Ratio flow jo manual

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FlowJo. Basic Tutorial. FlowJo is software for the analysis of flow cytometry data. This tutorial will quickly introduce you to FlowJo using an example Instructions to guide you through the analysis of a basic immunophenotyping experi- .. Kinetic Analysis - measure the change in signal of dyes, or ratios of dyes, over time. Select your platform (Mac or PC) at the top of the page. For a PC, download the installer.exe file and open it to install the program. Choose to place a shortcut on the desktop and when the installer finishes, double-click on the FlowJo shortcut icon to launch the program. For a Mac, download the installer.zip file and double-. 18 Jan 2013 A: The ratio of Fluo-3 and Fura Red dyes after gating of the individual lymphocyte subsets plotted against time. B: Double-logistic function representing Ca2+ influx kinetics calculated by FacsKin. C: Ca2+ influx kinetics calculated by FlowJo based on median fluorescence data and smoothing. [Color figure FlowJo (Macintosh). Manual for Macintosh. FlowJo (Macintosh). 1 Let FlowJo calculate a ratio of two parameters, add a time parameter (in case you want to do Kinetics analyses), or convert between log and linear FlowJo computes the compensation matrix on control samples much the way you would manually set. 17 May 2006 q Manual Gates. Graph Window. The Graph window is the window in which FlowJo displays graphical data: contour plots, histograms, dot plots, etc. From this window . Derived Parameters. Let FlowJo calculate a ratio of two parameters, add a time parameter (in case you want to do Kinetics analyses),. Ratio: Division can be used to calculate a ratio of any two collected parameters. This is commonly necessary for calcium flux experiments, in which the ratio of Indo-1 fluorescence in two different channels is related to the calcium concentration. You can choose to create a ratio parameter with either linear or logarithmic If this is your first time learning about FlowJo, you should read the pages describing the general concepts behind FlowJo. To see how the current version is different from older versions of the program, click here. As you use the application, you will see that FlowJo is littered with Help buttons. Almost every FlowJo window has FlowJo also produces tables of statistics and graphical reports that summarize multiple data samples with a click of a button. Instructions to guide you through the analysis of a basic immunophenotyping experi- ment are contained in this tutorial. Follow along with the provided data files to get a feel of how FlowJo works! Overview. This page describes some of the basic concepts underlying FlowJo; it is meant to help you familiarize yourself with some of the terminology and the features of FlowJo. As you navigate through the main documentation, you will get more details about all of these topics. In addition, you can follow links to some 9 Aug 2011 This guide is intended for Flow Cytometer users with different degrees of knowledge and experience with Becton Dickinson FACScan™, FACSort™, and FACSCalibur™ flow cytometers. This guide assumes that you have some knowledge of basic flow cytometer theory, how to use the aforementioned flow