Add to Wish Lists UPGRADE TO PREMIER AT NO EXTRA COST ( Premier bonus applied at checkout) You have added your selected item(s) to your cart. What do you want to do next? Recent research has shown that vitamin D is important to every cell and tissue throughout the body. From proper immune function and bone density to heart health and mood disorders, vitamin D is critical for optimal health. You should know your vitamin D blood level. Life Extension® offers a reliable vitamin D blood test at a fraction of what most commercial blood labs charge. Optimal blood levels of vitamin D are often far greater than the standard reference range. The only way to achieve an optimal level is through proper supplementation. Fasting is not required for this test. Take all medications as prescribed. The laboratory services are for informational purposes only. It is not the intention of National Diagnostics, Inc and Life Extension to provide specific medical advice but rather to provide users with information to better understand their health.
Specific medical advice including diagnosis and treatment will not be provided. Always seek the advice of a trained health professional for medical advice, diagnosis or treatment.Both the physician and the testing laboratory are independent contractors with whom National Diagnostics, Inc makes arrangements for your blood tests. Neither National Diagnostics, Inc or Life Extension will be liable for any acts or omissions of the physician, the testing laboratory, or their agents or employees. Product reviews are provided for informational purposes only and reflect solely the views and opinions expressed by the contributors and not those of Life Extension. We do not verify or endorse any claims made in these reviews. Please read the full product reviews disclaimer. The information provided on this site is for informational purposes only and is not intended as a substitute for advice from your physician or other health care professional or any information contained on or in any product label or packaging.
You should not use the information on this site for diagnosis or treatment of any health problem or for prescription of any medication or other treatment. You should consult with a healthcare professional before starting any diet, exercise or supplementation program, before taking any medication, or if you have or suspect you might have a health problem. You should not stop taking any medication without first consulting your physician.Vitamin D US > Free 25OH Vitamin D > Free 25OH Vitamin D, ELISA, 96 tests (RUO) This assay is the result of a collaboration between DIAsource ImmunoAssays S.A. and Future Diagnostics B.V. Future Diagnostics has filed a patent application assay and has granted to DIAsource ImmunoAassays the exclusive commercialization of this assay. The Free 25OH Vitamin D ELISA is a competitive ELISA assay, based on patented monoclonal antibodies, that allows for the direct measurement of the free fraction of 25OH Vitamin D. The assay is calibrated against a symmetric dialysis method.
It shows excellent sensitivity, precision and performance characteristics, as performed according to the CLSI guidelines. The assay is unique and exclusively available at DIAsource Immunoassays. The free 25(OH)Vitamin D ELISA is based on a two-step immunoassay procedure performed in a microtiterplate. During the first incubation step free 25(OH)Vitamin D [25(OH)Vitamin D2 and D3] is bound to the anti-Vitamin D antibody coated on the wall of the microtiterplate. The in vivo equilibrium between free and bound 25(OH)Vitamin D is minimally disturbed. After washing, a fixed amount of biotinylated 25(OH)Vitamin D is added to each well. The non-bound biotinylated 25(OH)Vitamin D is removed by washing and a streptavidin peroxidase conjugate is added. In a next step TMB chromogenic substrate is added. Finally the reaction is stopped by addition of Stop reagent and the absorbance [A450nm] is measured using a plate spectrophotometer. The concentration of free Vitamin D in the sample is inversely proportional to the absorbance in each sample well.
Download our Technical Review on Validation of plasma samples for the DIAsource Free 25OH Vitamin D ELISA assay. 1h30 min/30 min/ 20 min at 37°C with shaking For Research Use Only Dyamed Biotech Pte Ltd Download "Instructions for Use" Download "Material Safety Data Sheet" Download brochure "Free 25OH Vitamin D ELISA Assay" Choose my countryDIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.Please select your country to see the most accurate content available in your area.Over many centuries of human history, the shift from a hunter-gatherer to an agricultural-based to an industrialized society also meant less vitamin D from sun exposure. all developed in areas of the world that were subtropical in climate and that made living outdoors year-round a possibility. times, human beings have always spent a great deal of time outdoors.
as the human race migrated from subtropical to less temperate climates, this has changed dramatically. Whenever tests are done to measure vitamin D levels in numerous subjects, the results show that the population as a whole is suffering from chronic vitamin D deficiency. A typical study, done in the Massachusetts General Hospital, found over 50% of inpatients had vitaminThese numbers are probably reflective of most of theToday, many of us work indoors and use our cars to get from place to place, instead of walking. In addition to that, many people use sunscreen whenever they spend any time out in the sun. There’s no question that chronic, excessive exposure to sunlight increases risk of non-melanoma skin cancer. But there’s very little evidence that sensible, moderate sun exposure increases your risk of the most deadly form of skin cancer, melanoma. In fact, there’s good evidence to suggest that it may actually decrease your risk.
Unfortunately, the ongoing campaignwarning us to avoid sun exposure has lead to a widespread deficiency of vitamin D. The best place to get vitamin D is from your skin being exposed to the UV-B rays that are in normal sunlight. Vitamin D from sunlight acts as a pro-hormone, which converts into 25-hydroxyvitamin D, or vitamin D3 in UV light is divided into three bands, or wavelength ranges, which are referred to as UVA, UVB and UVC. UVB is the primary cause of sunburn caused by overexposure to sunlight. However, UVB sunlight also produces vitamin D in your skin. The amount produced depends on exposure time, tome of day, geographic location, season, clouds, pollution, a person's age, the amount of skin surface exposed, and skin color. misconception is that occasional exposure of your face and hands to sunlight is sufficient for vitamin D production. can provide 200-400 IU vitamin D during the summer months, but this is insufficient for optimal health.
You can tell you’ve reached your optimal exposure for the day when your skin turns a very light shade of pink, within 20 minutes forDark complected people may need several times the amount for the same benefits. But after that, you’re only increasing your chances of getting sunburn, and sunburn has been clearly related to an increased risk of skin cancer.Ultraviolet exposure beyond the minimal dose does not increase your vitamin D production any further. if you avoid getting sunburned, yet have regular sun exposure, you'll have a decreased risk of melanoma. Optimizing your sun exposure in this way also reduces your risk of 16 other common cancers! Remember that humans are designed to have regular sun exposure. As long as you avoid sunburn, sun exposure will help improve your health. For most people living in the United States, two or three hours a week in the summer months, with half your skin area exposed, and noThe ironic thing is that the best time to get the
maximum amount of vitamin D from sun exposure is in the middle of the day, exactly the time that you have been warned to avoid it.you only want to stay out as long it takes for your skin to get slightly pink (if you're light-skinned), no more. amounts to about a half hour on average for light skinned people. The other primary factor to consider when you get your vitamin D from sun exposure is the distance you live from the equator. north of the 30th parallel in the northern hemisphere, or south of the 30th parallel in the southern hemisphere, the sun is not strong enough for about half of the year to produce vitamin D from sun exposure. further you go away from the equator, the shorter the vitamin D seasonTo find the latitude where you live,trythis easy-to-use International Latitude/Longitude Finder Lighter skin allows for greater penetration of UV-B, leading to higher levels of vitamin D. People with pale skin will reach a point after
about 20 minutes of mid-day exposure to UVB light when vitamin D will no This is one of the reasons why breast and prostate cancer rates are so much higher in dark-complected races who live in temperate climates. person with very deep skin pigmentation has natural sun protection that’s similar to a sunscreen with a SPF of 15-30. It can take 3-6 times longer (up to an hour or two) for darker-pigmented skin to reach the maximum production of vitamin D. However, skin pigmentation does not affect the amount of vitamin D that can be obtained through sun Unless you practice regular sunbathing (2 or 3 hours a week), you're not getting enough vitamin D from sun exposure. You really can't get enough from your diet. If you don't compensate by using vitamin D supplements, you almost certainly have low vitamin D levels in yourThe only way to determine this for sure is to get your vitamin D levels tested. You can arrange a vitamin D blood test
with your health care provider, or order an inexpensive home blood test.See Vitamin D Blood Test for more details. — in the summer months, just spend a few hours each week sunbathing. Your skin produces more than 10,000 IU vitamin D with just 30 minutes summer sun exposure in a bathing suit (without sunscreen). months, take natural vitamin D supplements in the form of D-3If your blood levels of vitamin D are low to begin with, it can take a few months to raise your vitamin D levelsand then you'd have to guess whether you're takingMost people tend to use too little, due to unwarranted fears ofA safe way to do it is to take 5,000IU per day for three months, then get a 25-hydroxyvitamin D test to measure your bloodAdjust your dosage from there, so that your blood levels are If you're not getting decent sun exposure, you should be taking at least 1,000-2,000IU as a minimum daily dose. 5,000IU per day is not unreasonable, especially if you live in northern latitudes, are
See Vitamin D Dosage for more details.Vitamin D may be the most important supplement you can take, according to Dr. Joseph Mercola, the internet's most popular alternative-healthFortunately, vitamin D supplements are very inexpensive. I recommend getting vitamin D drops because they're so easy to keep on hand. Carlson Super Daily D3 offers an entire years' supply of vitamin D in just one small bottle that costs about $1 a month. Having an ample supply of vitamin D on hand will also allow you to have extra vitamin D available for treating colds and flu. It's a great place to find all your supplements and natural health products. Just take a look at all they have to offer: • Great Prices — 30% to 40% off most name-brand products• New Customer Discount — save up to $10 off your first order• Loyalty Rewards – Earn 10% credit off your next purchase• Selection — over 35,000 products and 1,200 brand-names• FREE Same-Day Shipping — no charge for orders over $20• International Shipping — discounted rates on orders over $40• Live Customer-Care Service — on the phone or live chat• Bonus Discounts — extra 6%-8% OFF for orders over $40• Rewards Program — earn store credit for referring your