once a therapeutic dose is achieved Cowan et al. Dose escalation is a common occurrence for illicit opioid users Cowan et al. The magnitude of tolerance is more difficult to ascertain in chronic pain patients. Many patients stop taking opioids because of adverse effects or ineffective pain relief Noble et al. Tolerance to the analgesic effects likely contributes to this decrease. A decrease in analgesia can also be caused by factors unrelated to tolerance such as an increase in pain related to disease progression Portenoy, Tolerance also appears to be limited when opioids are self-administered Hill et al. Thus, although tolerance to the analgesic effects of opioids has been demonstrated in pain patients, the magnitude of the problem is debatable Collett, ; Tobias, Although whole animal and cellular studies would predict that low-efficacy agonists should show greater tolerance than high-efficacy opioids see below , there is no evidence for this in the limited studies that have been performed. When directly comparing tolerance development during continuous administration of transdermal fentanyl high-efficacy versus buprenorphine low-efficacy Sittl et al. Tolerance to the antinociceptive effects of opioids in animal experiments is well documented. Tolerance has been shown with as few as a single injection Cochin and Kornetsky, ; Melief et al. Neither the mechanism underlying tolerance nor the degree to which tolerance develops to different opioids at each of these sites is necessarily the same. Indeed, at the cellular level, recent findings indicate that the mechanism underlying tolerance may differ depending on the opioid. Tolerance appears to develop to all opioids, but the magnitude of tolerance varies depending on the route of administration and agonist efficacy. Repeated injections of morphine, fentanyl, etorphine, oxycodone and meperidine in mice produce comparable rightward shifts in the dose—response curve of the same agonist for antinociception, but continuous administration of these drugs produces much greater cross-tolerance to morphine than etorphine or fentanyl Duttaroy and Yoburn, Continuous intrathecal infusion of morphine also produces greater tolerance than infusion of DAMGO or fentanyl Stevens and Yaksh, indicating, as expected, that high-efficacy agonists show less tolerance than lower-efficacy agonists. In fact, rats show a greater shift in the morphine than the sufentanyl dose—response curve even when pretreated with sufentanyl to induce tolerance Sosnowski and Yaksh, The opposite also appears to be true. However, this relationship between efficacy and cross-tolerance to morphine is only evident with continuous, not repeated administration of the pretreatment opioid Madia et al. These issues can only be properly resolved by directly determining receptor tolerance produced by different agonists in tissue from treated animals. Tolerance can be studied at the cellular level by either correlating chronic opioid administration with changes in neural signalling or by measuring changes in cell activity with prolonged application of an opioid. Analysis of data for all downstream mechanisms is too large an undertaking for this review especially given that few of these studies have compared the effects of different opioids. This rapid loss of receptor sensitivity occurs much more quickly several minutes than the development of tolerance to the antinociceptive effects of opioids days to weeks , so the relevance to tolerance remains unclear see above. When both signalling efficacy and this acute desensitization have been directly measured in the same heterologous expression systems Borgland, , the ability to produce desensitization was directly correlated with receptor-signalling efficacy, albeit rightward shifted. For example, morphine produces rapid tolerance, but relatively little desensitization to inhibition of GIRK currents in locus coeruleus neurons Dang and Williams, These data suggest that acute desensitization may not be a good predictor of tolerance to the antinociceptive effects of opioids. Of course, this conclusion may depend on the signalling pathway involved. Several studies indicate that different mechanisms underlie shot-term tolerance to high- and low-efficacy agonists Hull et al. Thus, a causal relationship between acute desensitization and tolerance may yet be established. One possibility is that desensitization contributes to tolerance to high-, but not low-efficacy agonists. The experimental data are clear about one thing; tolerance to opioids is rapid and pronounced. As described above, this is evident at both the behavioural and cellular level. Although the long-term use of opioids to treat chronic pain suggests that pain may interfere with the development of tolerance to opioids in humans Cowan et al. Both of these findings have important clinical implications and need to be examined in human studies. Drug addiction is defined as an uncontrolled craving for a substance and is manifested in drug-seeking behaviours. Opioid addiction, heroin addiction in particular, has been well characterized scientifically Rosenberg, and portrayed to the public in graphic detail in movies such as Trainspotting and Requiem for a Dream. The power of opioids to motivate behaviour is clearly evident in both cases. The one exception is the relatively low incidence of addiction to opioids in chronic pain patients Fishbain et al. The lack of addiction in this case could be caused by pain interfering with the rewarding properties of opioids, limited liability as a result of the type of opioid and route of administration used to treat pain e. Animal studies are well suited to test these hypotheses. Given that addiction is defined by craving, experimental models are limited to behavioural approaches in intact animals. A number of molecular mechanisms that contribute to drug addiction have been described and are reviewed elsewhere Nestler, Although these animal models of addiction have limitations, they have good predictive validity for abuse liability in humans. Self-administration is the best and most commonly used model of opioid addiction in animals. These studies typically require an animal to press a lever to receive a drug. The drug is usually delivered through an intravenous catheter, although other routes of administration such as oral or intracranial can be used. Most self-administration studies use rats, but the procedure is similar whether the experimental subjects are transgenic mice or humans. The most striking finding is that animals will reliably press a lever to self-administer the same drugs abused by humans Johanson and Balster, ; Balster, ; Brady, Although there are a number of variations to this approach such as different reinforcement schedules, the use of second ordering conditioning and direct intracranial drug administration Richardson and Roberts, ; McBride et al. The validity of self-administration studies in animals as a way to assess the reinforcing properties of drugs has been validated in self-administration studies in humans Haney, The procedure for self-administration of opioids in humans is similar to that in animals except that human administration can be via intravenous or intranasal routes, human volunteers are typically abusing opioids prior to the start of the study, and subjects are given a choice between repeat administration of an opioid and cash as a way to assess the magnitude of reinforcement Haney and Spealman, ; Comer et al. Almost all opioids produce an increase in responding using these procedures, although tolerance appears to limit the reinforcing properties of some opioids, such as buprenorphine, in subjects maintained on opioids Winger and Woods, ; Comer et al. The high subjective rating for buprenorphine is probably related to its ability to limit opioid withdrawal because it was not self-administered at levels greater than placebo. Similar potency ratings for opioid self-administration have been reported in rhesus monkeys Winger and Woods, Opioid self-administration has proven useful in testing treatments for opioid addiction. The three currently approved drug treatments for addiction, methadone, buprenorphine and naltrexone, have similar effects in human addicts and heroin self-administration studies Haney and Spealman, Thus, self-administration studies in animals are a useful tool to screen potential treatments for opioid addiction. Conditioned place preference CPP also has been used to assess the abuse potential of drugs. The conditioning part involves pairing drug administration with a specific environment and the drug vehicle with a different environment. The rewarding properties of the drug are assessed by allowing the rat to move between the two environments in the absence of drug. An increase in the amount of time spent in the drug-paired environment indicates that the previously administered drug has rewarding properties. A preference has been shown to occur following a single pairing with morphine Bardo and Neisewander, , although the magnitude of the preference increases with the number of conditioning trials Lett, The primary problem with CPP is that the relationship to drug abuse is not clear. A preference indicates that a drug is rewarding, but it is not known whether environments associated with drug administration in humans are preferred as would be predicted by CPP studies in animals. Nonetheless, most drugs that are self-administered by animals also produce CPP Bardo and Bevins, This correspondence between self-administration and CPP appears to be particularly good for opioids. That is, opioids appear to be rewarding whether self-administered or passively injected during place conditioning. The main advantage of CPP over the self-administration procedure is that preference is assessed in the absence of drug because no drug is administered on the test day. Thus, locomotor and other unconditioned effects produced by opioids do not confound CPP. Animal models also have been developed to study drug relapse. Both self-administration and CPP procedures have been used to study reinstatement of drug-seeking behaviour Lu et al. Reinstatement requires an initial training phase in which the drug is paired with lever pressing or a specific environment, followed by extinction trials in which drug is not administered. Reinstatement occurs when the animal is re-exposed to the drug, cues associated with the drug, other drugs or environmental stressors Shalev et al. Similar stimuli produce relapse in humans Carter and Tiffany, ; Sinha, Most of what is known about relapse comes from studies using heroin and cocaine Shalev et al. The studies described above indicate that animal studies of addiction and relapse are a good model for these behaviours in humans. Although the term dependence may be used more or less interchangeably with addiction, it can be defined as the presence of withdrawal signs upon removal of the drug, as it is here. In humans, the withdrawal syndrome consists of signs and symptoms including stomach cramps, diarrhoea, rhinorrhoea, sweating, elevated heart rate and increased blood pressure, irritability, dysphoria, hyperalgesia and insomnia Cushman and Dole, After abrupt cessation of heroin or morphine use, the withdrawal syndrome develops over a period of less than 1 day and generally persists with declining severity for 1 week to 10 days. However, dysphoria and anhedonia can persist for much longer. The withdrawal syndrome contributes to opioid addiction during cycles of opioid use or abuse presumably because repeated dosing is maintained or escalated to avoid the withdrawal syndrome leading to development of more profound tolerance and dependence Koob and Le Moal, Since the identification of cellular adaptive processes that could mediate opioid withdrawal e. However, the extent to which the adaptations that produce withdrawal contribute to opioid addiction is uncertain because relapse is common long after the withdrawal syndrome abates and distinct neural systems appear to be involved in addiction and dependence Koob and Le Moal, ; Christie, Animal models of opioid withdrawal have been utilized in the hope of finding opioids that might induce less withdrawal and perhaps have lower addiction liability Berger and Whistler, Animal models have very strong predictive validity that the same opioids will produce dependence in humans. As has been proposed for tolerance development, both agonist receptor efficacy and endocytosis efficacy have been implicated in the propensity for dependence to develop Berger and Whistler, More importantly, it is not known if opioid efficacy has any impact on dependence in humans. All potent opioid agonists produce dependence in humans, regardless of their efficacy for receptor signalling or internalization, but whether or not severity of dependence for equi-effective opioid doses and duration of action of these differs is not known in humans or animals. Animal models also have been used to examine a range of adjuncts that can lessen the severity of withdrawal with the goal of facilitating opioid detoxification. The signs of opioid withdrawal in rodents include those referred to as somatic or vegetative signs, as well as aversive signs. The distinction is somewhat artificial although some signs are clearly mediated predominantly by adaptations in peripheral nerves while others are centrally mediated Koob et al. A number of factors should be kept in mind when studying withdrawal. Spontaneous or naloxone-precipitated withdrawal can be difficult to observe after chronic administration of very long-acting, high-affinity opioids such as buprenorphine despite development of considerable tolerance because displacement of the receptor bound agonist by naloxone is difficult to achieve Dum et al. The testing environment also affects the expression of some withdrawal signs. Jumping presumably escape attempts is expressed frequently during withdrawal in both rats and mice in environments such as large vertical observation cylinders but not in small cages. Burrowing presumably an escape behaviour is most clearly observed where the opportunity to burrow is provided by the presence of abundant bedding in the observation chamber. Some signs such as ptosis and wet-shakes are more readily scored in rats than mice. Experimental models of the aversive nature of opioid withdrawal generally involve tests of conditioned place aversion Koob and Le Moal, This approach appears to be more sensitive to low doses of antagonists than expressed signs, but the basis for this difference is not understood. Although most signs considered characteristic of opioid withdrawal are not expressed in the continued presence of opioid agonists, some signs reflect adaptive mechanisms that persist in the continued presence of opioid agonists but also are exacerbated during abstinence. For example, opioid hyperalgesia can develop after chronic opioid use in both animals and humans and appears to be due to adaptations produced by chronic agonist exposure in the medulla that control descending pain modulatory mechanisms Fishbain et al. This can be difficult when chronic treatment involves use of high doses of very low-efficacy agonists e. Although preclinical measures of opioid antinociception, tolerance, addiction and dependence need to be interpreted with caution, overall they appear to have good predictive validity for human responses to opioids. The primary problem in making this connection is the lack of randomized controlled human studies. Even the best human studies do not compare differences in analgesic efficacy or tolerance liability for different opioids. In contrast, animal studies reveal clear differences in antinociceptive efficacy and liability for tolerance depending on the opioid. Moreover, the opioids producing these different effects have been linked to different signalling cascades. The future promise of these cellular studies is the identification of specific signalling pathways for antinociception, tolerance, addiction and dependence. This knowledge will allow new compounds to be screened that stimulate the signalling pathways involved in antinociception, but not those contributing to tolerance, addiction or dependence. What is less certain is how well preclinical findings for opioids that differentially signal to different intracellular cascades, preferentially act on more than one receptor type, or act on heteromultimers will translate to humans. Animal models of tolerance are most useful when combined with parallel analyses of opioid sensitive tissues and neurons from tolerant animals as well as other cellular models that can be probed for receptor function and signalling. Cellular models are limited when used alone because the relationship of specific neurons or signalling mechanisms to analgesia, tolerance, addiction and withdrawal is difficult to know. However, the relative efficacy of various opioids is consistent across many experimental approaches and signalling pathways suggesting that these techniques have good clinical relevance. As a library, NLM provides access to scientific literature. Br J Pharmacol. Find articles by Michael M Morgan. Find articles by MacDonald J Christie. Rat tail flick studies: Walker et al. Open in a new tab. Similar articles. Add to Collections. Create a new collection. Add to an existing collection. Choose a collection Unable to load your collection due to an error Please try again. Add Cancel. Walker et al. Adams et al. Goode and Raffa Pawar et al. Sirohi et al. Kumar et al. Madia et al. Celver et al. Keith et al. Whistler et al. Koch et al. Zaki et al. Melief et al. Borgland et al. Arttamangkul et al. Trafton et al. McPherson et al. Traynor and Nahorski Saidak et al. Yu et al. Macey et al. Alvarez et al. Kovoor et al.
Analysis of opioid efficacy, tolerance, addiction and dependence from cell culture to human
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Analysis of opioid efficacy, tolerance, addiction and dependence from cell culture to human
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