Vitamin D ELISA Kit distributor in your region for pricing Measure vitamin D in plasma or serum Detect the metabolically stable forms 25(OH)D3 and 25(OH)D2 Assay 24 samples in triplicate or 36 samples in duplicate Measure vitamin D levels down to 0.5 ng/ml Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm Vitamin D EIA Kit The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D3 (25(OH)D3). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)2D3), the biologically active form. Active 1,25(OH)2D3 is very short-lived and is rapidly metabolized to its deactivated forms. Vitamin D Immunoassay: Accurate Vitamin D measurement in plasma or serum Cayman’s Vitamin D EIA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum.
Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D3 and 25(OH)D2. Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose. Needed but not supplied: Please download the kit booklet (Milli-Q or equivalent) or any other components are needed for this assay. 80% B/B0: 0.5 ng/ml 50% B/B0: 1-4 ng/ml (25-OH Vitamin D3) 100% (1,25-(OH)2 Vitamin D2) 265% (25-OH Vitamin D2) 200% (1,25-(OH)2 Vitamin D3) 100% (24,25-(OH)2 Vitamin D2) 14% (24,25-(OH)2 Vitamin D3) 4.2% Warning - this product is not for human or veterinary use. As supplied, 1 year from the QC date provided on the Certificate of Analysis, when stored properly A. Krejner, M. Litwiniuk and T. Grzela.
LL-37 but not 25-hydroxy-vitamin D serum level correlates with healing of venous leg ulcers. Epub ahead of print, (2016). L. Zhang, X. Chen, J. Qu, et al. The effects of different intensities of exercise and active vitamin D on mouse bone mass and bone strength. All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution. Toll Free Phone (USA and Canada Only): (888) 526-5351 Direct Phone: (734) 975-3888 We offer you the deep and diverse knowledge of more than 100 in-house scientists and the expertise that comes from more than three decades of designing and developing the tools to advance biomedical research. Cayman is know as a partner to scientists worldwide, responding to your needs with services in: Epigenetic Screening and Profiling Protein Production & Purification You may be eligible to receive a free sample of Vitamin D ELISA Kit under the
VIDAS® 25 OH Vitamin D Total is an automated quantitative test for the determination of 25-hydroxyvitamin D Total in human serum or plasma using the ELFA (Enzyme Linked Fluorescent Assay) technique. It reflects vitamin D produced cutaneously and that obtained from food and supplements for a reliable indication of vitamin D status. An assay of general health status Vitamin D helps the body absorb and use calcium and phosphorus. As a result, vitamin D deficiency causes bone-weakening diseases such as rickets in children and osteoporosis in adults. Recent studies have also established a link between low circulating vitamin D levels and an increased risk of cancer, diabetes and several cardiovascular or autoimmune diseases. Beyond bone health, sufficient exposure to vitamin D - through sun exposure, food and dietary supplements - is essential to strengthen the immune system and maintain general health for optimum wellness(2). The VIDAS® 25 OH Vitamin D Total reference range is compliant with international clinical recommendations(3).
It enables clinicians to confidently establish a patient’s vitamin D status and determine the appropriate supplementation when needed. Following the patient’s status will allow to check the dosage and adjust it. VIDAS® 25 OH Vitamin D Total is very well correlated to the Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) reference method.* It offers the same high degree of precision and is easy to perform in any lab for rapid results. LC-MS/MS 25-OH Vitamin D (ng/mL) - Click to enlarge For the clinical study 343 samples were tested and the correlation between VIDAS 25 OH Vitamin D Total and LC-MS/MS is Y=1.00x+ 041; * See package insert for performance details. Calibration and control only once a month Kit size adapted to your needs Everything needed to perform the test provided in the kit Performed on the instruments of the VIDAS family, well known for their proven reliability Ready-to-use calibrators and controls
1. HOLICK, M.F. (2007). N Engl J Med,357:266-281. 2. GRANT WB, HOLICK MF. Benefits and requirements of vitamin D for optimal health: a review. Altern Med Rev. 2005 Jun;10(2):94-111. 3. INSTITUTE OF MEDICINE. Dietary Reference Intakes for Calcium and Vitamin D. Washington, D.C.: National Academies Press, 2010. Technical specifications for VIDAS® 25 OH Vitamin D Total 25 OH Vitamin D3 100% 25 OH Vitamin D2 91% Find more technical details on . Consult your local bioMérieux representative for product availability in your country. Institute of Medicine Dietary Reference Intakes for Calcium and Vitamin D Moreau E, Dupret-Carruel J, Hausmann M. Measurement of Total 25(OH) Vitamin D using bioMérieux VIDAS®: development of a new assay. Poster presented at AACC Houston, July 2013. Moreau E, Durand C, Villard C, et al. Development of the VIDAS® 25 OH Vitamin D Total assay. Poster presented at Euromedlab Milano, May 2013. Find more scientific and educational resources on .