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2. Briefly describe two differences between generalized and specialized transduction. Generalized transduction -- all regions of chromosome transduced, only chromosomal DNA (i.e., no phage DNA) in transducing particles [2 points]. Specialized transduction -- only specific regions of chromosome located near attachment During generalized transduction, all markers in the genome are transferred at an equal rate, because the phage can package any piece of DNA. Later, a strain was discovered which transduced the lac genes from one strain to another at a much higher frequency. What might this be? SPECIALIZED TRANSDUCTION There are two types of transduction: – generalized transduction: A DNA fragment is transferred from one bacterium to another by a lytic bacteriophage that is now carrying donor bacterial DNA due to an error in maturation during the lytic life cycle. Transmission of genetic variation: transduction. – specialized transduction: A 18 Apr 2011 Bacteriophage P1 and Generalized Transduction in E. coli. HISTORY. Bacteriophage P1 was isolated in 1951 by Luigi Bertani 1. Transduction was discovered by Zinder and Lederberg2, 3 during a search for genetic recombination in Salmonella. Expecting to find conjugative transfer similar that that they Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. An example is the viral transfer of DNA from one bacterium to another. Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation), and it is Genetic transduction is the virus-mediated transfer of nonviral genetic information to a recipient cell. The foreign genes are packaged within a shell of virus proteins and introduced into the recipient by infection. Transduction is traditionally subdivided into two subclasses, specialized and generalized, and the editors of this. Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis. Stoyan Bardarov,1 Svetoslav Bardarov, Jr,1 Martin S. Pavelka, Jr,4. Vasan Sambandamurthy,1,2 Michelle Larsen,1,2 JoAnn Tufariello,1,3. transduction. Transducing bacteriophage particles are formed in donor bacterial cells during phage development. They are of one of two types, distinguished by the nature of the DNA molecule which they carry. Generalized transducing particles, competent to mediate generalized transduction, carry a fragment of host DNA;. Specialized. Transduction of Tetracycline. Resistance by Phage P22 in Salmonella typhimurium. II. Properties of a High-Frequency-Transducing Lysate. RUSSELL I<. CHATC, DAVID BOTSTEIN, TSUTOMU WATANABE,. AND. YASUKO OGATA. Depurtment of Biology, Mussachusetts Institute of Technology, Cambridge, 18 Nov 1985 Specialized Transduction of Pseudomonas aeruginosa PAO by. Bacteriophage D3. MARGARET M. CAVENAGH AND ROBERT V. MILLER*. Department ofBiochemistry and Biophysics and The Program in Molecular Biology, Stritch School ofMedicine, Loyola. University of Chicago,Maywood, Illinois 60153.